<div dir="ltr"><div>Hi Ashraf,</div><div><br></div><div>Just to clarify on the "autofill" point, both the Nanosoft and SubAngstrom systems are not autofill, but rather larger dewars (plus some additional modifications to the cooling system) that allow extended operation without the need to fill the dewar for ~1 week. The Norhof system that Michael shared is *truly* an autofill system. That's pretty cool and I didn't even know it existed - thanks for sharing, Michael! Practically speaking, though, I'd say the two alternatives are about the same. In both cases you'll probably need to change a tank roughly once per week.<br></div><div><br></div><div>We have the Nanosoft system and are quite happy with it. We've run it continuously for ~2 weeks+ and never gotten a clog. The reason we chose Nanosoft over SubAngstrom is that they are more transparent about pricing, but I believe the two systems work equally well.</div><div><br></div><div>Feel free to email me if you have more questions,</div><div>David<br></div><div><br></div></div><br><div class="gmail_quote gmail_quote_container"><div dir="ltr" class="gmail_attr">On Mon, Dec 30, 2024 at 12:00 PM <<a href="mailto:3dem-request@ncmir.ucsd.edu">3dem-request@ncmir.ucsd.edu</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">Send 3dem mailing list submissions to<br>
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Today's Topics:<br>
<br>
1. Autofill for Cryo-FIB Machine (Ashraf Alamoudi)<br>
2. Re: Autofill for Cryo-FIB Machine (Michael Elbaum)<br>
3. Re: Autofill for Cryo-FIB Machine (Zongli Li)<br>
<br>
<br>
----------------------------------------------------------------------<br>
<br>
Message: 1<br>
Date: Mon, 30 Dec 2024 10:22:05 +0000<br>
From: Ashraf Alamoudi <<a href="mailto:ashraf.alamoudi@kaust.edu.sa" target="_blank">ashraf.alamoudi@kaust.edu.sa</a>><br>
To: "<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a>" <<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a>><br>
Subject: [3dem] Autofill for Cryo-FIB Machine<br>
Message-ID: <<a href="mailto:66A75398-773A-4A64-87A9-780874EAFFA3@contoso.com" target="_blank">66A75398-773A-4A64-87A9-780874EAFFA3@contoso.com</a>><br>
Content-Type: text/plain; charset="utf-8"<br>
<br>
Dear all,<br>
<br>
We have recently acquired the Cryo PFIB Hydra Bio and are now seeking an automatic LN2 filling system. I understand that SubAng offers such a system, and I would appreciate it if you could share your insights or experiences with this system, or with any other available options on the market.<br>
<br>
Thank you.<br>
<br>
All the best,<br>
<br>
Ashraf<br>
<br>
--<br>
Ashraf Al-Amoudi, PhD<br>
Senior Staff Scientist<br>
Electron Microscopy Lab<br>
Imaging and Characterization Core Labs<br>
King Abdullah University of Science and Technology (KAUST)<br>
Bld 3 ? L 0 ? Office 0239<br>
Thuwal 23955-6900<br>
Kingdom of Saudi Arabia<br>
<br>
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Message: 2<br>
Date: Mon, 30 Dec 2024 10:40:45 +0000<br>
From: Michael Elbaum <<a href="mailto:michael.elbaum@weizmann.ac.il" target="_blank">michael.elbaum@weizmann.ac.il</a>><br>
To: Ashraf Alamoudi <<a href="mailto:ashraf.alamoudi@kaust.edu.sa" target="_blank">ashraf.alamoudi@kaust.edu.sa</a>>,<br>
"<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a>" <<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a>><br>
