[3dem] etomo question dual axis tomogram generation
cristina.suarez at bioquant.uni-heidelberg.de
cristina.suarez at bioquant.uni-heidelberg.de
Thu Mar 13 08:42:36 PDT 2014
Dear colleagues,
I am trying to get a tomogram combination from two single tomograms
using etomo, When I transfer the fiducials from other axis (tomogram
"a" to "b") if I check the seed model the fiducials are wrong and
etomo did not allow me to remove the wrong fiducials in order to put
them in the righ position in the tomogram.
Any of us have an idea of the solution for this problem?
Aditionaly information, when I took the image stacks they were
properly aligned after grid turning 90° and the single axis tomogram
were generated without any problem.
Best regards
Cristina
Cristina Suárez García, PhD.
Electron Microscopy core facility & Dept. of Infectious diseases,
Heidelberg University,
Im Neuenheimer Feld 267, 69120
Heidelberg, Germany
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