[3dem] 3dem Digest, Vol 208, Issue 18

Tue Dec 31 07:10:01 PST 2024

Hi Ashraf,

Just to clarify on the "autofill" point, both the Nanosoft and SubAngstrom
systems are not autofill, but rather larger dewars (plus some additional
modifications to the cooling system) that allow extended operation without
the need to fill the dewar for ~1 week.  The Norhof system that Michael
shared is *truly* an autofill system.  That's pretty cool and I didn't even
know it existed - thanks for sharing, Michael!  Practically speaking,
though, I'd say the two alternatives are about the same.  In both cases
you'll probably need to change a tank roughly once per week.

We have the Nanosoft system and are quite happy with it.  We've run it
continuously for ~2 weeks+ and never gotten a clog.  The reason we chose
Nanosoft over SubAngstrom is that they are more transparent about pricing,
but I believe the two systems work equally well.

Feel free to email me if you have more questions,
David

On Mon, Dec 30, 2024 at 12:00 PM <3dem-request at ncmir.ucsd.edu> wrote:

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> Today's Topics:
>
>    1. Autofill for Cryo-FIB Machine (Ashraf Alamoudi)
>    2. Re: Autofill for Cryo-FIB Machine (Michael Elbaum)
>    3. Re: Autofill for Cryo-FIB Machine (Zongli Li)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Mon, 30 Dec 2024 10:22:05 +0000
> From: Ashraf Alamoudi <ashraf.alamoudi at kaust.edu.sa>
> To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
> Subject: [3dem] Autofill for Cryo-FIB Machine
> Message-ID: <66A75398-773A-4A64-87A9-780874EAFFA3 at contoso.com>
> Content-Type: text/plain; charset="utf-8"
>
> Dear all,
>
> We have recently acquired the Cryo PFIB Hydra Bio and are now seeking an
> automatic LN2 filling system. I understand that SubAng offers such a
> system, and I would appreciate it if you could share your insights or
> experiences with this system, or with any other available options on the
> market.
>
> Thank you.
>
> All the best,
>
> Ashraf
>
> --
> Ashraf Al-Amoudi, PhD
> Senior Staff Scientist
> Electron Microscopy Lab
> Imaging and Characterization Core Labs
> King Abdullah University of Science and Technology (KAUST)
> Bld 3 ? L 0 ? Office 0239
> Thuwal 23955-6900
> Kingdom of Saudi Arabia
>
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> ------------------------------
>
> Message: 2
> Date: Mon, 30 Dec 2024 10:40:45 +0000
> From: Michael Elbaum <michael.elbaum at weizmann.ac.il>
> To: Ashraf Alamoudi <ashraf.alamoudi at kaust.edu.sa>,
>         "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
> Subject: Re: [3dem] Autofill for Cryo-FIB Machine
> Message-ID:
>         <
> PA4P191MB147090C20B1A07444CDC8296AB092 at PA4P191MB1470.EURP191.PROD.OUTLOOK.COM
> >
>
> Content-Type: text/plain; charset="windows-1252"
>
> Hi Ashraf,     Norhof makes an automatic filler that we use on a number of
> instruments.
> https://urldefense.com/v3/__https://www.norhof.com/__;!!Mih3wA!GFYlGLBrZm8gAgiJ6XpIinhUM6pGkEsCUh3vge0CvoT42UTEuISV7WWNA2AxJrVRPukK_e9uYnYWhxXzVj5hTUSoXx146q0Lqg$
>  <
> https://urldefense.com/v3/__https://www.norhof.com/__;!!Mih3wA!GFYlGLBrZm8gAgiJ6XpIinhUM6pGkEsCUh3vge0CvoT42UTEuISV7WWNA2AxJrVRPukK_e9uYnYWhxXzVj5hTUSoXx146q0Lqg$
> >  It's much more convenient than filling by hand.
> best wishes,
> Michael
> ________________________________
> From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Ashraf Alamoudi <
> ashraf.alamoudi at kaust.edu.sa>
> Sent: Monday, December 30, 2024 12:22
> To: 3dem at ncmir.ucsd.edu <3dem at ncmir.ucsd.edu>
> Subject: [3dem] Autofill for Cryo-FIB Machine
>
> Caution: External Sender. Do not click on links or open attachments unless