Subject: Re: [3dem] Autofill for Cryo-FIB Machine<br>
Message-ID:<br>
<<a href="mailto:PA4P191MB147090C20B1A07444CDC8296AB092@PA4P191MB1470.EURP191.PROD.OUTLOOK.COM" target="_blank">PA4P191MB147090C20B1A07444CDC8296AB092@PA4P191MB1470.EURP191.PROD.OUTLOOK.COM</a>><br>
<br>
Content-Type: text/plain; charset="windows-1252"<br>
<br>
Hi Ashraf, Norhof makes an automatic filler that we use on a number of instruments. <a href="https://urldefense.com/v3/__https://www.norhof.com/__;!!Mih3wA!GFYlGLBrZm8gAgiJ6XpIinhUM6pGkEsCUh3vge0CvoT42UTEuISV7WWNA2AxJrVRPukK_e9uYnYWhxXzVj5hTUSoXx146q0Lqg$" rel="noreferrer" target="_blank">https://urldefense.com/v3/__https://www.norhof.com/__;!!Mih3wA!GFYlGLBrZm8gAgiJ6XpIinhUM6pGkEsCUh3vge0CvoT42UTEuISV7WWNA2AxJrVRPukK_e9uYnYWhxXzVj5hTUSoXx146q0Lqg$</a> <<a href="https://urldefense.com/v3/__https://www.norhof.com/__;!!Mih3wA!GFYlGLBrZm8gAgiJ6XpIinhUM6pGkEsCUh3vge0CvoT42UTEuISV7WWNA2AxJrVRPukK_e9uYnYWhxXzVj5hTUSoXx146q0Lqg$" rel="noreferrer" target="_blank">https://urldefense.com/v3/__https://www.norhof.com/__;!!Mih3wA!GFYlGLBrZm8gAgiJ6XpIinhUM6pGkEsCUh3vge0CvoT42UTEuISV7WWNA2AxJrVRPukK_e9uYnYWhxXzVj5hTUSoXx146q0Lqg$</a> > It's much more convenient than filling by hand.<br>
best wishes,<br>
Michael<br>
________________________________<br>
From: 3dem <<a href="mailto:3dem-bounces@ncmir.ucsd.edu" target="_blank">3dem-bounces@ncmir.ucsd.edu</a>> on behalf of Ashraf Alamoudi <<a href="mailto:ashraf.alamoudi@kaust.edu.sa" target="_blank">ashraf.alamoudi@kaust.edu.sa</a>><br>
Sent: Monday, December 30, 2024 12:22<br>
To: <a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a> <<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a>><br>
Subject: [3dem] Autofill for Cryo-FIB Machine<br>
<br>
Caution: External Sender. Do not click on links or open attachments unless you recognize the sender.<br>
<br>
<br>
Dear all,<br>
<br>
<br>
<br>
We have recently acquired the Cryo PFIB Hydra Bio and are now seeking an automatic LN2 filling system. I understand that SubAng offers such a system, and I would appreciate it if you could share your insights or experiences with this system, or with any other available options on the market.<br>
<br>
<br>
<br>
Thank you.<br>
<br>
<br>
<br>
All the best,<br>
<br>
<br>
<br>
Ashraf<br>
<br>
<br>
<br>
--<br>
<br>
Ashraf Al-Amoudi, PhD<br>
<br>
Senior Staff Scientist<br>
<br>
Electron Microscopy Lab<br>
<br>
Imaging and Characterization Core Labs<br>
<br>
King Abdullah University of Science and Technology (KAUST)<br>
<br>
Bld 3 ? L 0 ? Office 0239<br>
<br>
Thuwal 23955-6900<br>
<br>
Kingdom of Saudi Arabia<br>
<br>
<br>
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<br>
Message: 3<br>
Date: Mon, 30 Dec 2024 10:52:53 -0500<br>
From: Zongli Li <<a href="mailto:zongli.li@gmail.com" target="_blank">zongli.li@gmail.com</a>><br>
To: Michael Elbaum <<a href="mailto:michael.elbaum@weizmann.ac.il" target="_blank">michael.elbaum@weizmann.ac.il</a>><br>
Cc: Ashraf Alamoudi <<a href="mailto:ashraf.alamoudi@kaust.edu.sa" target="_blank">ashraf.alamoudi@kaust.edu.sa</a>>,<br>
"<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a>" <<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a>><br>
Subject: Re: [3dem] Autofill for Cryo-FIB Machine<br>
Message-ID:<br>
