<div dir="ltr"><div>Hi David,</div><div>I believe we used to do this at UCSF simply by
putting a small watch glass with a small amount of the compound inside
the bell jar. (The 2nd, older discharger therefore has the message "DO <strike>NOT</strike> PUT ANY CHEMICALS INSIDE" on the device).</div><div><br></div><div>A literature search for easiglow and amylamine led to a number of papers that all refer to "discharging in the presence of amylamine" or similar wording (e.g. <a href="https://urldefense.com/v3/__https://www.biorxiv.org/content/10.1101/2020.02.01.930206v1.full__;!!Mih3wA!Bk2raXReM_X1BzruHEI3SN8LOGhTBsx_gOcXSeVGy-2tL63jpjzRZYcgnLaQf1EcAuVwk9ZO29odx_9nAbdVGcI$">1</a>, <a href="https://urldefense.com/v3/__https://www.biorxiv.org/content/10.1101/2021.12.09.471496v1.full__;!!Mih3wA!Bk2raXReM_X1BzruHEI3SN8LOGhTBsx_gOcXSeVGy-2tL63jpjzRZYcgnLaQf1EcAuVwk9ZO29odx_9nU6HtRQw$">2</a>, <a href="https://urldefense.com/v3/__https://www.nature.com/articles/s41594-020-0454-0__;!!Mih3wA!Bk2raXReM_X1BzruHEI3SN8LOGhTBsx_gOcXSeVGy-2tL63jpjzRZYcgnLaQf1EcAuVwk9ZO29odx_9nqBUN894$">3</a>); I bet they are also just introducing an aliquot of compound into the chamber, rather than creating a sealed tube connected to the pump as in <a href="https://urldefense.com/v3/__https://www.sciencedirect.com/science/article/pii/S002253207180148X__;!!Mih3wA!Bk2raXReM_X1BzruHEI3SN8LOGhTBsx_gOcXSeVGy-2tL63jpjzRZYcgnLaQf1EcAuVwk9ZO29odx_9nm5vObrs$">this old work of Dubochet</a>.<br></div><div><br></div><div>Ted Pella's product page gives Hayat, M.A.2000. Principles and Techniques of Electron Microscopy Biological Applications, page 237-241 (which I do not have access to) as a reference for using amylamine.<br></div><div><br></div><div>Best,</div><div>-da</div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Wed, Jan 11, 2023 at 1:43 PM Morgan, David Gene <<a href="mailto:dagmorga@indiana.edu">dagmorga@indiana.edu</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div class="msg-4684459185140755207">
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Hi, Has anyone modified a Pelco glow discharge device so that one can introduce something like amylamine into the chamber during the glow discharge? I can think of several ways that this could be done, but if someone already has a working model,
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<div><span style="font-family:Calibri,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0);background-color:rgb(255,255,255)">Hi,</span></div>
<div><span style="font-family:Calibri,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0);background-color:rgb(255,255,255)"><br>
</span></div>
<div><span style="font-family:Calibri,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0);background-color:rgb(255,255,255)">Has anyone modified a Pelco glow discharge device so that one can introduce something like amylamine
into the chamber during the glow discharge? I can think of several ways that this could be done, but if someone already has a working model, I would love to chat with them.</span></div>
<div><span style="font-family:Calibri,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0);background-color:rgb(255,255,255)"><br>
</span></div>
<div><span style="font-family:Calibri,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0);background-color:rgb(255,255,255)">Thanks in advance.<br>
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