<div dir="ltr"><div>Dear Liguo, </div><div><br></div><div>I believe you're referring to the gas cooling tank. If the microscope is used frequently, emptying the tank daily is unnecessary and wasteful. We typically refill it daily instead.<br><br>If emptying is essential in your lab, maybe consider a Polara-style setup: a long tube to the bottom, a lid that could seal the tank, and a heater.<br></div><div><br></div><div>Best,</div><div>Qiang</div><div><br></div>------------------------------------------<br>Message: 3<br>Date: Thu, 24 Oct 2024 10:41:26 -0400<br>From: Liguo Wang <<a href="mailto:wanguw11@gmail.com">wanguw11@gmail.com</a>><br>To: <a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a><br>Subject: [3dem] LN2 Tank Handling for Aquilos 2 cryo-FIB<br>Message-ID: <<a href="mailto:045c8097-f9ce-4e42-b4b0-dbbb6160fc2e@gmail.com">045c8097-f9ce-4e42-b4b0-dbbb6160fc2e@gmail.com</a>><br>Content-Type: text/plain; charset=UTF-8; format=flowed<br><br>Dear 3DEM community,<br><br>We?ve been using the Aquilos 2 cryo-FIB and encountered a challenge with<br>the LN2 tank. After daily use, a significant amount of LN2 remains, and<br>we need to empty the tank and dry it using N2 gas. However, pouring the<br>LN2 out of the tank is quite difficult and could potentially damage the<br>plastic ring/cap.<br><br>Does anyone have a better solution for safely and efficiently handling this?<br><br>Thank you in advance for your help.<br><br><br>Liguo Wang<br>LBMS at BNL<br></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr"><<a href="mailto:3dem-request@ncmir.ucsd.edu">3dem-request@ncmir.ucsd.edu</a>> 于2024年10月24日周四 23:25写道：<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">Send 3dem mailing list submissions to<br>
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Today's Topics:<br>
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1. 4 PhD and postdoctoral postiions available in the University<br>
of Helsinki, Finland, DL 7th November 2024 (Butcher, Sarah)<br>
2. cryoEM staff microscopist position available - NYSBC, NYC,<br>
USA (Ed Eng)<br>
3. LN2 Tank Handling for Aquilos 2 cryo-FIB (Liguo Wang)<br>
4. Q4 2024 National Network for Cryo-Electron Tomography<br>
Application Deadline Extension (Alex Cotten)<br>
<br>
<br>
----------------------------------------------------------------------<br>
<br>
Message: 1<br>
Date: Thu, 24 Oct 2024 02:06:17 +0000<br>
From: "Butcher, Sarah" <<a href="mailto:sarah.butcher@helsinki.fi" target="_blank">sarah.butcher@helsinki.fi</a>><br>
To: "<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a>" <<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a>><br>
Subject: [3dem] 4 PhD and postdoctoral postiions available in the<br>
University of Helsinki, Finland, DL 7th November 2024<br>
Message-ID:<br>
<<a href="mailto:HE1PR07MB4379F0A2BF4CF2F7BB1FDE60F44E2@HE1PR07MB4379.eurprd07.prod.outlook.com" target="_blank">HE1PR07MB4379F0A2BF4CF2F7BB1FDE60F44E2@HE1PR07MB4379.eurprd07.prod.outlook.com</a>><br>
<br>
Content-Type: text/plain; charset="iso-8859-1"<br>
<br>
Dear Colleagues,<br>
