<div dir="ltr"><div>Dear All,<br></div><div><br></div><div>This is a controversial topic.</div><div>I am positive some researchers told me pucks are expensive and difficult to work with. They have managed to use very organized string/falcon systems where there are no tangles and samples are easy to find and recover.</div><div><br></div><div>Good luck,</div><div>Jaime Llodrá<br></div><div><br></div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Wed, Jul 5, 2023 at 4:39 PM Morgan, David Gene <<a href="mailto:dagmorga@indiana.edu" target="_blank">dagmorga@indiana.edu</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div>
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Abris,</div>
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We are in the process of converting from "Falcon tubes on strings" to one of the puck systems. There are definitely higher costs associated with the pucks, but our experience so far suggests that they are worth it. We can store more grids more easily and
never worry about losing a tube because a string breaks or gets cut. You will need to think about "bookkeeping" but is true for any storage system.<br>
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I had initially wondered about ice contamination from taking a rack of pucks out of the storage dewar every time I dealt with any of the grids, but at least so far, I don't think I see that happening. Others who have used similar systems longer may be able
to comment on this.<br>
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<div id="m_-7151324196508978776m_-4515881342662359181divRplyFwdMsg" dir="ltr"><font style="font-size:11pt" face="Calibri, sans-serif" color="#000000"><b>From:</b> 3dem <<a href="mailto:3dem-bounces@ncmir.ucsd.edu" target="_blank">3dem-bounces@ncmir.ucsd.edu</a>> on behalf of Abris Bendes <<a href="mailto:Abris.Bendes@abo.fi" target="_blank">Abris.Bendes@abo.fi</a>><br>
<b>Sent:</b> Wednesday, July 5, 2023 6:30 AM<br>
<b>To:</b> <a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a> <<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a>><br>
<b>Subject:</b> [External] [3dem] Advice for grid storage for a new lab</font>
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<p>Dear all,</p>
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<p>We are setting up a cryoEM sample preparation lab (mostly for SPA) from scratch, and still missing some smaller equipment and consumables. I would like to ask for your input on what is regarded as best practices for a smaller starting-out lab. What is currently
the best option for boxes and storage of <span style="font-family:Calibri,Helvetica,sans-serif,EmojiFont,"Apple Color Emoji","Segoe UI Emoji",NotoColorEmoji,"Segoe UI Symbol","Android Emoji",EmojiSymbols;font-size:16px">
non-clipped grids</span>? I have seen the emergence of twist-top grid boxes (eg from SWISSCI) and pucks (eg MiTeGen Gen 2), but have no experience with them. Any suggestions or other tips, not just for storage options, for a beginner lab would be most welcome.</p>
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<p>Best regards,</p>
<p>Abris</p>
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<p>_______________________________________________</p>
<p><b><span style="font-size:11pt">Ábris Á. Bendes, PhD</span></b></p>
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<p><span style="font-size:11pt"></span><span style="font-size:11pt">InFLAMES Flagship postdoctoral researcher</span></p>
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<p><span style="font-size:11pt"></span><span style="font-size:11pt">Salminen lab / SBL / </span><span style="font-size:11pt">Åbo Akademi University</span></p>
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<p><span style="font-size:11pt"></span><span style="font-size:11pt">Jalkanen and Hollmén lab / MediCity / University of Turku</span></p>
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