[3dem] glow discharge question

[Daniel Asarnow]

Hi David,
I believe we used to do this at UCSF simply by putting a small watch glass
with a small amount of the compound inside the bell jar. (The 2nd, older
discharger therefore has the message "DO NOT PUT ANY CHEMICALS INSIDE" on
the device).

A literature search for easiglow and amylamine led to a number of papers
that all refer to "discharging in the presence of amylamine" or similar
wording (e.g. 1
<https://urldefense.com/v3/__https://www.biorxiv.org/content/10.1101/2020.02.01.930206v1.full__;!!Mih3wA!Bk2raXReM_X1BzruHEI3SN8LOGhTBsx_gOcXSeVGy-2tL63jpjzRZYcgnLaQf1EcAuVwk9ZO29odx_9nAbdVGcI$ >, 2
<https://urldefense.com/v3/__https://www.biorxiv.org/content/10.1101/2021.12.09.471496v1.full__;!!Mih3wA!Bk2raXReM_X1BzruHEI3SN8LOGhTBsx_gOcXSeVGy-2tL63jpjzRZYcgnLaQf1EcAuVwk9ZO29odx_9nU6HtRQw$ >, 3
<https://urldefense.com/v3/__https://www.nature.com/articles/s41594-020-0454-0__;!!Mih3wA!Bk2raXReM_X1BzruHEI3SN8LOGhTBsx_gOcXSeVGy-2tL63jpjzRZYcgnLaQf1EcAuVwk9ZO29odx_9nqBUN894$ >); I bet they are also
just introducing an aliquot of compound into the chamber, rather than
creating a sealed tube connected to the pump as in this old work of Dubochet
<https://urldefense.com/v3/__https://www.sciencedirect.com/science/article/pii/S002253207180148X__;!!Mih3wA!Bk2raXReM_X1BzruHEI3SN8LOGhTBsx_gOcXSeVGy-2tL63jpjzRZYcgnLaQf1EcAuVwk9ZO29odx_9nm5vObrs$ >.

Ted Pella's product page gives Hayat, M.A.2000. Principles and Techniques
of Electron Microscopy Biological Applications, page 237-241 (which I do
not have access to) as a reference for using amylamine.

Best,
-da

On Wed, Jan 11, 2023 at 1:43 PM Morgan, David Gene <dagmorga at indiana.edu>
wrote:

> Hi, Has anyone modified a Pelco glow discharge device so that one can
> introduce something like amylamine into the chamber during the glow
> discharge? I can think of several ways that this could be done, but if
> someone already has a working model,
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> Hi,
>
> Has anyone modified a Pelco glow discharge device so that one can
> introduce something like amylamine into the chamber during the glow
> discharge?  I can think of several ways that this could be done, but if
> someone already has a working model, I would love to chat with them.
>
> Thanks in advance.
>
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