[3DEM] number of required images
Marin van Heel
m.vanheel at imperial.ac.uk
Thu Mar 2 13:22:57 PST 2006
Dear Leandro,
For the derivation of the formula you may want to have a look at:
Marin van Heel and George Harauz, *Resolution criteria for three
dimensional reconstructions*, /Optik/ *73* (1986) 119-122.
In that paper we also derived a formula for truly random orientations of
projections which is more favourable than the Crowther's formula.
d >= 2 * D / N due to the more favourable distribution of projection
directions.
Hope this helps,
Marin van Heel
======================================
Leandro ESTROZI wrote:
> **** Messages to this list are automatically archived ***
> **** Please limit quoting of previous postings to the bare minimum ****
>
> Hi,
>
> Does anybody know from where the formula below is derived?
>
> N = pi * D / d
>
> where N is the number of required images in order to make a
> reconstruction with diameter D at resolution d.
>
> Many people cite Crowther's Nature paper (1970) but that formula does
> not appear therein.
>
> Thanks,
>
> Leandro.
>
--
================================================================
Marin van Heel
Professor of Structural Biology
Imperial College London
Department of Biological Sciences
Biochemistry Building
South Kensington Campus
London SW7 2AY
UK
Tel: + 44 (0)20 7594 5316
Fax: + 44 (0)20 7594 5317
e_mail: m.vanheel at ic.ac.uk
and: Osnat Lipson <vanheel.office at ic.ac.uk>
web: www.cbem.ic.ac.uk
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