[3dem] Cryo-EM facilities operating in high biosafety containment

[Chipman,Paul]

We had a basic (FEI CM120) cryo-EM instrument in BSL3 space in Winnipeg at the Canadian Centre for Foreign Animal Disease .  Access to BSL4 as well.  I’ve not been there in 20 years so cannot comment on current capabilities..

At Purdue we had a CM200 installed in BSL3 space but also have not been there in over a decade and cannot comment of current capabilities.

I send any requests I get for such functionality to Misha Sherman in Galveston.    Great guy.  Great work. Great facility.

Best,

[Text, logo  Description automatically generated]
Paul Chipman
Scientific Director
352.294.1790 | ICBR-ElectronMicroscopy at ad.ufl.edu<mailto:ICBR-ElectronMicroscopy at ad.ufl.edu> | biotech.ufl.edu<https://urldefense.com/v3/__https://biotech.ufl.edu/__;!!Mih3wA!B0XxK6c_elZMFT9BdnYbzYi-_oTcQb8O-qPFdjJrlkVVCL1K92Qs9vtyYSbW0Jh-ZVCjFrWeFFqSQPfBR11C$ >
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Paul

From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Sherman, Michael B. <mbsherma at UTMB.EDU>
Date: Friday, April 14, 2023 at 12:49 PM
To: Petr Chlanda <pchlanda at gmail.com>, 3dem at ncmir.ucsd.edu <3dem at ncmir.ucsd.edu>
Subject: Re: [3dem] Cryo-EM facilities operating in high biosafety containment
[External Email]
Dear Petr,
Yes, we are doing BSL3 cryo-EM in the lab. We have a BSL3 containment in which JEOL 2200FS microscope is installed. The lab is operational for many years now, and yes, you could include us in the list.
All the best,
Misha

Michael Sherman, PhD
Dept. of Biochem. & Mol. Biology
1.224 Medical Research Building
Univ. of Texas Medical Branch
Galveston, TX 77555-1055
Tel. 409-772-6310
Fax: 409-772-6299
mbsherma at UTMB.edu<mailto:mbsherma at UTMB.edu>


From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Petr Chlanda <pchlanda at gmail.com>
Date: Friday, April 14, 2023 at 11:40 AM
To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
Subject: [3dem] Cryo-EM facilities operating in high biosafety containment


External Email Warning: Do not click links or open attachments unless you recognize the sender and expect the content. UTMB Email Phishing Awareness<https://urldefense.com/v3/__https:/www.utmb.edu/infosec/resources/phishawareness__;!!Mih3wA!FrPu4IMKreWrvhNmpIkHRe8pv65jUD5v-NH2nu50mRGuWA5SHiKaIekXDVR5m6i0T0pjiI3aC7e6Nvw1IKj0lg$>
Dear all,

We are writing a short review on cryo-ET approaches to study virus-host interactions where we also discuss pathogen inactivation protocols for cryo-EM and access to cryo-EM operating at high biosafety containment. There are certainly many sites where cryo-EM can be done at BSL2 but it is not so obvious how many and which academic institutions around the world can perform cryo-EM on BSL3 pathogens. As this information might be useful to the cryo-EM community focusing on infectious diseases we would like to include it. Could you please let me know if you are able to perform cryo-EM at certified BSL3 (or even BSL4) and if we can list your facility in the review?

Perhaps information on BSL could also be included in future on the Google map of High-End Cryo-EM Worldwide.
https://urldefense.com/v3/__https://www.google.com/maps/d/viewer?mid=1eQ1r8BiDYfaK7D1S9EeFJEgkLggMyoaT&hl=en_US&ll=46.94318447040963*2C16.6782303119493&z=4__;JQ!!Mih3wA!B0XxK6c_elZMFT9BdnYbzYi-_oTcQb8O-qPFdjJrlkVVCL1K92Qs9vtyYSbW0Jh-ZVCjFrWeFFqSQGWLuAPH$ <https://urldefense.com/v3/__https:/www.google.com/maps/d/viewer?mid=1eQ1r8BiDYfaK7D1S9EeFJEgkLggMyoaT&hl=en_US&ll=46.94318447040963*2C16.6782303119493&z=4__;JQ!!Mih3wA!Ayx3Z0fmKqEIseneNlQp1-d0amDUwwetSr1e32v3z0USQQh1j-pR90apBYSBQ63yTc9V28exKiI6e7VaTBA$>

Thank you in advance!

Best wishes,
Petr


Petr Chlanda
Schaller Group Leader
Membrane Biology of Viral Infection<https://urldefense.com/v3/__http:/www.bioquant.uni-heidelberg.de/research/junior-research-groups/chs-research-group-membrane-biology-of-viral-infection.html__;!!Mih3wA!Ayx3Z0fmKqEIseneNlQp1-d0amDUwwetSr1e32v3z0USQQh1j-pR90apBYSBQ63yTc9V28exKiI67nAPZKo$>
Department of Infectious Diseases -Virology
University Hospital Heidelberg

BioQuant BQ-0050
Im Neuenheimer Feld 267, Rm 102a
D-69120 Heidelberg

Tel: +49 (0)6221 54-51231
Fax:+49 (0)6221 54-51480
petr.chlanda at bioquant.uni-heidelberg.de<mailto:petr.chlanda at bioquant.uni-heidelberg.de>

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