My group, and those of Ville Paavilainen and Alex M?hleip are hiring both PhD and postdoctoral fellows for projects utilising SPA and tomography. Positions are 3-4 years in length.<br>
Please apply through the University of Helsinki portal where there are descriptions of the projects offered.<br>
<a href="https://urldefense.com/v3/__https://jobs.helsinki.fi/job/Helsinki-PostdoctoralGraduate-student-positions-in-structural-biologychemical-biologyvirology/804982202/?feedId=350602&utm_source=CareerSite_UniversityOfHelsinki__;!!Mih3wA!CFuJaEbJHLa1JPFLUFxvy1DNhdCR3Af2hge34t3bsgiXDkhHXMxZe8qmlLGY8dObn0yzp2x3aD4SCv6WWbQIDn-yf7WlTw$" rel="noreferrer" target="_blank">https://urldefense.com/v3/__https://jobs.helsinki.fi/job/Helsinki-PostdoctoralGraduate-student-positions-in-structural-biologychemical-biologyvirology/804982202/?feedId=350602&utm_source=CareerSite_UniversityOfHelsinki__;!!Mih3wA!CFuJaEbJHLa1JPFLUFxvy1DNhdCR3Af2hge34t3bsgiXDkhHXMxZe8qmlLGY8dObn0yzp2x3aD4SCv6WWbQIDn-yf7WlTw$</a> <br>
Deadline for applications: 7th November 2024<br>
Best regards,<br>
Sarah<br>
<br>
Prof. Sarah Butcher, Ph.D.<br>
Molecular & Integrative Biosciences Research Programme<br>
Faculty of Biological and Environmental Sciences<br>
&<br>
Helsinki Life Science Institute-Institute of Biotechnology<br>
room 2405<br>
P.O. Box 56 (Viikinkaari 9)<br>
FIN-00014 University of Helsinki<br>
Finland<br>
office: +358 2941 59563<br>
cell: +358 50 4155492<br>
<br>
<a href="https://urldefense.com/v3/__https://researchportal.helsinki.fi/en/persons/sarah-butcher__;!!Mih3wA!CFuJaEbJHLa1JPFLUFxvy1DNhdCR3Af2hge34t3bsgiXDkhHXMxZe8qmlLGY8dObn0yzp2x3aD4SCv6WWbQIDn9wMFmRmw$" rel="noreferrer" target="_blank">https://urldefense.com/v3/__https://researchportal.helsinki.fi/en/persons/sarah-butcher__;!!Mih3wA!CFuJaEbJHLa1JPFLUFxvy1DNhdCR3Af2hge34t3bsgiXDkhHXMxZe8qmlLGY8dObn0yzp2x3aD4SCv6WWbQIDn9wMFmRmw$</a> <br>
<a href="https://urldefense.com/v3/__https://blogs.helsinki.fi/butcher/__;!!Mih3wA!CFuJaEbJHLa1JPFLUFxvy1DNhdCR3Af2hge34t3bsgiXDkhHXMxZe8qmlLGY8dObn0yzp2x3aD4SCv6WWbQIDn_2hc2KAQ$" rel="noreferrer" target="_blank">https://urldefense.com/v3/__https://blogs.helsinki.fi/butcher/__;!!Mih3wA!CFuJaEbJHLa1JPFLUFxvy1DNhdCR3Af2hge34t3bsgiXDkhHXMxZe8qmlLGY8dObn0yzp2x3aD4SCv6WWbQIDn_2hc2KAQ$</a> <br>
<a href="https://urldefense.com/v3/__https://www.helsinki.fi/en/infrastructures/integrated-structural-cell-biology/cryoem__;!!Mih3wA!CFuJaEbJHLa1JPFLUFxvy1DNhdCR3Af2hge34t3bsgiXDkhHXMxZe8qmlLGY8dObn0yzp2x3aD4SCv6WWbQIDn8p8O3CvA$" rel="noreferrer" target="_blank">https://urldefense.com/v3/__https://www.helsinki.fi/en/infrastructures/integrated-structural-cell-biology/cryoem__;!!Mih3wA!CFuJaEbJHLa1JPFLUFxvy1DNhdCR3Af2hge34t3bsgiXDkhHXMxZe8qmlLGY8dObn0yzp2x3aD4SCv6WWbQIDn8p8O3CvA$</a> <br>
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Message: 2<br>
Date: Thu, 24 Oct 2024 13:55:36 +0000<br>
From: Ed Eng <<a href="mailto:eeng@nysbc.org" target="_blank">eeng@nysbc.org</a>><br>
To: "<a href="mailto:CCPEM@JISCMAIL.AC.UK" target="_blank">CCPEM@JISCMAIL.AC.UK</a>" <<a href="mailto:CCPEM@JISCMAIL.AC.UK" target="_blank">CCPEM@JISCMAIL.AC.UK</a>>,<br>
"<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a>" <<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a>><br>
