[3dem] jobs in electron crystallography
David Stokes
stokes at nyu.edu
Mon Oct 4 06:04:43 PDT 2010
Jobs Available in the Transcontinental EM Initiative for Membrane Protein Structure
A number of jobs are available in our new NIH-funded PSI center for Membrane Protein Structure
Determination (http://cryoem.nysbc.org/temimps). Our's is the only PSI center exclusively committed
to determination of membrane protein structures using electron microscopy. This will involve
development of a number of associated technologies, from high-throughput crystallization screening,
automated imaging in the electron microscope, novel approaches for image analysis. Our overall goal
is to solve structures over the 5-year period of the grant. The target list includes a wide variety
of biologically important proteins, including transporters, pumps, signaling molecules, proteases
and adhesion proteins.
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Electron crystallographer at Mount Sinai School of Medicine - New York, NY
This postdoctoral position will focus on membrane protein structure determination using electron
crystallography. This work will involve de novo crystallization and optimization of 2D crystals,
followed by cryo-electron microscopy and 3D reconstruction using crystallogrpahic software tools.
The department of Structural and Chemical Biology at Mount Sinai School of Medicine
(http://www.mssm.edu/departments-and-institutes/structural-and-chemical-biology/) is well equipped
for all aspects of molecular biology and biochemistry of membrane proteins and has state-of-the-art
equipment for Biophysics and Structural Biology. In addition, we have access to superlative electron
cryo-microscopy facilities at of the New York Structural Biology Center (http://cryoem.nysbc.org).
Candidates must hold a Ph.D. in Molecular Biology, Biochemistry, Biophysics, Structural Biology or
an appropriately related field. A strong research experience in membrane protein structure
determination by electron cryo-microscopy is a requisite. In addition, the candidate must have
strong interpersonal, organizational and communication skills as the work will involve intimate
collaboration with research groups at the New York Structural Biology Center and New York
University. Interested candidates should e-mail a cover letter, curriculum vitae and contact
information for three professional referees to temimps at nysbc.org or to Dr. Iban Ubarretxena
(iban.ubarretxena at mssm.edu).
---------------------------------------------------------
Electron microscopist at New York University, New York, NY
Postdoctoral position is available immediately for an an electron crystallographer in the laboratory
of David Stokes at the Skirball Institute of New York University School of Medicine
(http://skirball.med.nyu.edu/~stokes). This position will focus on structure determination of
tubular crystals formed by membrane proteins. This work will involve optimization of crystals
discovered in the TEMIMPS high-throughput crystallization pipeline, which includes a broad array of
novel membrane protein targets (http://temimps.nysbc.org). Structure determination will then involve
imaging of frozen-hydrated samples and helical reconstruction using either Fourier Bessel methods or
Interative Real Space methods. State-of-the-art cryo-electron microscopes are available both at NYU
and at the New York Structural Biology Center for this work. Software for both approaches to helical
reconstruction are a development focus for members of the TEMIMPS consortium. Candidate should have
a Ph.D. with experience either in electron microscopy or image analysis and an ability to work
effectively within the highly collaborative environment of our multi-institutional consortium. To
apply, email curriculum vitae and contact information for three professional referees to
temimps at nysbc.org or to Dr. David Stokes (stokes at nyu.edu).
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Postdoctoral position at University of Washington School of Medicine, Seattle, WA
Postdoctoral fellow position is available in the laboratory of Tamir Gonen to study the structure
and function of membrane transporters. This position is a mix of membrane biochemistry and electron
crystallography/structure determination as described above. More information can be found at
http://cryoem.washington.edu. Candidates should email curriculum vitae and contact information for
three professional referees to temimps at nysbc.org or to Dr. Tamir Gonen (tgonen at u.washington.edu).
-----------------------------------------------------------
Postdoctoral position at University of Washington School of Medicine, Seattle, WA
Postdoctoral fellow position is available in the laboratory of Tamir Gonen to establish new
protocols for structure determination in electron crystallography. This is a collaborative effort
with Professor David Baker and is computationally intensive. More information can be found at
http://cryoem.washington.edu. Candidates should hold a Ph.D. degree and have relevant experience. To
apply, email curriculum vitae and contact information for three professional referees to
temimps at nysbc.org or to Dr. Tamir Gonen (tgonen at u.washington.edu).
--------------------------------------------------------------
Software Engineer at Case Western Reserve University - Cleveland, Ohio
The EM Facility at the Cleveland Center for Membrane and Structural Biology (CCMSB) and the
Department of Pharmacology at Case Western Reserve University (CWRU) is seeking a Software Engineer.
This individual will be the key researcher for building up the computing infrastructure for the EM
facility of the Cleveland Center for Membrane and Structural Biology, CCMSB. The task includes
building up and maintaining the computational environment for high-speed digital image processing of
electron micrographs using software packages developed by the EM community such as 2DX, EMAN, IPLT,
SPIDER. Further, new software will be developed within the TEMIMPS consortium, tested, and
introduced. Projects are focused on the elucidation of the structure of membrane proteins. Expertise
in Unix, C++ and background in math are required. We provide a great scientific environment within
which the engineer can pick collaborative projects of interest. If you are interested, or for
further information, please contact: Andreas Engel <ahe13 at case.edu> or Vera Moiseenkova-Bell
<vxm102 at case.edu>
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Membrane protein biochemist at Mount Sinai School of Medicine - New York, NY
A position for a highly motivated membrane protein biochemist is available immediately in the
department of Structural and Chemical Biology at Mount Sinai School of Medicine in New York
(http://www.mssm.edu/departments-and-institutes/structural-and-chemical-biology/). This position
will focus on the expression and purification of eukaryotic membrane protein targets for structure
determination by electron cryo-microscopy of 2D crystals. The TEMIMPS consortium (see
http://cryoem.nysbc.org/temimps) is developing automated, high-throughput methods for 2D
crystallization and structure determination of eukaryotic membrane protein targets of biological and
medical relevance. This work will involve expression of eukaryotic membrane proteins in bacteria,
yeast or insect cells. This work will also involve developing biochemical assays to characterize
membrane protein stability and predisposition for 2D crystallization. The department of Structural
and Chemical Biology at Mount Sinai School of Medicine is well equipped for all aspects of molecular
biology and biochemistry of membrane proteins and has state-of-the-art equipment for Biophysics and
Structural Biology. Candidates must hold a Ph.D. in Molecular Biology, Biochemistry, Biophysics,
Structural Biology or an appropriately related field. A strong research experience in expression and
purification of membrane proteins is a requisite. In addition, the candidate must have strong
interpersonal, organizational and communication skills as the work will involve intimate
collaboration with research groups at the New York Structural Biology Center and New York
University. The salary will be commensurate with experience. Interested candidates should e-mail a
cover letter, curriculum vitae and contact information for three professional referees to
temimps at nysbc.org or to Dr. Iban Ubarretxena (iban.ubarretxena at mssm.edu).
--
David L. Stokes
Skirball Institute, NYU Medical Center
tel: 212-263-1580
http://saturn.med.nyu.edu/~stokes
----
New York Structural Biology Center
tel: 212-939-0660 x9116
http://cryoem.nysbc.org/
fax: 646-219-0300
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