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<div><p class="MsoNormal" style="margin:0cm 0cm 8pt;line-height:107%;font-size:11pt;font-family:"Calibri",sans-serif;text-align:center"><font size="4"><b><u><span lang="EN-GB">A</span></u></b><b><u><span lang="EN-GB" style="line-height:107%">fter a gap of 5 years due
to the Covid-19 pandemic we revived the Brazil schools!</span></u></b></font><span lang="EN-GB"><span></span></span></p><p class="MsoNormal" style="margin:0cm 0cm 8pt;line-height:107%;font-size:11pt;font-family:"Calibri",sans-serif;text-align:center"><span lang="EN-GB">16 Oct - 28 Oct 2024, Mairiporã, SP Brazil
(<a href="https://urldefense.com/v3/__http://www.brazil-school.org__;!!Mih3wA!DqMivg40MqFunXRkUdS9szoSkR04uWoEmHCjGV6pqKWR4PhJPkClUp3NwqGLN-uDJq8Nj8cpFS5YCWuAoZvDUe78RA$" style="color:rgb(5,99,193);text-decoration:underline" target="_blank">www.brazil-school.org</a>)<span></span></span></p>

<p class="MsoNormal" style="margin:0cm 0cm 8pt;line-height:107%;font-size:11pt;font-family:"Calibri",sans-serif;text-align:center"><span lang="EN-GB"><a href="https://urldefense.com/v3/__https://www.linkedin.com/feed/update/urn:li:activity:7360051727942791169/__;!!Mih3wA!DqMivg40MqFunXRkUdS9szoSkR04uWoEmHCjGV6pqKWR4PhJPkClUp3NwqGLN-uDJq8Nj8cpFS5YCWuAoZuNtsfWAA$" style="color:rgb(5,99,193);text-decoration:underline" target="_blank">https://www.linkedin.com/feed/update/urn:li:activity:7360051727942791169/</a><span></span></span></p>

<p class="MsoNormal" style="margin:0cm 0cm 8pt;line-height:107%;font-size:11pt;font-family:"Calibri",sans-serif;text-align:center"><b><u><span lang="EN-IN" style="font-family:"Times New Roman",serif">Registration</span></u></b><span lang="EN-IN" style="font-family:"Times New Roman",serif">: <b>Until </b></span><b><span lang="EN-GB" style="font-family:"Times New Roman",serif">August 24, 2025</span></b><b><span lang="EN-IN" style="font-family:"Times New Roman",serif"><span></span></span></b></p>

<p class="MsoNormal" style="margin:0cm 0cm 8pt;line-height:107%;font-size:11pt;font-family:"Calibri",sans-serif;text-align:center"><span lang="EN-GB"><a href="https://urldefense.com/v3/__https://pages.cnpem.br/singleparticle/registrations/__;!!Mih3wA!DqMivg40MqFunXRkUdS9szoSkR04uWoEmHCjGV6pqKWR4PhJPkClUp3NwqGLN-uDJq8Nj8cpFS5YCWuAoZv62dZL_w$" style="color:rgb(5,99,193);text-decoration:underline" target="_blank"><span lang="EN-IN" style="font-family:"Times New Roman",serif">https://pages.cnpem.br/singleparticle/registrations/</span></a></span><span lang="EN-IN" style="font-family:"Times New Roman",serif"><span></span></span></p>

<p class="MsoNormal" style="margin:0cm 11.35pt 6pt;text-align:justify;line-height:normal;font-size:11pt;font-family:"Calibri",sans-serif"><span lang="EN-IN" style="font-family:"Times New Roman",serif"><br></span></p><p class="MsoNormal" style="margin:0cm 11.35pt 6pt;text-align:justify;line-height:normal;font-size:11pt;font-family:"Calibri",sans-serif"><span lang="EN-IN" style="font-family:"Times New Roman",serif">The
<b><i>international</i></b> series of <b><i>Brazil Schools for Single Particle Cryo-EM</i></b>
started in 2005 and many of the participants and instructors of those Schools call
it the decisive event of their <b><i>EM</i></b> career. The school is an <b><i>intensive
12-days workshop</i></b> aimed at providing a sound theoretical and intuitive
understanding of <b><i>Cryo-EM</i></b> principles. This school amalgamates
theoretical teaching with practical courses on <b><i>your own computer</i></b> (to
be specified separately) and maybe even with <b><i>your own data set</i></b>. The
high ratio of 1:2 between instructor/ lecturers and participants, ensures the
necessary attention for the <b><i>participants' specific problems</i></b>. The processing
methodologies and the software used, are applicable to many sciences where: <b>1<i>D;
</i>2<i>D; </i>3<i>D; </i></b>and even<b><i> </i>4<i>D</i></b>, data are processed.
We are introducing <b><i>new advanced topics</i></b> at the cutting edge of <b><i>Cryo-EM</i></b>.
The new <b><i>electron-counting cameras</i></b> not only provide excellent results;
they also challenge the very <b><i>foundations of physics</i></b>! New insights
help resolving the <b><i>measurement problem </i></b>in <b><i>Classical Physics</i></b> and <b><i>Quantum Mechanics</i></b>.<span></span></span></p><p class="MsoNormal" align="center" style="margin:0cm 0cm 6pt;text-align:center;line-height:normal;font-size:11pt;font-family:"Calibri",sans-serif"><b><span lang="EN-IN" style="font-size:12pt;font-family:"Times New Roman",serif">All the best!<span>  </span>Marin van Heel<span></span></span></b></p>

