[3dem] The 9th Brazil School for Single Particle Cryo-EM

[Marin van Heel]

*A**fter a gap of 5 years due to the Covid-19 pandemic we revived the
Brazil schools!*

16 Oct - 28 Oct 2024, Mairiporã, SP Brazil (https://urldefense.com/v3/__http://www.brazil-school.org__;!!Mih3wA!DqMivg40MqFunXRkUdS9szoSkR04uWoEmHCjGV6pqKWR4PhJPkClUp3NwqGLN-uDJq8Nj8cpFS5YCWuAoZvDUe78RA$ )

https://urldefense.com/v3/__https://www.linkedin.com/feed/update/urn:li:activity:7360051727942791169/__;!!Mih3wA!DqMivg40MqFunXRkUdS9szoSkR04uWoEmHCjGV6pqKWR4PhJPkClUp3NwqGLN-uDJq8Nj8cpFS5YCWuAoZuNtsfWAA$ 

*Registration*: *Until **August 24, 2025*

https://urldefense.com/v3/__https://pages.cnpem.br/singleparticle/registrations/__;!!Mih3wA!DqMivg40MqFunXRkUdS9szoSkR04uWoEmHCjGV6pqKWR4PhJPkClUp3NwqGLN-uDJq8Nj8cpFS5YCWuAoZv62dZL_w$ 


The *international* series of *Brazil Schools for Single Particle Cryo-EM*
started in 2005 and many of the participants and instructors of those
Schools call it the decisive event of their *EM* career. The school is
an *intensive
12-days workshop* aimed at providing a sound theoretical and intuitive
understanding of *Cryo-EM* principles. This school amalgamates theoretical
teaching with practical courses on *your own computer* (to be specified
separately) and maybe even with *your own data set*. The high ratio of 1:2
between instructor/ lecturers and participants, ensures the necessary
attention for the *participants' specific problems*. The processing
methodologies and the software used, are applicable to many sciences
where: *1D;
2D; 3D; *and even* 4D*, data are processed. We are introducing *new
advanced topics* at the cutting edge of *Cryo-EM*. The new *electron-counting
cameras* not only provide excellent results; they also challenge the
very *foundations
of physics*! New insights help resolving the *measurement problem *in
*Classical
Physics* and *Quantum Mechanics*.

*All the best!  Marin van Heel*

*Topics Distributed over various speakers (to be announced) and lectures**:*

*Specimen preparation for single-particle Cryo-EM; Optimizing the EM data
collection; Filtering and data normalization; Best hardware for processing
large Cryo-EM data sets; What is Information in measurements (and not just
in EM); The Fourier transform in 1D – 2D – 3D; **4D Processing of all known
SARS-CoV2 structures; **Properties of the Fourier transform; **Multivariate
Statistical Analysis (used for everything…);* *Unsupervised automatic
classification; **The principles of Eigen-Filtering; **Automatic Particle
selection; **CTF correction using new approaches; **Multi-reference
alignments in 2D/3D;* *Angular reconstitution: a-posteriori Euler angle
determination; **What are Maximum Likelihood approaches; **Exact-Filter
Three-dimensional reconstruction;* *Projection matching;* *Alignment and
refinements 2D/3D/4D;* *1D/2D/3D resolution and Information criteria;* *3D
fitting of atomic coordinates;* *Image processing software (IMAGIC-4D+other
useful software);* *Parallel processing using MPI; **GPUs and Cryo-EM data
processing;* *What are the advantages of Single-Electron counting; **What
are the Quantum limits of Single-Electron counting?*
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20250814/f14f0399/attachment.html>