Subject: [3dem] cryoEM staff microscopist position available - NYSBC,<br>
NYC, USA<br>
Message-ID:<br>
<<a href="mailto:BY5PR14MB34315D62D4EB8CF6B48A5CD5C24E2@BY5PR14MB3431.namprd14.prod.outlook.com" target="_blank">BY5PR14MB34315D62D4EB8CF6B48A5CD5C24E2@BY5PR14MB3431.namprd14.prod.outlook.com</a>><br>
<br>
Content-Type: text/plain; charset="iso-8859-1"<br>
<br>
Do you find operating cryoelectron microscopes everyday fun?<br>
Looking to work in NYC where the city's vibrant and diverse environment is reflected in its scientific community?<br>
Ready to start a career where you'll help researchers use cryoEM?<br>
<br>
Then join the SEMC Operations Team!<br>
<br>
We support cutting-edge scientific instrumentation enabling groundbreaking biomedical research. Be part of a dynamic team and contribute to advancing science every day.<br>
<br>
Find out more:<br>
<br>
*<br>
<a href="https://urldefense.com/v3/__https://jobrxiv.org/job/new-york-structural-biology-center-27778-staff-cryoelectron-microscopist/__;!!Mih3wA!DF1Yrcso5CI5wlKmClsMCXB_x3S7KsfHC_IVEDYNeDveyj_XD8bKmUSdDjFE08epLLOzI__5HV6zooA$" rel="noreferrer" target="_blank">https://urldefense.com/v3/__https://jobrxiv.org/job/new-york-structural-biology-center-27778-staff-cryoelectron-microscopist/__;!!Mih3wA!DF1Yrcso5CI5wlKmClsMCXB_x3S7KsfHC_IVEDYNeDveyj_XD8bKmUSdDjFE08epLLOzI__5HV6zooA$</a> <br>
*<br>
<a href="https://urldefense.com/v3/__https://www.indeed.com/job/staff-electron-microscopist-position-4ab0d33b3fd8951e__;!!Mih3wA!DF1Yrcso5CI5wlKmClsMCXB_x3S7KsfHC_IVEDYNeDveyj_XD8bKmUSdDjFE08epLLOzI__5svbqEMQ$" rel="noreferrer" target="_blank">https://urldefense.com/v3/__https://www.indeed.com/job/staff-electron-microscopist-position-4ab0d33b3fd8951e__;!!Mih3wA!DF1Yrcso5CI5wlKmClsMCXB_x3S7KsfHC_IVEDYNeDveyj_XD8bKmUSdDjFE08epLLOzI__5svbqEMQ$</a> <br>
<br>
Best,<br>
Ed<br>
<br>
---<br>
Edward T. Eng, Ph.D.<br>
Senior Scientist, Manager<br>
New York Structural Biology Center<br>
89 Convent Ave, NY, NY 10027<br>
<a href="https://urldefense.com/v3/__http://nysbc.org__;!!Mih3wA!BY5Dfc-qNE_3F7imASQM3ckqe1YdJPBJXyrpEVdA3SOYW84txuxyXCUg1weeptWfSIxdKuj3EMiflw1ZA7TtsA$" rel="noreferrer" target="_blank">nysbc.org</a> | (212)939-0660 x344<br>
<br>
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Message: 3<br>
Date: Thu, 24 Oct 2024 10:41:26 -0400<br>
From: Liguo Wang <<a href="mailto:wanguw11@gmail.com" target="_blank">wanguw11@gmail.com</a>><br>
To: <a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a><br>
Subject: [3dem] LN2 Tank Handling for Aquilos 2 cryo-FIB<br>
Message-ID: <<a href="mailto:045c8097-f9ce-4e42-b4b0-dbbb6160fc2e@gmail.com" target="_blank">045c8097-f9ce-4e42-b4b0-dbbb6160fc2e@gmail.com</a>><br>
Content-Type: text/plain; charset=UTF-8; format=flowed<br>
<br>
Dear 3DEM community,<br>
<br>
We?ve been using the Aquilos 2 cryo-FIB and encountered a challenge with <br>
the LN2 tank. After daily use, a significant amount of LN2 remains, and <br>
we need to empty the tank and dry it using N2 gas. However, pouring the <br>
LN2 out of the tank is quite difficult and could potentially damage the <br>
