[3dem] Help on particles automatic boxing
Brian Gibbons
brian.j.gibbons at gmail.com
Wed Oct 6 12:13:55 PDT 2010
Dogpicker is another optiona, and Tiltpicker works well for tilt pairs.
http://code.google.com/p/appion/downloads/list
For cryo-EM, picking is easier if you take exposure pairs. Take first
exposure close to focus with low dose (for processing) and second exposure
far from focus with higher dose to provide high contrast for picking. Pick
particles from the second, high contrast images and then apply the
coordinates (with adjustment for drift) to window your particles from the
first, low contrast images. I use Spider to do this:
http://www.wadsworth.org/spider_doc/spider/docs/spider.html
Cheers!
Brian
On Sun, Oct 3, 2010 at 2:50 AM, jh <biocjh at gmail.com> wrote:
> Dear all,
>
> Anyone could introduce me with a free software, which can
> automatically box particles from cryo-EM images.
> Thanks.
>
> C.J.
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