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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Georgia",serif;color:black">All of us in the community are happy about the great improvements in and expansion of cryo-EM, but “traditional”, plastic-sectioning TEM still has great usefulness.
Plastic sections can help determine feasibility for cryo-studies or may answer what people need to know faster and easier. I have heard of labs who would like to do sectioning for TEM but are hindered by long queues or lack of expertise or equipment. Our
EM core laboratory has expertise in the following sectioning techniques, and we offer our services to you:</span><span style="font-size:13.5pt;color:black"><o:p></o:p></span></p>
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<span style="font-size:11.0pt;font-family:"Georgia",serif;color:black"> </span><span style="font-size:13.5pt;color:black"><o:p></o:p></span></p>
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<span style="font-size:11.0pt;font-family:"Georgia",serif;color:black">1. Plastic-embedding TEM</span><span style="font-size:13.5pt;color:black"><o:p></o:p></span></p>
<ul style="margin-top:0in;caret-color: rgb(0, 0, 0);font-variant-caps: normal;orphans: auto;text-align:start;widows: auto;-webkit-text-stroke-width: 0px;word-spacing:0px" type="disc">
<li class="MsoNormal" style="color:black;mso-list:l1 level1 lfo1"><span style="font-size:11.0pt;font-family:"Georgia",serif">processing, cutting, and imaging</span><o:p></o:p></li><li class="MsoNormal" style="color:black;mso-list:l1 level1 lfo1"><span style="font-size:11.0pt;font-family:"Georgia",serif">aldehyde-fixed or high-pressure frozen, freeze-substituted samples</span><o:p></o:p></li><li class="MsoNormal" style="color:black;mso-list:l1 level1 lfo1"><span style="font-size:11.0pt;font-family:"Georgia",serif">thin-sectioning (diamond knife)</span><o:p></o:p></li><li class="MsoNormal" style="color:black;mso-list:l1 level1 lfo1"><span style="font-size:11.0pt;font-family:"Georgia",serif">can also do thick-sectioning (glass knife) for light microscopy</span><o:p></o:p></li></ul>
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<span style="font-size:11.0pt;font-family:"Georgia",serif;color:black"> </span><span style="font-size:13.5pt;color:black"><o:p></o:p></span></p>
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<span style="font-size:11.0pt;font-family:"Georgia",serif;color:black">2. Immunogold TEM</span><span style="font-size:13.5pt;color:black"><o:p></o:p></span></p>
<ul style="margin-top:0in;caret-color: rgb(0, 0, 0);font-variant-caps: normal;orphans: auto;text-align:start;widows: auto;-webkit-text-stroke-width: 0px;word-spacing:0px" type="disc">
<li class="MsoNormal" style="color:black;mso-list:l0 level1 lfo2"><span style="font-size:11.0pt;font-family:"Georgia",serif">Pre-embedding method</span><o:p></o:p></li><li class="MsoNormal" style="color:black;mso-list:l0 level1 lfo2"><span style="font-size:11.0pt;font-family:"Georgia",serif">Tokuyashu cryo-sectioning method</span><o:p></o:p></li></ul>
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<span style="font-size:11.0pt;font-family:"Georgia",serif;color:black"> </span><span style="font-size:13.5pt;color:black"><o:p></o:p></span></p>
<p class="MsoNormal" style="caret-color: rgb(0, 0, 0);font-variant-caps: normal;orphans: auto;text-align:start;widows: auto;-webkit-text-stroke-width: 0px;word-spacing:0px">
<span style="font-size:11.0pt;font-family:"Georgia",serif;color:black">We pledge a quick turnaround. We also are open to helping you with only part of the process. For example, we can just process and cut specimens and send them to you to image.</span><span style="font-size:13.5pt;color:black"><o:p></o:p></span></p>
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<span style="font-size:11.0pt;font-family:"Georgia",serif;color:black"> </span><span style="font-size:13.5pt;color:black"><o:p></o:p></span></p>
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<span style="font-size:11.0pt;font-family:"Georgia",serif;color:black">Please see our website (<a href="https://urldefense.com/v3/__https://cores.utah.edu/electron-microscopy/__;!!Mih3wA!HmmyDo_zjweX1DzLh-hLoBeiwjfavgiYi0e_mXy7Sdj4i423jjlmCerS1peV_vf74Hu9EEbqHwRlcHv4mupxT8EWJyQ$" title="https://cores.utah.edu/electron-microscopy/">https://cores.utah.edu/electron-microscopy/</a>) or contact
me if you are interested.</span><span style="font-size:13.5pt;color:black"><o:p></o:p></span></p>
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<span style="font-size:11.0pt;font-family:"Georgia",serif;color:black"> </span><span style="font-size:13.5pt;color:black"><o:p></o:p></span></p>
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<span style="font-size:11.0pt;font-family:"Georgia",serif;color:black">Best wishes,</span><span style="font-size:13.5pt;color:black"><o:p></o:p></span></p>
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<span style="font-size:11.0pt;font-family:"Georgia",serif;color:black"> </span><span style="font-size:13.5pt;color:black"><o:p></o:p></span></p>
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<span style="font-size:11.0pt;font-family:"Georgia",serif;color:black">David</span><span style="font-size:13.5pt;color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Georgia",serif;mso-ligatures:none">=======================================<br>
David M. Belnap<br>
Electron Microscopy Core Laboratory<br>
Department of Biochemistry<br>
School of Biological Sciences<br>
2501 SMBB<br>
University of Utah<br>
Salt Lake City, Utah 84112 USA<br>
Phone 801.585.1242<br>
FAX 801.587.3077<br>
<a href="mailto:David.Belnap@utah.edu" target="_blank" title="mailto:David.Belnap@utah.edu"><span style="color:#0563C1">David.Belnap@utah.edu</span></a><br>
=======================================</span><span style="font-size:11.0pt;font-family:"Georgia",serif"><o:p></o:p></span></p>
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