[3dem] Calling all groups who use cryogen mix from Linde-Gas (Germany, maybe Austria, Switzerland?) for plunging

[Dario]

Just for everyones information:
After contacting basically each gas supplier in Germany, I finally found
one that can produce and deliver the liquid Ethane:propane mixture (at
least in Germany)

Nippon-gases
https://urldefense.com/v3/__https://nippongases.com/__;!!Mih3wA!EWcu8XZh3yPCwu6EsK7-hEtzSFtvLJcLuVmsBcMPfQi6BJeV1_kJpKhkAHqLrcZJSuWkePAPJ4sLNBH6RLdij0fTGA$ 

Cornelia Cazey <cornelia.cazey at cssb-hamburg.de> schrieb am Di., 25. Apr.
2023, 13:50:

> Hi Oliver,
>
> Thanks a lot for the info. But why do they only supply in 50l cylinders?
> Or rather why order a 50l cylinder? Economic reasons?
>
> Thanks!
>
> Cornelia
> On 25 Apr 2023, at 13:40, Oliver Hofnagel <
> oliver.hofnagel at mpi-dortmund.mpg.de> wrote:
>>
>> Dear Cornelia,
>>
>>
>> here our supplier for  37% ethane / 63% propane 50l cylinder cryogen mix
>> in Germany:
>>
>>
>>
>>
>>
>>
>>
>> *AIR LIQUIDE Deutschland GmbH Kunden-Service-Center Spezialgase
>> Bataverstraße 47 47809 Krefeld Telefon: (02151) 954-254 **Email :
>> spezialgase.ald at airliquide.com <spezialgase.ald at airliquide.com>*
>>
>>
>>
>> Its important to order a 50l cylinder, not a 10l cylinder !
>>
>> Greetings,
>>
>> Oliver
>>
>>
>> Dr. Oliver Hofnagel
>> Max-Planck-Institute of Molecular Physiology
>> Electron Microscopy
>> Otto-Hahn-Str. 11
>> 44227 Dortmund
>> Germany
>>
>>
>>
>> ------------------------------
>> *Von:* 3dem <3dem-bounces at ncmir.ucsd.edu> im Auftrag von Michael Elbaum <
>> michael.elbaum at weizmann.ac.il>
>> *Gesendet:* Donnerstag, 20. April 2023 16:29:00
>> *An:* r.i.koning at lumc.nl; cornelia.cazey at cssb-hamburg.de;
>> 3dem at ncmir.ucsd.edu
>> *Betreff:* Re: [3dem] Calling all groups who use cryogen mix from
>> Linde-Gas (Germany, maybe Austria, Switzerland?) for plunging
>>
>>
>> Hi.  would it be possible to use two pure tanks and a mixing regulator? I
>> think such regulators are used for welding.
>>
>> Michael
>> ------------------------------
>> *From:* 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of
>> r.i.koning at lumc.nl <r.i.koning at lumc.nl>
>> *Sent:* Thursday, April 20, 2023 5:15:10 PM
>> *To:* cornelia.cazey at cssb-hamburg.de; 3dem at ncmir.ucsd.edu
>> *Subject:* Re: [3dem] Calling all groups who use cryogen mix from
>> Linde-Gas (Germany, maybe Austria, Switzerland?) for plunging
>>
>>
>> Dear Cornelia,
>>
>>
>>
>> Our most recent order of ethane/propane also did not come through from
>> Line because of the same reason. We will have to do with ethane only for
>> now but I prefer the mixture for obvious reasons, though we do manage with
>> ethane only. We did not look for nor found another vendor.
>>
>>
>>
>> Best regards,
>>
>>
>>
>> Roman
>>
>>
>>
>> *Dr. R.I.Koning*
>>
>>
>>
>> Leiden University Medical Center, Cell and Chemical Biology, Electron
>> Microscopy
>>
>> Postal Zone S1-P, P.O.Box 9600, 2300 RC, Leiden, The Netherlands
>>
>>
>>
>> *visiting address.* Einthovenweg 20, Building 2, zone S1-P, room R-90-21
>>
>> *tel.* (31) 71 526 9296 *fax.* (+31) 71 526 8270 *mail.*
>> r.i.koning at lumc.nl *web. *Electron Microscopy Facility
>> <https://urldefense.com/v3/__https://ccb.lumc.nl/electron-microscopy-198__;!!Mih3wA!GxgfkasxXThNdN_k1a9iqn0d9uls-PLaHnrrJAIhFXNITn6ehlzCx_U6V-rQFPvgm8pSGK1CJ1Vd55FxuUK3uvc$>
>>
>>
>>
>> *From:* 3dem <3dem-bounces at ncmir.ucsd.edu> *On Behalf Of *Cazey, Cornelia
>> *Sent:* donderdag 20 april 2023 15:52
>> *To:* 3dem at ncmir.ucsd.edu
>> *Subject:* [3dem] Calling all groups who use cryogen mix from Linde-Gas
>> (Germany, maybe Austria, Switzerland?) for plunging
>>
>>
>>
>> Dear list participants,
>>
>>
>>
>> Our lab is in Germany, and this is a very particular question relating to
>> a German supplier, so apologies for addressing the entire list, but it
>> seemed the easiest way to find groups who may be able to help.
>>
>>
>>
>> We recently learned that Linde-Gas, our supplier for the cryogen mix we
>> use in our facility (37% ethane, rest propane) is no longer able to supply
>> this mixture due to the overall difficulties in the production, age and
>> poor state of their necessary equipment.
>>
>>
>>
>> I spoke with the supplier yesterday and while they would like to continue
>> to offer the mix, they feel there is not enough market to make it feasible.
>> I am now looking for info from groups who are using a cryogen mix rather
>> than pure ethane for plunging (I am not sure, but relatively convinced
>> that the process would be the same for any kind of mixture, so I guess the
>> exact percentages won't matter too much) and have previously sourced
>> this from Linde-Gas, and might be or have already been affected by this
>> decision. If you have been affected, did you find a solution, a different
>> supplier or any other alternative you are willing to share? Or if you
>> didn't yet know, how much would this news affect your ability to work, and
>> would you be interested to try and convince Linde-Gas to keep going? I
>> understand the production line has already been shut down, but could be
>> revived with enough interest, however, it may also come with a price
>> increase (what doesn't).
>>
>>
>>
>> Any help would be greatly appreciated, whether info on alternatives or
>> willingness to push for the supply. I am happy to collect replies to send
>> to the Linde-Gas representative in bulk or, of course, for those who prefer
>> to contact him directly, do so themselves and increase the pressure to keep
>> going/restart production.
>>
>>
>>
>> Thanks a lot for your help!
>>
>>
>>
>> Best wishes,
>>
>>
>>
>> Cornelia Cazey
>>
>>
>>
>> Cornelia Cazey (She/Her)
>>
>> Cryo-EM Facility
>>
>> Centre for Structural Systems Biology (CSSB)
>> c/o DESY, Building 15
>> Notkestr. 85
>> 22607 Hamburg
>> Germany
>>
>>
>> phone: +49-(0)40-8998-87706
>> email:*cornelia.cazey at cssb-hamburg.de <cornelia.cazey at cssb-hamburg.de>*
>>
>> *"If you don't know it's impossible, it's easier to do"* -Neil Gaiman
>>
>>
>>
>> Sometimes I may send emails outside of core working hours. I do not
>> expect you to read, or respond outside of your own working hours!
>>
> _______________________________________________
> 3dem mailing list
> 3dem at ncmir.ucsd.edu
> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
>
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20230601/d0f203b8/attachment.html>