plastic ring/cap.<br>
<br>
Does anyone have a better solution for safely and efficiently handling this?<br>
<br>
Thank you in advance for your help.<br>
<br>
<br>
Liguo Wang<br>
LBMS at BNL<br>
<br>
<br>
<br>
------------------------------<br>
<br>
Message: 4<br>
Date: Thu, 24 Oct 2024 15:09:00 +0000<br>
From: Alex Cotten <<a href="mailto:cotten@wisc.edu" target="_blank">cotten@wisc.edu</a>><br>
To: "<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a>" <<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a>><br>
Subject: [3dem] Q4 2024 National Network for Cryo-Electron Tomography<br>
Application Deadline Extension<br>
Message-ID: <<a href="mailto:63E98555-69A2-47E3-8926-5A702ACDFAED@wisc.edu" target="_blank">63E98555-69A2-47E3-8926-5A702ACDFAED@wisc.edu</a>><br>
Content-Type: text/plain; charset="utf-8"<br>
<br>
Dear 3dem Readers,<br>
We are excited to announce that the National Network for Cryo-Electron Tomography is currently accepting applications for its next round of research initiatives. As leading facilities equipped with stat state-of-the-art specimen preparation equipment and cryo-electron microscopes, we are committed to advancing electron tomography of biological specimens with nm to sub-nm imaging resolution.<br>
<br>
DEADLINE:<br>
Q4-2024 application cycle closes on **Friday, November 1, 2024**<br>
<br>
Eligibility: If you have a research initiative that aligns with cryo-electron tomography, we invite you to submit a detailed proposal through our application portal.<br>
<a href="https://urldefense.com/v3/__https://www.cryoetportal.org/__;!!Mih3wA!EfHbBiW1teGe5ZLdtgiitvJVC397udXuJfkQI2gNUbl5xZmrveX1pQ7rHUP1muHIWyLsTuKJZT9DRX2MGmg$" rel="noreferrer" target="_blank">https://urldefense.com/v3/__https://www.cryoetportal.org/__;!!Mih3wA!EfHbBiW1teGe5ZLdtgiitvJVC397udXuJfkQI2gNUbl5xZmrveX1pQ7rHUP1muHIWyLsTuKJZT9DRX2MGmg$</a> <br>
<br>
<br>
Kind regards,<br>
<br>
-----------------<br>
Alex Cotten<br>
Research Center Administrator<br>
Cryo-Electron Microscopy Research Center<br>
Midwest Center for Cryo-Electron Tomography<br>
Morgridge Institute for Research<br>
University of Wisconsin-Madison<br>
<a href="mailto:cotten@wisc.edu" target="_blank">cotten@wisc.edu</a><mailto:<a href="mailto:cotten@wisc.edu" target="_blank">cotten@wisc.edu</a>><br>
<a href="https://urldefense.com/v3/__https://cryoem.wisc.edu__;!!Mih3wA!EfHbBiW1teGe5ZLdtgiitvJVC397udXuJfkQI2gNUbl5xZmrveX1pQ7rHUP1muHIWyLsTuKJZT9DpnJqYKw$" rel="noreferrer" target="_blank">https://urldefense.com/v3/__https://cryoem.wisc.edu__;!!Mih3wA!EfHbBiW1teGe5ZLdtgiitvJVC397udXuJfkQI2gNUbl5xZmrveX1pQ7rHUP1muHIWyLsTuKJZT9DpnJqYKw$</a> <br>
<br>
<br>
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</blockquote></div><br clear="all"><div><br></div><span class="gmail_signature_prefix">-- </span><br><div dir="ltr" class="gmail_signature"><div dir="ltr">Qiang (John) Guo<br>Department of Molecular Structural Biology<br>Max Planck Institute of Biochemistry<br>Am Klopferspitz 18<br>82152 Martinsried<br>Germany<br>Email: <a href="mailto:guoq.tiger@gmail.com" target="_blank">guoq.tiger@gmail.com</a> <br></div></div>