<html><body><div style="font-family: arial, helvetica, sans-serif; font-size: 12pt; color: #000000"><div data-marker="__QUOTED_TEXT__">Hi Mufeeda,<br><br>We have changed the Lab6 filament on our Talos L120c for the first time with 4350h on the clock (on ECO, 5µA only). It was barely hanging on by a thread. The current Lab6 filament has already 2400h of use as well, so at 830h, I would highly doubt this is normal (unless it's a faulty filament). What current do you run yours on? 5µA? or higher?<br><br>As far as I am aware, Lab6 lifetime is certified for at least 1000-1500h, so I would contact service and ask them to have a look at the gun, if this is not something you do yourself. On a Tungsten filament I had to adjust gun alignments and heat values several times throughout the lifetime of a filament. Lab6 is more stable, but can still shift a bit.<br><br>Good luck,<br><br>Cornelia</div><div data-marker="__QUOTED_TEXT__"><br><span style="font-size: 10pt;" data-mce-style="font-size: 10pt;">Cornelia Cazey (She/Her)</span><br><span style="font-size: 10pt;" data-mce-style="font-size: 10pt;">Cryo-EM Facility</span><br><span style="font-size: 10pt;" data-mce-style="font-size: 10pt;">Centre for Structural Systems Biology (CSSB)</span><br><span style="font-size: 10pt;" data-mce-style="font-size: 10pt;">c/o DESY, Building 15</span><br><span style="font-size: 10pt;" data-mce-style="font-size: 10pt;">Notkestr. 85</span><br><span style="font-size: 10pt;" data-mce-style="font-size: 10pt;">22607 Hamburg</span><br><span style="font-size: 10pt;" data-mce-style="font-size: 10pt;">Germany</span><br><br><span style="font-size: 10pt;" data-mce-style="font-size: 10pt;">phone: +49-(0)40-8998-87706</span><br><span style="font-size: 10pt;" data-mce-style="font-size: 10pt;">email: cornelia.cazey@cssb-hamburg.de</span><br><br><span style="font-size: 10pt;" data-mce-style="font-size: 10pt;">"If you don't know it's impossible, it's easier to do" -Neil Gaiman</span><br><br><span style="font-size: 10pt;" data-mce-style="font-size: 10pt;">Sometimes I may send emails outside of core working hours. I do not expect you to read, or respond outside of your own working hours!</span><br><br>----- Original Message -----<br>From: 3dem-request@ncmir.ucsd.edu<br>To: "3dem" <3dem@ncmir.ucsd.edu><br>Sent: Wednesday, 11 October, 2023 08:19:35<br>Subject: 3dem Digest, Vol 194, Issue 12<br><br>Send 3dem mailing list submissions to<br> 3dem@ncmir.ucsd.edu<br><br>To subscribe or unsubscribe via the World Wide Web, visit<br> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem<br>or, via email, send a message with subject or body 'help' to<br> 3dem-request@ncmir.ucsd.edu<br><br>You can reach the person managing the list at<br> 3dem-owner@ncmir.ucsd.edu<br><br>When replying, please edit your Subject line so it is more specific<br>than "Re: Contents of 3dem digest..."<br><br><br>Today's Topics:<br><br> 1. Seeking advice on Lab6 filament life time (Mufeeda C M)<br><br><br>----------------------------------------------------------------------<br><br>Message: 1<br>Date: Wed, 11 Oct 2023 14:19:14 +0800<br>From: Mufeeda C M <mufeeda.01@gmail.com><br>To: 3dem@ncmir.ucsd.edu<br>Subject: [3dem] Seeking advice on Lab6 filament life time<br>Message-ID:<br> <CAKb=L7Smn+E+fDO_vzR0jaVjJyo9J1-_Wt0StgOh8mURXQDgjQ@mail.gmail.com><br>Content-Type: text/plain; charset="utf-8"<br><br>Dear 3D EM members,<br><br>We have Talos L120C, recently we noticed that the beam is quite unstable<br>(it kind of started flickering). Filament readout shows 830+ hrs. Should we<br>replace the filament sooner?<br><br>Is there any disadvantage if we set the filament heating upto 50?<br><br>[image: image.png]<br><br>--<br><br>Thanks & Regards<br>Mufeeda Madathummal<br>-------------- next part --------------<br>An HTML attachment was scrubbed...<br>URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20231011/9f540fca/attachment.html><br>-------------- next part --------------<br>A non-text attachment was scrubbed...<br>Name: image.png<br>Type: image/png<br>Size: 1122507 bytes<br>Desc: not available<br>URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20231011/9f540fca/attachment.png><br><br>------------------------------<br><br>Subject: Digest Footer<br><br>_______________________________________________<br>3dem mailing list<br>3dem@ncmir.ucsd.edu<br>https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem<br><br><br>------------------------------<br><br>End of 3dem Digest, Vol 194, Issue 12<br>*************************************<br></div></div></body></html>