[3DEM] Gold Labeling Workshop Announcement
hainfeld at bnl.gov
hainfeld at bnl.gov
Wed Feb 28 17:10:31 PST 2007
Gold Labeling Workshop
Sept. 11-13, 2007
Sponsored by the Brookhaven National Lab STEM (scanning transmission EM)
Facility
A Hands-on Tutorial that will cover both theory and practice. Topics will
include:
Gold nanoparticles: structure, synthesis, and properties
Molecular labeling strategies (to amines, thiols, genetic tags, active
sites, complexes, nucleic acids, peptides)
Ni-NTA-gold
Sizes of gold from 0.8 nm to 30 nm
Isolation of labeled products (various chromatographies and other methods)
Unstained, negatively stained and Cryo samples
Comparison of STEM and TEM
Silver and gold enhancement
Image processing using gold labels
Problems and how to overcome them (e.g., labeling yield/occupancy,
non-specific binding)
Other uses of gold nanoparticles (Western blots, light microscopy, medicine)
Laboratory participation to gold label a protein for EM
What's new
Limited Enrollment (12) due to lab space: A course fee of $650 covers
housing, meals (dorm, cafeteria), and materials.
Proposals to attend will be refereed: submit CV and letter describing you
and your lab's labeling interest.
Optionally describe a sample of yours that, if selected, would be labeled
during the course.
Submit registration request by July 1 to: Dr. James Hainfeld by email:
<mailto:hainfeld at bnl.gov>hainfeld at bnl.gov
Brookhaven National Laboratory, Biology Dept., Bldg. 463, Upton, NY 11973, USA
Tel. 631-344-3372 * Fax. 631-344-3407
Dr. James F. Hainfeld * hainfeld at bnl.gov
Brookhaven National Laboratory * Biology Dept. * Bldg. 463 * Upton,
NY 11973 USA
Tel. 631-344-3372 * Fax. 631-344-3407
www.biology.bnl.gov/stem/stem.html
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