[3dem] Seeking advice on Lab6 filament life time

[Cazey, Cornelia]

Hi Mufeeda, 

We have changed the Lab6 filament on our Talos L120c for the first time with 4350h on the clock (on ECO, 5µA only). It was barely hanging on by a thread. The current Lab6 filament has already 2400h of use as well, so at 830h, I would highly doubt this is normal (unless it's a faulty filament). What current do you run yours on? 5µA? or higher? 

As far as I am aware, Lab6 lifetime is certified for at least 1000-1500h, so I would contact service and ask them to have a look at the gun, if this is not something you do yourself. On a Tungsten filament I had to adjust gun alignments and heat values several times throughout the lifetime of a filament. Lab6 is more stable, but can still shift a bit. 

Good luck, 

Cornelia 

Cornelia Cazey (She/Her) 
Cryo-EM Facility 
Centre for Structural Systems Biology (CSSB) 
c/o DESY, Building 15 
Notkestr. 85 
22607 Hamburg 
Germany 

phone: +49-(0)40-8998-87706 
email: cornelia.cazey at cssb-hamburg.de 

"If you don't know it's impossible, it's easier to do" -Neil Gaiman 

Sometimes I may send emails outside of core working hours. I do not expect you to read, or respond outside of your own working hours! 

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Today's Topics: 

1. Seeking advice on Lab6 filament life time (Mufeeda C M) 


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Message: 1 
Date: Wed, 11 Oct 2023 14:19:14 +0800 
From: Mufeeda C M <mufeeda.01 at gmail.com> 
To: 3dem at ncmir.ucsd.edu 
Subject: [3dem] Seeking advice on Lab6 filament life time 
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<CAKb=L7Smn+E+fDO_vzR0jaVjJyo9J1-_Wt0StgOh8mURXQDgjQ at mail.gmail.com> 
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Dear 3D EM members, 

We have Talos L120C, recently we noticed that the beam is quite unstable 
(it kind of started flickering). Filament readout shows 830+ hrs. Should we 
replace the filament sooner? 

Is there any disadvantage if we set the filament heating upto 50? 

[image: image.png] 

-- 

Thanks & Regards 
Mufeeda Madathummal 
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