TEM: sucrose in cryo-specimens
D. Prabha Dias, PhD
prabha at caltech.edu
Wed Jan 29 09:08:57 PST 2003
I've been able to freeze samples with >5% sucrose by washing the
sample once on the grid. I would think 1% sucrose would wash out
very easily, if you need to do it at all. As for image quality, I can
only tell you that they are qualitatively comparable to samples
without sucrose. Does anyone have any data on this?
-Prabha
At 15:49 +0100 1/29/03, Philip Koeck wrote:
>Me again,
>
>would 1% sucrose be a problem in frozen hydrated specimens (single
>particles)?
>If so is it necessary to dyalize it out or is it sufficient to wash it of
>the grid before
>plunge-freezing?
>
>Thanks in advance,
>
>Philip
>
>Philip Koeck
>Svdertvrns Hvgskola and
>Karolinska Institutet
>Dept. of Bioscience at Novum
>S-14157 Huddinge
>Sweden
>phone: +46-8-6089186
>fax: +46-8-6089290
--
D. Prabha Dias, Ph.D.
Division of Biology, California Institute of Technology
M/C 114-96, BIO19
1200 East California Blvd.
Pasadena, CA 91125
phone: (626)395-8829
fax: (626)395-5730
email: prabha at caltech.edu
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