[3dem] Issue with Superdex 200 Increase Columns - Seeking

Mon Jan 13 06:17:55 PST 2025

Dear Benjamin, Carlos, Nick, Sunny, Jason, and Community,

Thank you all for your valuable feedback. I have forwarded the information
you provided to the person addressing the issue in my lab.

It's reassuring to know that this issue is not unique to our lab and is
being encountered elsewhere as well. Your insights have been very helpful.

Thank you again for taking the time to help me. 

Wishing you all a wonderful evening!

Kind regards,
Juan

-----Original Message-----
From: 3dem <3dem-bounces at ncmir.ucsd.edu> On Behalf Of Vollmer, Benjamin
Sent: Monday, 13 January 2025 00:27
To: 3dem at ncmir.ucsd.edu
Subject: Re: [3dem] Issue with Superdex 200 Increase Columns - Seeking

Dear Juan,

About a year ago we had exactly the same issue. Our superose 6 3.2 increase
was fairly new and just like in your images the lower part split while
hanging in the cold room over the weekend (not in use). Contacting Sigma did
not help much as they claimed user error. I also tried to get a replacement
for the plastic casing, as the glass column inside seemed fine, but these
are not available (they used to make/sell metal ones). Finally, the Cytiva
rep took care of it. Cytiva then contacted Sigma and arranged a replacement
(apparently other people had the same issue). So I would try to talk to your
cytiva rep directly, as writing emails and phone calls with the service
centre were not very successful.

Good luck, I hope you get them replaced! 

All the best,
Ben

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Sent: Friday, 10 January, 2025 21:00:02
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Today's Topics:

   1. Re: Issue with Superdex 200 Increase Columns - Seeking
      Community Advice (Juan Tasis Galarza) (Kikuti Carlos)

----------------------------------------------------------------------

Message: 1
Date: Fri, 10 Jan 2025 17:15:02 +0000
From: Kikuti Carlos <Carlos.Kikuti at curie.fr>
To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
Subject: Re: [3dem] Issue with Superdex 200 Increase Columns - Seeking
	Community Advice (Juan Tasis Galarza)
Message-ID:

<PAYP264MB3486E78CEB1F458A0506DB04871C2 at PAYP264MB3486.FRAP264.PROD.OUTLOOK.C
OM>

Content-Type: text/plain; charset="windows-1252"

What ???? Qu? es eso ???

I?m a Cytiva (almost) unconditional fan. I?ve never seen this. You have the
coiled plug which is supposed to equilibrate any pressure variation, so this
does not make much sense. Maybe the transportation company is going too
harsh on them? Maybe they are coming already with partial fractures, and the
HPLC or the temperature variation finishes the job?? That glass is quite
strong, hombre ? but it is still glass, so it breaks if you hit sideways.

We have small and large Superdex columns (up to 26/60, ~300 ml of resin),
you imagine how fragile they are to temperature variations. Even to move in
and out from the cold room we attach the coil plug. The only times I?ve seen
them breaking were after mishandling (user?s fault, pressure alarm higher
than what the manual says, no pressure alarm, or clumsy hands). Never had a
single one already broken on delivery.

I?d immediately call the commercial representative ? if you are using an
intermediate (Sigma, VWR, whatever) call both Cytiva?s and that
representative. They might consider changing the transportation company or
simply the packaging ? if that is really the reason, and I can?t see what
else could be.

Suerte,

---------------------------------------------------
Carlos KIKUTI, PhD
UMR144 - CNRS - Institut Curie
Pavillon Trouillet Rossignol
26 Rue d?Ulm - 75005 Paris, France
carlos.kikuti at curie.fr<mailto:carlos.kikuti at curie.fr>

Message: 3
Date: Fri, 10 Jan 2025 10:51:29 +0100
From: Juan Tasis Galarza <jtasis at ucm.es>
To: CCPEM at jiscmail.ac.uk, 3dem at ncmir.ucsd.edu
Subject: [3dem] Issue with Superdex 200 Increase Columns - Seeking
        Community       Advice
Message-ID: <AA80A835-0CF2-47C1-990B-7104633BD893 at ucm.es>
Content-Type: text/plain; charset="utf-8"

?Happy New Year everyone,

I wanted to reach out with a question regarding Cytiva purification columns
to see if any of you have experienced a similar issue with your equipment.

In our lab, we've encountered a recurring problem where several Superdex 200
Increase 3.2/300 columns have broken spontaneously at the same location
(exit position, look at the attached images). This has occurred under
various circumstances: some while in use, others during storage without use,
and, incredibly, even a few straight out of the box after purchase.

We?ve contacted Cytiva, but they?ve responded that their equipment is in
good condition.
I?m curious to know if anyone else has faced similar problems with these
columns and, if so, what measures you?ve taken to address or prevent this
issue.

I?d appreciate any insights or advice you can offer.

Best regards,
Juan

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