[3dem] 3dem Digest, Vol 206, Issue 27
Qiang Guo
guoq.tiger at gmail.com
Fri Oct 25 03:47:58 PDT 2024
Dear Liguo,
I believe you're referring to the gas cooling tank. If the microscope is
used frequently, emptying the tank daily is unnecessary and wasteful. We
typically refill it daily instead.
If emptying is essential in your lab, maybe consider a Polara-style setup:
a long tube to the bottom, a lid that could seal the tank, and a heater.
Best,
Qiang
------------------------------------------
Message: 3
Date: Thu, 24 Oct 2024 10:41:26 -0400
From: Liguo Wang <wanguw11 at gmail.com>
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] LN2 Tank Handling for Aquilos 2 cryo-FIB
Message-ID: <045c8097-f9ce-4e42-b4b0-dbbb6160fc2e at gmail.com>
Content-Type: text/plain; charset=UTF-8; format=flowed
Dear 3DEM community,
We?ve been using the Aquilos 2 cryo-FIB and encountered a challenge with
the LN2 tank. After daily use, a significant amount of LN2 remains, and
we need to empty the tank and dry it using N2 gas. However, pouring the
LN2 out of the tank is quite difficult and could potentially damage the
plastic ring/cap.
Does anyone have a better solution for safely and efficiently handling this?
Thank you in advance for your help.
Liguo Wang
LBMS at BNL
<3dem-request at ncmir.ucsd.edu> 于2024年10月24日周四 23:25写道：
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> Today's Topics:
>
> 1. 4 PhD and postdoctoral postiions available in the University
> of Helsinki, Finland, DL 7th November 2024 (Butcher, Sarah)
> 2. cryoEM staff microscopist position available - NYSBC, NYC,
> USA (Ed Eng)
> 3. LN2 Tank Handling for Aquilos 2 cryo-FIB (Liguo Wang)
> 4. Q4 2024 National Network for Cryo-Electron Tomography
> Application Deadline Extension (Alex Cotten)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Thu, 24 Oct 2024 02:06:17 +0000
> From: "Butcher, Sarah" <sarah.butcher at helsinki.fi>
> To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
> Subject: [3dem] 4 PhD and postdoctoral postiions available in the
> University of Helsinki, Finland, DL 7th November 2024
> Message-ID:
> <
> HE1PR07MB4379F0A2BF4CF2F7BB1FDE60F44E2 at HE1PR07MB4379.eurprd07.prod.outlook.com
> >
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Dear Colleagues,
> My group, and those of Ville Paavilainen and Alex M?hleip are hiring both
> PhD and postdoctoral fellows for projects utilising SPA and tomography.
> Positions are 3-4 years in length.
> Please apply through the University of Helsinki portal where there are
> descriptions of the projects offered.
>
> https://urldefense.com/v3/__https://jobs.helsinki.fi/job/Helsinki-PostdoctoralGraduate-student-positions-in-structural-biologychemical-biologyvirology/804982202/?feedId=350602&utm_source=CareerSite_UniversityOfHelsinki__;!!Mih3wA!CFuJaEbJHLa1JPFLUFxvy1DNhdCR3Af2hge34t3bsgiXDkhHXMxZe8qmlLGY8dObn0yzp2x3aD4SCv6WWbQIDn-yf7WlTw$
> Deadline for applications: 7th November 2024
> Best regards,
> Sarah
>
> Prof. Sarah Butcher, Ph.D.
> Molecular & Integrative Biosciences Research Programme
> Faculty of Biological and Environmental Sciences
> &
> Helsinki Life Science Institute-Institute of Biotechnology
> room 2405
> P.O. Box 56 (Viikinkaari 9)
> FIN-00014 University of Helsinki
> Finland
> office: +358 2941 59563
> cell: +358 50 4155492
>
>
> https://urldefense.com/v3/__https://researchportal.helsinki.fi/en/persons/sarah-butcher__;!!Mih3wA!CFuJaEbJHLa1JPFLUFxvy1DNhdCR3Af2hge34t3bsgiXDkhHXMxZe8qmlLGY8dObn0yzp2x3aD4SCv6WWbQIDn9wMFmRmw$
>
> https://urldefense.com/v3/__https://blogs.helsinki.fi/butcher/__;!!Mih3wA!CFuJaEbJHLa1JPFLUFxvy1DNhdCR3Af2hge34t3bsgiXDkhHXMxZe8qmlLGY8dObn0yzp2x3aD4SCv6WWbQIDn_2hc2KAQ$
>
> https://urldefense.com/v3/__https://www.helsinki.fi/en/infrastructures/integrated-structural-cell-biology/cryoem__;!!Mih3wA!CFuJaEbJHLa1JPFLUFxvy1DNhdCR3Af2hge34t3bsgiXDkhHXMxZe8qmlLGY8dObn0yzp2x3aD4SCv6WWbQIDn8p8O3CvA$
>
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> ------------------------------
>
> Message: 2
> Date: Thu, 24 Oct 2024 13:55:36 +0000
> From: Ed Eng <eeng at nysbc.org>
> To: "CCPEM at JISCMAIL.AC.UK" <CCPEM at JISCMAIL.AC.UK>,
> "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
> Subject: [3dem] cryoEM staff microscopist position available - NYSBC,
> NYC, USA
> Message-ID:
> <
> BY5PR14MB34315D62D4EB8CF6B48A5CD5C24E2 at BY5PR14MB3431.namprd14.prod.outlook.com
> >
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Do you find operating cryoelectron microscopes everyday fun?
