[3dem] EM sample in high salt
Florian Hauer
fhauer at gwdg.de
Tue Jul 8 02:31:34 PDT 2008
Nat Methods. 2008 Jan;5(1):53-5. Epub 2007 Dec 23.
On Tue, 08 Jul 2008 00:15:44 +0200, Peijun Zhang <pez7+ at pitt.edu> wrote:
> Hello all,
>
>
> I have an EM specimen which only stabilizes in high salt buffer (1M
> NaCl).
> I would like to carry out cryoEM imaging at lower salt conditions. Does
> anyone have a good protocol to reduce salt to 100mM or less without
> compromising the specimen?
>
>
> Thanks so much,
>
>
> Best,
>
>
> Peijun
>
>
--
Florian Hauer
3D Cryo Electron Microscopy Group
Abt. 10710
Max-Planck Institute for biophysical Chemistry
Am Faßberg 11
37077 Göttingen
Phone: +49 551 201 1302
Fax: +49 551 201 1197
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