TEM: sucrose in cryo-specimens
Sacha De Carlo
sacha.decarlo at lau.unil.ch
Wed Jan 29 23:13:37 PST 2003
Dear people,
there is nice data in the literature about sucrose/trehalose comparison
for thin film preparations for cryo-em, see the following :
De Carlo S., Adrian M., Kälin P., Mayer J. M. and Dubochet J. (1999)
Unexpected property of trehalose as observed by cryo-electron
microscopy. Journal of Microscopy, 196, 40-45.
Yours sincerely,
Sacha
Le Mercredi, 29 jan 2003, à 18:08 Europe/Zurich, D. Prabha Dias, PhD a
écrit :
> I've been able to freeze samples with >5% sucrose by washing the
> sample once on the grid. I would think 1% sucrose would wash out very
> easily, if you need to do it at all. As for image quality, I can only
> tell you that they are qualitatively comparable to samples without
> sucrose. Does anyone have any data on this?
>
> -Prabha
>
> At 15:49 +0100 1/29/03, Philip Koeck wrote:
>> Me again,
>>
>> would 1% sucrose be a problem in frozen hydrated specimens (single
>> particles)?
>> If so is it necessary to dyalize it out or is it sufficient to wash
>> it of
>> the grid before
>> plunge-freezing?
>>
>> Thanks in advance,
>>
>> Philip
>>
>> Philip Koeck
>> Svdertvrns Hvgskola and
>> Karolinska Institutet
>> Dept. of Bioscience at Novum
>> S-14157 Huddinge
>> Sweden
>> phone: +46-8-6089186
>> fax: +46-8-6089290
>
> --
> D. Prabha Dias, Ph.D.
> Division of Biology, California Institute of Technology
> M/C 114-96, BIO19
> 1200 East California Blvd.
> Pasadena, CA 91125
>
> phone: (626)395-8829
> fax: (626)395-5730
> email: prabha at caltech.edu
>
> ----------------------------------------------------
> To unsubscribe from this e-mail list, send mail to
> majordomo at sdsc.edu
> with the words
> unsubscribe 3dem
> in the body of the message.
> Do not send unsubscribe messages to the entire list
> -----------------------------------------------------
>
>
==================================================
Sacha De Carlo, Ph.D
Département de Génomique et Biologie Structurales
Institut de Génétique et Biologie Moléculaire et Cellulaire
UMR 7104 - ULP Strasbourg
1, rue Laurent Fries
F--67404 ILLKIRCH
==================================================
-------------- next part --------------
A non-text attachment was scrubbed...
Name: not available
Type: text/enriched
Size: 2122 bytes
Desc: not available
Url : http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20030130/86a971fc/attachment.bin
More information about the 3dem
mailing list