[3dem] "Traditional" Plastic Thin-Sectioning

[David Michael Belnap]

All of us in the community are happy about the great improvements in and expansion of cryo-EM, but “traditional”, plastic-sectioning TEM still has great usefulness.  Plastic sections can help determine feasibility for cryo-studies or may answer what people need to know faster and easier.  I have heard of labs who would like to do sectioning for TEM but are hindered by long queues or lack of expertise or equipment.  Our EM core laboratory has expertise in the following sectioning techniques, and we offer our services to you:

1. Plastic-embedding TEM

  *   processing, cutting, and imaging
  *   aldehyde-fixed or high-pressure frozen, freeze-substituted samples
  *   thin-sectioning (diamond knife)
  *   can also do thick-sectioning (glass knife) for light microscopy

2. Immunogold TEM

  *   Pre-embedding method
  *   Tokuyashu cryo-sectioning method

We pledge a quick turnaround.  We also are open to helping you with only part of the process.  For example, we can just process and cut specimens and send them to you to image.

Please see our website (https://urldefense.com/v3/__https://cores.utah.edu/electron-microscopy/__;!!Mih3wA!HmmyDo_zjweX1DzLh-hLoBeiwjfavgiYi0e_mXy7Sdj4i423jjlmCerS1peV_vf74Hu9EEbqHwRlcHv4mupxT8EWJyQ$ ) or contact me if you are interested.

Best wishes,

David

=======================================
David M. Belnap
Electron Microscopy Core Laboratory
Department of Biochemistry
School of Biological Sciences
2501 SMBB
University of Utah
Salt Lake City, Utah 84112 USA
Phone  801.585.1242
FAX  801.587.3077
David.Belnap at utah.edu<mailto:David.Belnap at utah.edu>
=======================================

-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20250227/8b4c5bd9/attachment.html>