<div dir="ltr"><div><p>Dear 3DEM,</p><p>Please see below for a roundup of CryoSPARC and cryo-EM data processing educational materials released over the past few months:</p><ul>
<li>Tutorial Videos: Recordings of the <a href="https://urldefense.com/v3/__https://www.youtube.com/playlist?list=PL39mLm0042zJk_ZkM2_OaIoL78uYj6JAb__;!!Mih3wA!H-jDVE5dOSqlt_TvkonWuvsFdT5in_A8JGf6rgj35_X-948EsclVqheOVpT8K9vOty8RzHMdfeHKSlQA5QwjLiTN$">CryoSPARC tutorials</a> from this year's Stanford-SLAC CryoEM Center (S2C2) Image Processing Workshop are available. Over the course of six videos, we delve into cryo-EM fundamentals, a standard workflow for processing SPA datasets, considerations for working with symmetric samples, and ways to deal with discrete and continuous heterogeneity, using four EMPIAR datasets as examples. We are very grateful for S2C2's invitation and encourage users to check out other speakers' talks on <a href="https://urldefense.com/v3/__https://s2c2.slac.stanford.edu/training/workshops-lectures/workshop-lecture-archive__;!!Mih3wA!H-jDVE5dOSqlt_TvkonWuvsFdT5in_A8JGf6rgj35_X-948EsclVqheOVpT8K9vOty8RzHMdfeHKSlQA5RMklgA4$">S2C2's website</a>.</li>
<li>Case Study: <a href="https://urldefense.com/v3/__https://guide.cryosparc.com/processing-data/tutorials-and-case-studies/case-study-end-to-end-processing-of-encapsulated-ferritin-empiar-10716__;!!Mih3wA!H-jDVE5dOSqlt_TvkonWuvsFdT5in_A8JGf6rgj35_X-948EsclVqheOVpT8K9vOty8RzHMdfeHKSlQA5REldEfF$">End to end processing of encapsulin and encapsulated ferritin</a> (EMPIAR-10716), with a focus on challenges and strategies for handling mixed high-order and non-point-group symmetries.</li>
<li>Updated Guide Pages - CTF Estimation: We have released updated guide pages for <a href="https://urldefense.com/v3/__https://guide.cryosparc.com/processing-data/all-job-types-in-cryosparc/ctf-estimation__;!!Mih3wA!H-jDVE5dOSqlt_TvkonWuvsFdT5in_A8JGf6rgj35_X-948EsclVqheOVpT8K9vOty8RzHMdfeHKSlQA5TTHCp0H$">CTF Estimation (theory)</a> and related job types including <a href="https://urldefense.com/v3/__https://guide.cryosparc.com/processing-data/all-job-types-in-cryosparc/ctf-estimation/job-patch-ctf-estimation__;!!Mih3wA!H-jDVE5dOSqlt_TvkonWuvsFdT5in_A8JGf6rgj35_X-948EsclVqheOVpT8K9vOty8RzHMdfeHKSlQA5XH8evsI$">Patch CTF Estimation</a>.</li>
<li><a href="https://urldefense.com/v3/__https://guide.cryosparc.com/cryo-em-foundations/image-formation/contrast-in-cryo-em__;!!Mih3wA!H-jDVE5dOSqlt_TvkonWuvsFdT5in_A8JGf6rgj35_X-948EsclVqheOVpT8K9vOty8RzHMdfeHKSlQA5U5XYizu$">Cryo-EM Foundations</a>: A new section has been added to the CryoSPARC Guide containing discussions around cryo-EM theory useful for understanding the physical and computational processes behind imaging and reconstruction.</li>
<li>Case Study: End to end processing of an inactive GPCR (EMPIAR-10668). We cover <a href="https://urldefense.com/v3/__https://guide.cryosparc.com/processing-data/tutorials-and-case-studies/case-study-end-to-end-processing-of-an-inactive-gpcr-empiar-10668__;!!Mih3wA!F7lU4vnfrElwJu81J8p8v6YzBYEcNcmS0WkUZ0eunjKH0awGsb52dKqFjH72vArrFoNmUF33F1_Fogow9rLYfS1A$">end to end processing for a 73 kDa membrane protein</a> through to generating maps ready for model building, as well as analyzing and separating states within a continuous motion.</li>
</ul><p>We are continually updating our educational materials and guides. Please feel free to ask questions about these or any other topic on the <a href="https://urldefense.com/v3/__http://discuss.cryosparc.com__;!!Mih3wA!H-jDVE5dOSqlt_TvkonWuvsFdT5in_A8JGf6rgj35_X-948EsclVqheOVpT8K9vOty8RzHMdfeHKSlQA5b0vP4Bf$">CryoSPARC Discussion Forum</a>.</p><p>Thank you to all users for your engagement and support.</p><p>Happy holidays and happy processing!</p><div><br></div>The CryoSPARC Team<br>Structura Biotechnology Inc.<br><a href="https://urldefense.com/v3/__http://cryosparc.com__;!!Mih3wA!H-jDVE5dOSqlt_TvkonWuvsFdT5in_A8JGf6rgj35_X-948EsclVqheOVpT8K9vOty8RzHMdfeHKSlQA5Un161hl$">cryosparc.com</a><br>structura.bio</div><div><div dir="ltr" class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr"></div></div></div></div>