[3dem] Way to read raw pixel size from .eer?

Takanori Nakane tnakane.protein at osaka-u.ac.jp
Fri Jul 21 05:54:52 PDT 2023


Hi,

EER files contain some metadata in TFS-specific
extended TIFF tags (e.g. IFD 65001).
You have to write a script to read it but a dirty way is to just run

strings XXX.eer | less

and go to the bottom.

There is a "sensorPixelSize.height" field.
But I guess this is a physical pixel size of the detector
independent of the magnification.
I don't know if EER contains the pixel size at the sample.

Best regards,

Takanori Nakane

On 2023/07/21 21:46, Ioannis Skalidis wrote:
> Dear all,
> 
> I was looking for the raw pixel size of some acquired data and realized 
> that when I use the 'header' command from the 3dmod package with the 
> -eer modifier to read the header of an .eer file, it always returns a 
> pixel spacing of 1 Å. Of course, I can read the header of the 
> corresponding .mrc files that are generated, but I was thinking if there 
> is a way in unix/macos environments to read the .eer metadata directly. 
> Does anybody have a way to do this?
> 
> Thanks,
> 
> Ioannis.
> 


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