[3dem] [External] Advice for grid storage for a new lab

[Jaime Llodra]

Dear All,

This is a controversial topic.
I am positive some researchers told me pucks are expensive and difficult to
work with. They have managed to use very organized string/falcon systems
where there are no tangles and samples are easy to find and recover.

Good luck,
Jaime Llodrá


On Wed, Jul 5, 2023 at 4:39 PM Morgan, David Gene <dagmorga at indiana.edu>
wrote:

> Abris,
>
> We are in the process of converting from "Falcon tubes on strings" to one
> of the puck systems.  There are definitely higher costs associated with the
> pucks, but our experience so far suggests that they are worth it.  We can
> store more grids more easily and never worry about losing a tube because a
> string breaks or gets cut.  You will need to think about "bookkeeping" but
> is true for any storage system.
>
> I had initially wondered about ice contamination from taking a rack of
> pucks out of the storage dewar every time I dealt with any of the grids,
> but at least so far, I don't think I see that happening.  Others who have
> used similar systems longer may be able to comment on this.
>
> --
>     politics is more difficult than physics.
>                                              A. Einstein
>
>             David Gene Morgan
>         Electron Microscopy Center
>              047E Simon Hall
>              IU Bloomington
>           812 856 1457 (office)
>           812 856 3221 (3200)
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> ------------------------------
> *From:* 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Abris Bendes <
> Abris.Bendes at abo.fi>
> *Sent:* Wednesday, July 5, 2023 6:30 AM
> *To:* 3dem at ncmir.ucsd.edu <3dem at ncmir.ucsd.edu>
> *Subject:* [External] [3dem] Advice for grid storage for a new lab
>
>
> This message was sent from a non-IU address. Please exercise caution when
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>
> Dear all,
>
>
> We are setting up a cryoEM sample preparation lab (mostly for SPA) from
> scratch, and still missing some smaller equipment and consumables. I would
> like to ask for your input on what is regarded as best practices for a
> smaller starting-out lab. What is currently the best option for boxes and
> storage of non-clipped grids? I have seen the emergence of twist-top grid
> boxes (eg from SWISSCI) and pucks (eg MiTeGen Gen 2), but have no
> experience with them. Any suggestions or other tips, not just for storage
> options, for a beginner lab would be most welcome.
>
>
> Best regards,
>
> Abris
>
>
> _______________________________________________
>
> *Ábris Á. Bendes, PhD*
>
> InFLAMES Flagship postdoctoral researcher
>
> Salminen lab / SBL / Åbo Akademi University
>
> Jalkanen and Hollmén lab / MediCity / University of Turku
>
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