<<a href="mailto:CAPWpDO2%2B%2BCxsF5NWqtfHjMtLQ22i5h-JHo60D2wEt-_qzxZ%2Bsw@mail.gmail.com" target="_blank">CAPWpDO2++CxsF5NWqtfHjMtLQ22i5h-JHo60D2wEt-_qzxZ+sw@mail.gmail.com</a>><br>
Content-Type: text/plain; charset="utf-8"<br>
<br>
Hi Ashraf,<br>
We have been using the LN2 auto-filling system from Nanosoft and it works<br>
pretty well.<br>
<a href="https://urldefense.com/v3/__http://www.nanosoftmaterials.com__;!!Mih3wA!CvhnJ2jBagGTJD3yhBfzHFmSRtjNjzgGJrVpfhsWlg_D1GTdVH5DEZnN6osUYzOWoa6ItvOYAhY4LWP3SBOr$" rel="noreferrer" target="_blank">https://urldefense.com/v3/__http://www.nanosoftmaterials.com__;!!Mih3wA!CvhnJ2jBagGTJD3yhBfzHFmSRtjNjzgGJrVpfhsWlg_D1GTdVH5DEZnN6osUYzOWoa6ItvOYAhY4LWP3SBOr$</a> <br>
<br>
Best,<br>
<br>
Zongli<br>
<br>
On Mon, Dec 30, 2024 at 5:41?AM Michael Elbaum <<br>
<a href="mailto:michael.elbaum@weizmann.ac.il" target="_blank">michael.elbaum@weizmann.ac.il</a>> wrote:<br>
<br>
> Hi Ashraf, Norhof makes an automatic filler that we use on a number of<br>
> instruments. <a href="https://urldefense.com/v3/__https://www.norhof.com/__;!!Mih3wA!CvhnJ2jBagGTJD3yhBfzHFmSRtjNjzgGJrVpfhsWlg_D1GTdVH5DEZnN6osUYzOWoa6ItvOYAhY4LYtsUGi6$" rel="noreferrer" target="_blank">https://urldefense.com/v3/__https://www.norhof.com/__;!!Mih3wA!CvhnJ2jBagGTJD3yhBfzHFmSRtjNjzgGJrVpfhsWlg_D1GTdVH5DEZnN6osUYzOWoa6ItvOYAhY4LYtsUGi6$</a> <br>
> <<a href="https://urldefense.com/v3/__https://www.norhof.com/__;!!Mih3wA!GFYlGLBrZm8gAgiJ6XpIinhUM6pGkEsCUh3vge0CvoT42UTEuISV7WWNA2AxJrVRPukK_e9uYnYWhxXzVj5hTUSoXx146q0Lqg$" rel="noreferrer" target="_blank">https://urldefense.com/v3/__https://www.norhof.com/__;!!Mih3wA!GFYlGLBrZm8gAgiJ6XpIinhUM6pGkEsCUh3vge0CvoT42UTEuISV7WWNA2AxJrVRPukK_e9uYnYWhxXzVj5hTUSoXx146q0Lqg$</a>> It's<br>
> much more convenient than filling by hand.<br>
> best wishes,<br>
> Michael<br>
> ------------------------------<br>
> *From:* 3dem <<a href="mailto:3dem-bounces@ncmir.ucsd.edu" target="_blank">3dem-bounces@ncmir.ucsd.edu</a>> on behalf of Ashraf Alamoudi <<br>
> <a href="mailto:ashraf.alamoudi@kaust.edu.sa" target="_blank">ashraf.alamoudi@kaust.edu.sa</a>><br>
> *Sent:* Monday, December 30, 2024 12:22<br>
> *To:* <a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a> <<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a>><br>
> *Subject:* [3dem] Autofill for Cryo-FIB Machine<br>
><br>
> *Caution: External Sender. Do not click on links or open attachments<br>
> unless you recognize the sender.*<br>
><br>
><br>
> Dear all,<br>
><br>
><br>
><br>
> We have recently acquired the Cryo PFIB Hydra Bio and are now seeking an<br>
> automatic LN2 filling system. I understand that SubAng offers such a<br>
> system, and I would appreciate it if you could share your insights or<br>
> experiences with this system, or with any other available options on the<br>
> market.<br>
><br>
><br>
><br>
> Thank you.<br>
><br>
><br>
><br>
> All the best,<br>
><br>
><br>
><br>
> Ashraf<br>
><br>
><br>
><br>
> --<br>
><br>
> Ashraf Al-Amoudi, PhD<br>
><br>
> Senior Staff Scientist<br>
><br>
> Electron Microscopy Lab<br>
><br>
> Imaging and Characterization Core Labs<br>
><br>
> King Abdullah University of Science and Technology (KAUST)<br>
><br>
> Bld 3 ? L 0 ? Office 0239<br>
><br>
> Thuwal 23955-6900<br>
><br>
> Kingdom of Saudi Arabia<br>
><br>
><br>
> _______________________________________________<br>
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