> you recognize the sender.
>
>
> Dear all,
>
>
>
> We have recently acquired the Cryo PFIB Hydra Bio and are now seeking an
> automatic LN2 filling system. I understand that SubAng offers such a
> system, and I would appreciate it if you could share your insights or
> experiences with this system, or with any other available options on the
> market.
>
>
>
> Thank you.
>
>
>
> All the best,
>
>
>
> Ashraf
>
>
>
> --
>
> Ashraf Al-Amoudi, PhD
>
> Senior Staff Scientist
>
> Electron Microscopy Lab
>
> Imaging and Characterization Core Labs
>
> King Abdullah University of Science and Technology (KAUST)
>
> Bld 3 ? L 0 ? Office 0239
>
> Thuwal 23955-6900
>
> Kingdom of Saudi Arabia
>
>
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>
> ------------------------------
>
> Message: 3
> Date: Mon, 30 Dec 2024 10:52:53 -0500
> From: Zongli Li <zongli.li at gmail.com>
> To: Michael Elbaum <michael.elbaum at weizmann.ac.il>
> Cc: Ashraf Alamoudi <ashraf.alamoudi at kaust.edu.sa>,
>         "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
> Subject: Re: [3dem] Autofill for Cryo-FIB Machine
> Message-ID:
>         <
> CAPWpDO2++CxsF5NWqtfHjMtLQ22i5h-JHo60D2wEt-_qzxZ+sw at mail.gmail.com>
> Content-Type: text/plain; charset="utf-8"
>
> Hi Ashraf,
> We have been using the LN2 auto-filling system from Nanosoft and it works
> pretty well.
>
> https://urldefense.com/v3/__http://www.nanosoftmaterials.com__;!!Mih3wA!CvhnJ2jBagGTJD3yhBfzHFmSRtjNjzgGJrVpfhsWlg_D1GTdVH5DEZnN6osUYzOWoa6ItvOYAhY4LWP3SBOr$
>
> Best,
>
> Zongli
>
> On Mon, Dec 30, 2024 at 5:41?AM Michael Elbaum <
> michael.elbaum at weizmann.ac.il> wrote:
>
> > Hi Ashraf,     Norhof makes an automatic filler that we use on a number
> of
> > instruments.
> https://urldefense.com/v3/__https://www.norhof.com/__;!!Mih3wA!CvhnJ2jBagGTJD3yhBfzHFmSRtjNjzgGJrVpfhsWlg_D1GTdVH5DEZnN6osUYzOWoa6ItvOYAhY4LYtsUGi6$
> > <
> https://urldefense.com/v3/__https://www.norhof.com/__;!!Mih3wA!GFYlGLBrZm8gAgiJ6XpIinhUM6pGkEsCUh3vge0CvoT42UTEuISV7WWNA2AxJrVRPukK_e9uYnYWhxXzVj5hTUSoXx146q0Lqg$>
> It's
> > much more convenient than filling by hand.
> > best wishes,
> > Michael
> > ------------------------------
> > *From:* 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Ashraf Alamoudi
> <
> > ashraf.alamoudi at kaust.edu.sa>
> > *Sent:* Monday, December 30, 2024 12:22
> > *To:* 3dem at ncmir.ucsd.edu <3dem at ncmir.ucsd.edu>
> > *Subject:* [3dem] Autofill for Cryo-FIB Machine
> >
> > *Caution: External Sender. Do not click on links or open attachments
> > unless you recognize the sender.*
> >
> >
> > Dear all,
> >
> >
> >
> > We have recently acquired the Cryo PFIB Hydra Bio and are now seeking an
> > automatic LN2 filling system. I understand that SubAng offers such a
> > system, and I would appreciate it if you could share your insights or
> > experiences with this system, or with any other available options on the
> > market.
> >
> >
> >
> > Thank you.
> >
> >
> >
> > All the best,
> >
> >
> >
> > Ashraf
> >
> >
> >
> > --
> >
> > Ashraf Al-Amoudi, PhD
> >
> > Senior Staff Scientist
> >
> > Electron Microscopy Lab
> >
> > Imaging and Characterization Core Labs
> >
> > King Abdullah University of Science and Technology (KAUST)
> >
> > Bld 3 ? L 0 ? Office 0239
> >
> > Thuwal 23955-6900
> >
> > Kingdom of Saudi Arabia
> >
> >
> > _______________________________________________
> > 3dem mailing list
> > 3dem at ncmir.ucsd.edu
> > https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
> >
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> End of 3dem Digest, Vol 208, Issue 18
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