> Looking to work in NYC where the city's vibrant and diverse environment is
> reflected in its scientific community?
> Ready to start a career where you'll help researchers use cryoEM?
>
> Then join the SEMC Operations Team!
>
> We support cutting-edge scientific instrumentation enabling groundbreaking
> biomedical research. Be part of a dynamic team and contribute to advancing
> science every day.
>
> Find out more:
>
> *
>
> https://urldefense.com/v3/__https://jobrxiv.org/job/new-york-structural-biology-center-27778-staff-cryoelectron-microscopist/__;!!Mih3wA!DF1Yrcso5CI5wlKmClsMCXB_x3S7KsfHC_IVEDYNeDveyj_XD8bKmUSdDjFE08epLLOzI__5HV6zooA$
> *
>
> https://urldefense.com/v3/__https://www.indeed.com/job/staff-electron-microscopist-position-4ab0d33b3fd8951e__;!!Mih3wA!DF1Yrcso5CI5wlKmClsMCXB_x3S7KsfHC_IVEDYNeDveyj_XD8bKmUSdDjFE08epLLOzI__5svbqEMQ$
>
> Best,
> Ed
>
> ---
> Edward T. Eng, Ph.D.
> Senior Scientist, Manager
> New York Structural Biology Center
> 89 Convent Ave, NY, NY 10027
> nysbc.org | (212)939-0660 x344
>
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> ------------------------------
>
> Message: 3
> Date: Thu, 24 Oct 2024 10:41:26 -0400
> From: Liguo Wang <wanguw11 at gmail.com>
> To: 3dem at ncmir.ucsd.edu
> Subject: [3dem] LN2 Tank Handling for Aquilos 2 cryo-FIB
> Message-ID: <045c8097-f9ce-4e42-b4b0-dbbb6160fc2e at gmail.com>
> Content-Type: text/plain; charset=UTF-8; format=flowed
>
> Dear 3DEM community,
>
> We?ve been using the Aquilos 2 cryo-FIB and encountered a challenge with
> the LN2 tank. After daily use, a significant amount of LN2 remains, and
> we need to empty the tank and dry it using N2 gas. However, pouring the
> LN2 out of the tank is quite difficult and could potentially damage the
> plastic ring/cap.
>
> Does anyone have a better solution for safely and efficiently handling
> this?
>
> Thank you in advance for your help.
>
>
> Liguo Wang
> LBMS at BNL
>
>
>
> ------------------------------
>
> Message: 4
> Date: Thu, 24 Oct 2024 15:09:00 +0000
> From: Alex Cotten <cotten at wisc.edu>
> To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
> Subject: [3dem] Q4 2024 National Network for Cryo-Electron Tomography
> Application Deadline Extension
> Message-ID: <63E98555-69A2-47E3-8926-5A702ACDFAED at wisc.edu>
> Content-Type: text/plain; charset="utf-8"
>
> Dear 3dem Readers,
> We are excited to announce that the National Network for Cryo-Electron
> Tomography is currently accepting applications for its next round of
> research initiatives. As leading facilities equipped with stat
> state-of-the-art specimen preparation equipment and cryo-electron
> microscopes, we are committed to advancing electron tomography of
> biological specimens with nm to sub-nm imaging resolution.
>
> DEADLINE:
> Q4-2024 application cycle closes on **Friday, November 1, 2024**
>
> Eligibility: If you have a research initiative that aligns with
> cryo-electron tomography, we invite you to submit a detailed proposal
> through our application portal.
>
> https://urldefense.com/v3/__https://www.cryoetportal.org/__;!!Mih3wA!EfHbBiW1teGe5ZLdtgiitvJVC397udXuJfkQI2gNUbl5xZmrveX1pQ7rHUP1muHIWyLsTuKJZT9DRX2MGmg$
>
>
> Kind regards,
>
> -----------------
> Alex Cotten
> Research Center Administrator
> Cryo-Electron Microscopy Research Center
> Midwest Center for Cryo-Electron Tomography
> Morgridge Institute for Research
> University of Wisconsin-Madison
> cotten at wisc.edu<mailto:cotten at wisc.edu>
>
> https://urldefense.com/v3/__https://cryoem.wisc.edu__;!!Mih3wA!EfHbBiW1teGe5ZLdtgiitvJVC397udXuJfkQI2gNUbl5xZmrveX1pQ7rHUP1muHIWyLsTuKJZT9DpnJqYKw$
>
>
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> End of 3dem Digest, Vol 206, Issue 27
> *************************************
>
--
Qiang (John) Guo
Department of Molecular Structural Biology
Max Planck Institute of Biochemistry
Am Klopferspitz 18
82152 Martinsried
Germany
Email: guoq.tiger at gmail.com
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