<p class="MsoNormal" align="center" style="text-align:center;line-height:107%;font-size:11pt;font-family:"Calibri",sans-serif"><b><u><span style="font-size:10pt;line-height:107%;font-family:"Times New Roman",serif">Topics Distributed over various speakers (to be
announced) and lectures</span></u></b><b><span style="font-size:10pt;line-height:107%;font-family:"Times New Roman",serif">:<span></span></span></b></p>

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<div><p style="margin:0cm 0cm 0cm 31.5pt;line-height:107%;font-size:11pt;font-family:"Calibri",sans-serif;text-align:left"><b><span style="font-size:8pt;line-height:107%;font-family:"Times New Roman",serif">Specimen
preparation for single-particle Cryo-EM; Optimizing
the EM data collection; Filtering and
data normalization; Best hardware
for processing large Cryo-EM data sets; What is <i>Information
in measurements</i> (and not just in EM); The Fourier
transform in 1D – 2D – 3D; </span></b><b><span style="font-size:8pt;line-height:107%;font-family:"Times New Roman",serif">4D Processing
of all known SARS-CoV2 structures; </span></b><b><span style="font-size:8pt;line-height:107%;font-family:"Times New Roman",serif">Properties of
the Fourier transform; </span></b><b><span style="font-size:8pt;line-height:107%;font-family:"Times New Roman",serif">Multivariate
Statistical Analysis (used for everything…);</span></b><span style="font-size:8pt;line-height:107%;font-family:Symbol"><span><span style="font:7pt "Times New Roman""> </span></span></span><b><span style="font-size:8pt;line-height:107%;font-family:"Times New Roman",serif">Unsupervised
automatic classification; </span></b><b><span style="font-size:8pt;line-height:107%;font-family:"Times New Roman",serif">The
principles of Eigen-Filtering; </span></b><b><span style="font-size:8pt;line-height:107%;font-family:"Times New Roman",serif">Automatic
Particle selection; </span></b><b><span style="font-size:8pt;line-height:107%;font-family:"Times New Roman",serif">CTF
correction using new approaches; </span></b><b><span style="font-size:8pt;line-height:107%;font-family:"Times New Roman",serif">Multi-reference
alignments in 2D/3D;</span></b><span style="font-size:8pt;line-height:107%;font-family:Symbol"><span><span style="font:7pt "Times New Roman""> </span></span></span><b><span style="font-size:8pt;line-height:107%;font-family:"Times New Roman",serif">Angular
reconstitution: a-posteriori Euler angle determination; </span></b><b><span style="font-size:8pt;line-height:107%;font-family:"Times New Roman",serif">What are
Maximum Likelihood approaches; </span></b><b><span style="font-size:8pt;line-height:107%;font-family:"Times New Roman",serif">Exact-Filter
Three-dimensional reconstruction;</span></b><span style="font-size:8pt;line-height:107%;font-family:Symbol"><span><span style="font:7pt "Times New Roman""> </span></span></span><b><span style="font-size:8pt;line-height:107%;font-family:"Times New Roman",serif">Projection
matching;</span></b><span style="font-size:8pt;line-height:107%;font-family:Symbol"><span><span style="font:7pt "Times New Roman""> </span></span></span><b><span style="font-size:8pt;line-height:107%;font-family:"Times New Roman",serif">Alignment and
refinements 2D/3D/4D;</span></b><span style="font-size:8pt;line-height:107%;font-family:Symbol"><span><span style="font:7pt "Times New Roman""> </span></span></span><b><span style="font-size:8pt;line-height:107%;font-family:"Times New Roman",serif">1D/2D/3D
resolution and Information criteria;</span></b><span style="font-size:8pt;line-height:107%;font-family:Symbol"><span><span style="font:7pt "Times New Roman""> </span></span></span><b><span style="font-size:8pt;line-height:107%;font-family:"Times New Roman",serif">3D fitting of
atomic coordinates;</span></b><span style="font-size:8pt;line-height:107%;font-family:Symbol"><span><span style="font:7pt "Times New Roman""> </span></span></span><b><span style="font-size:8pt;line-height:107%;font-family:"Times New Roman",serif">Image
processing software (IMAGIC-4D+other useful software);</span></b><span style="font-size:8pt;line-height:107%;font-family:Symbol"><span><span style="font:7pt "Times New Roman""> </span></span></span><b><span style="font-size:8pt;line-height:107%;font-family:"Times New Roman",serif">Parallel
processing using MPI; </span></b><b><span style="font-size:8pt;line-height:107%;font-family:"Times New Roman",serif">GPUs and
Cryo-EM data processing;</span></b><span style="font-size:8pt;line-height:107%;font-family:Symbol"><span><span style="font:7pt "Times New Roman""> </span></span></span><b><span style="font-size:8pt;line-height:107%;font-family:"Times New Roman",serif">What are the
advantages of Single-Electron counting; </span></b><b><span style="font-size:8pt;line-height:107%;font-family:"Times New Roman",serif">What are the Quantum
limits of Single-Electron counting?</span></b></p></div>

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