[3dem] Recent CryoSPARC videos and tutorials

[Ali Punjani]

Dear 3DEM,

Please see below for a roundup of CryoSPARC and cryo-EM data processing
educational materials released over the past few months:

   - Tutorial Videos: Recordings of the CryoSPARC tutorials
   <https://urldefense.com/v3/__https://www.youtube.com/playlist?list=PL39mLm0042zJk_ZkM2_OaIoL78uYj6JAb__;!!Mih3wA!H-jDVE5dOSqlt_TvkonWuvsFdT5in_A8JGf6rgj35_X-948EsclVqheOVpT8K9vOty8RzHMdfeHKSlQA5QwjLiTN$ >
   from this year's Stanford-SLAC CryoEM Center (S2C2) Image Processing
   Workshop are available. Over the course of six videos, we delve into
   cryo-EM fundamentals, a standard workflow for processing SPA datasets,
   considerations for working with symmetric samples, and ways to deal with
   discrete and continuous heterogeneity, using four EMPIAR datasets as
   examples. We are very grateful for S2C2's invitation and encourage users to
   check out other speakers' talks on S2C2's website
   <https://urldefense.com/v3/__https://s2c2.slac.stanford.edu/training/workshops-lectures/workshop-lecture-archive__;!!Mih3wA!H-jDVE5dOSqlt_TvkonWuvsFdT5in_A8JGf6rgj35_X-948EsclVqheOVpT8K9vOty8RzHMdfeHKSlQA5RMklgA4$ >
   .
   - Case Study: End to end processing of encapsulin and encapsulated
   ferritin
   <https://urldefense.com/v3/__https://guide.cryosparc.com/processing-data/tutorials-and-case-studies/case-study-end-to-end-processing-of-encapsulated-ferritin-empiar-10716__;!!Mih3wA!H-jDVE5dOSqlt_TvkonWuvsFdT5in_A8JGf6rgj35_X-948EsclVqheOVpT8K9vOty8RzHMdfeHKSlQA5REldEfF$ >
   (EMPIAR-10716), with a focus on challenges and strategies for handling
   mixed high-order and non-point-group symmetries.
   - Updated Guide Pages - CTF Estimation: We have released updated guide
   pages for CTF Estimation (theory)
   <https://urldefense.com/v3/__https://guide.cryosparc.com/processing-data/all-job-types-in-cryosparc/ctf-estimation__;!!Mih3wA!H-jDVE5dOSqlt_TvkonWuvsFdT5in_A8JGf6rgj35_X-948EsclVqheOVpT8K9vOty8RzHMdfeHKSlQA5TTHCp0H$ >
   and related job types including Patch CTF Estimation
   <https://urldefense.com/v3/__https://guide.cryosparc.com/processing-data/all-job-types-in-cryosparc/ctf-estimation/job-patch-ctf-estimation__;!!Mih3wA!H-jDVE5dOSqlt_TvkonWuvsFdT5in_A8JGf6rgj35_X-948EsclVqheOVpT8K9vOty8RzHMdfeHKSlQA5XH8evsI$ >
   .
   - Cryo-EM Foundations
   <https://urldefense.com/v3/__https://guide.cryosparc.com/cryo-em-foundations/image-formation/contrast-in-cryo-em__;!!Mih3wA!H-jDVE5dOSqlt_TvkonWuvsFdT5in_A8JGf6rgj35_X-948EsclVqheOVpT8K9vOty8RzHMdfeHKSlQA5U5XYizu$ >:
   A new section has been added to the CryoSPARC Guide containing discussions
   around cryo-EM theory useful for understanding the physical and
   computational processes behind imaging and reconstruction.
   - Case Study: End to end processing of an inactive GPCR (EMPIAR-10668).
   We cover end to end processing for a 73 kDa membrane protein
   <https://urldefense.com/v3/__https://guide.cryosparc.com/processing-data/tutorials-and-case-studies/case-study-end-to-end-processing-of-an-inactive-gpcr-empiar-10668__;!!Mih3wA!F7lU4vnfrElwJu81J8p8v6YzBYEcNcmS0WkUZ0eunjKH0awGsb52dKqFjH72vArrFoNmUF33F1_Fogow9rLYfS1A$>
through
   to generating maps ready for model building, as well as analyzing and
   separating states within a continuous motion.

We are continually updating our educational materials and guides. Please
feel free to ask questions about these or any other topic on the CryoSPARC
Discussion Forum <https://urldefense.com/v3/__http://discuss.cryosparc.com__;!!Mih3wA!H-jDVE5dOSqlt_TvkonWuvsFdT5in_A8JGf6rgj35_X-948EsclVqheOVpT8K9vOty8RzHMdfeHKSlQA5b0vP4Bf$ >.

Thank you to all users for your engagement and support.

Happy holidays and happy processing!

The CryoSPARC Team
Structura Biotechnology Inc.
cryosparc.com
structura.bio
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