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Gold Labeling Workshop<br><br>
Sept. 11-13, 2007<br>
Sponsored by the Brookhaven National Lab STEM (scanning transmission EM)
Facility<br>
A Hands-on Tutorial that will cover both theory and practice.
Topics will include:<br>
Gold nanoparticles: structure, synthesis, and properties<br>
Molecular labeling strategies (to amines, thiols, genetic tags, active
sites, complexes, nucleic acids, peptides)<br>
Ni-NTA-gold<br>
Sizes of gold from 0.8 nm to 30 nm<br>
Isolation of labeled products (various chromatographies and other
methods)<br>
Unstained, negatively stained and Cryo samples<br>
Comparison of STEM and TEM<br>
Silver and gold enhancement<br>
Image processing using gold labels<br>
Problems and how to overcome them (e.g., labeling yield/occupancy,
non-specific binding)<br>
Other uses of gold nanoparticles (Western blots, light microscopy,
medicine)<br>
Laboratory participation to gold label a protein for EM<br>
What’s new<br>
<br>
Limited Enrollment (12) due to lab space: A course fee of $650
covers housing, meals (dorm, cafeteria), and materials.<br>
Proposals to attend will be refereed: submit CV and letter describing you
and your lab’s labeling interest.<br>
Optionally describe a sample of yours that, if selected, would be labeled
during the course.<br>
Submit registration request by July 1 to: Dr. James Hainfeld by email:
<a href="mailto:hainfeld@bnl.gov">hainfeld@bnl.gov</a><br>
Brookhaven National Laboratory, Biology Dept., Bldg. 463, Upton, NY
11973, USA<br>
Tel. 631-344-3372 * Fax. 631-344-3407<br><br>
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Dr. James F. Hainfeld * hainfeld@bnl.gov<br>
Brookhaven National Laboratory * Biology Dept. *
Bldg. 463 * Upton, NY 11973 USA<br>
Tel. 631-344-3372 * Fax. 631-344-3407<br>
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