[3dem] plunge with vitrobot

[De Carlo, Sacha]

Did you glow discharge using different polarity to test ? Did you clean your carbon foil before using the grid (e.g. with chloroform, ...) ?
This is most likely a sample/support issue and not a vitrobot problem. 

Cheers
Sacha

Sent from my iPhone

> On 26 May 2014, at 00:45, "Xiaofeng Fu" <xiaofeng at brandeis.edu> wrote:
> 
> 
> Dear all,
> 
> Recently we had plunge problem by using vitrobot. Two images are attached: there is always a big droplet in the middle of square, and the ice is very thick at its neighboring area. We varied the GD from 10s to 40s at 24mA, but the grids look similar. The buffer doesn't contain any salt or sugar.
> 
> Look forward to hearing any suggestions or experiences.
> 
> Thanks,
> Xiaofeng
> 
> <1.jpg>
> <2.jpg>
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