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<p class="MsoNormal"><span style="font-size:11.0pt">We had a basic (FEI CM120) cryo-EM instrument in BSL3 space in Winnipeg at the Canadian Centre for Foreign Animal Disease . Access to BSL4 as well. I’ve not been there in 20 years so cannot comment on current
capabilities..<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">At Purdue we had a CM200 installed in BSL3 space but also have not been there in over a decade and cannot comment of current capabilities.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">I send any requests I get for such functionality to Misha Sherman in Galveston. Great guy. Great work. Great facility.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Best,<o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:14.0pt;color:#00519C">Paul Chipman</span></b><b><span style="color:#00519C"><o:p></o:p></span></b></p>
<p class="MsoNormal"><i><span style="font-size:11.0pt">Scientific Director<o:p></o:p></span></i></p>
<p class="MsoNormal"><span style="font-size:9.0pt;letter-spacing:1.0pt">352.294.1790
<b>|</b> </span><span style="font-size:11.0pt"><a href="mailto:ICBR-ElectronMicroscopy@ad.ufl.edu"><span style="font-size:9.0pt;color:#0563C1;letter-spacing:1.0pt">ICBR-ElectronMicroscopy@ad.ufl.edu</span></a></span><span style="font-size:9.0pt;letter-spacing:1.0pt">
<b>|</b> </span><span style="font-size:11.0pt"><a href="https://urldefense.com/v3/__https://biotech.ufl.edu/__;!!Mih3wA!B0XxK6c_elZMFT9BdnYbzYi-_oTcQb8O-qPFdjJrlkVVCL1K92Qs9vtyYSbW0Jh-ZVCjFrWeFFqSQPfBR11C$"><span style="font-size:9.0pt;color:#0563C1;letter-spacing:1.0pt">biotech.ufl.edu</span></a></span><u><span style="font-size:9.0pt;color:#0563C1;letter-spacing:1.0pt"><o:p></o:p></span></u></p>
<p class="MsoNormal"><b><span style="font-size:9.0pt;letter-spacing:1.0pt">RRID:SCR_019146
</span></b><span style="font-size:11.0pt"><a href="https://urldefense.com/v3/__https://biotech.ufl.edu/acknowledging-icbr/__;!!Mih3wA!B0XxK6c_elZMFT9BdnYbzYi-_oTcQb8O-qPFdjJrlkVVCL1K92Qs9vtyYSbW0Jh-ZVCjFrWeFFqSQBNQRJTi$"><b><i><span style="font-size:9.0pt;color:#0563C1;letter-spacing:1.0pt">Acknowledge ICBR</span></i></b></a><b><i><span style="color:#002060"><o:p></o:p></span></i></b></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Paul<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
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<p class="MsoNormal" style="margin-bottom:12.0pt"><b><span style="font-size:12.0pt;color:black">From:
</span></b><span style="font-size:12.0pt;color:black">3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Sherman, Michael B. <mbsherma@UTMB.EDU><br>
<b>Date: </b>Friday, April 14, 2023 at 12:49 PM<br>
<b>To: </b>Petr Chlanda <pchlanda@gmail.com>, 3dem@ncmir.ucsd.edu <3dem@ncmir.ucsd.edu><br>
<b>Subject: </b>Re: [3dem] Cryo-EM facilities operating in high biosafety containment<o:p></o:p></span></p>
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<b><span style="font-size:9.0pt;font-family:"Arial",sans-serif;color:#FA4616">[External Email]</span></b><span style="font-size:11.0pt">
<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt">Dear Petr,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Yes, we are doing BSL3 cryo-EM in the lab. We have a BSL3 containment in which JEOL 2200FS microscope is installed. The lab is operational for many years now, and yes, you could include us in the list.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">All the best,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Misha<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"> <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;color:black">Michael Sherman, PhD<br>
Dept. of Biochem. & Mol. Biology<br>
1.224 Medical Research Building<br>
Univ. of Texas Medical Branch<br>
Galveston, TX 77555-1055<br>
Tel. 409-772-6310<br>
Fax: 409-772-6299<br>
</span><span style="font-size:11.0pt"><a href="mailto:mbsherma@UTMB.edu"><span style="color:purple">mbsherma@UTMB.edu</span></a><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt"> <o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"> <o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:12.0pt;color:black">From: </span></b><span style="font-size:12.0pt;color:black">3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Petr Chlanda <pchlanda@gmail.com><br>
<b>Date: </b>Friday, April 14, 2023 at 11:40 AM<br>
<b>To: </b>"3dem@ncmir.ucsd.edu" <3dem@ncmir.ucsd.edu><br>
<b>Subject: </b>[3dem] Cryo-EM facilities operating in high biosafety containment</span><span style="font-size:11.0pt"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt"> <o:p></o:p></span></p>
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<span style="font-size:11.0pt;color:black"> </span><span style="font-size:11.0pt"><o:p></o:p></span></p>
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<b><span style="font-size:12.0pt;color:#111111">External Email Warning:</span></b><span style="font-size:12.0pt;color:#111111"> Do not click links or open attachments unless you recognize the sender and expect the content.
<a href="https://urldefense.com/v3/__https:/www.utmb.edu/infosec/resources/phishawareness__;!!Mih3wA!FrPu4IMKreWrvhNmpIkHRe8pv65jUD5v-NH2nu50mRGuWA5SHiKaIekXDVR5m6i0T0pjiI3aC7e6Nvw1IKj0lg$">
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<p class="MsoNormal"><span style="font-family:"Verdana Pro",sans-serif">Dear all,</span><span style="font-size:11.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Verdana Pro",sans-serif"> </span><span style="font-size:11.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Verdana Pro",sans-serif">We are writing a short review on cryo-ET approaches to study virus-host interactions where we also discuss pathogen inactivation protocols for cryo-EM and access to cryo-EM operating at
high biosafety containment. There are certainly many sites where cryo-EM can be done at BSL2 but it is not so obvious how many and which academic institutions around the world can perform cryo-EM on BSL3 pathogens. As this information might be useful to the
cryo-EM community focusing on infectious diseases we would like to include it. Could you please let me know if you are able to perform cryo-EM at certified BSL3 (or even BSL4) and if we can list your facility in the review?</span><span style="font-size:11.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Verdana Pro",sans-serif"> </span><span style="font-size:11.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Verdana Pro",sans-serif">Perhaps information on BSL could also be included in future on the Google map of High-End Cryo-EM Worldwide.</span><span style="font-size:11.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Verdana Pro",sans-serif"><a href="https://urldefense.com/v3/__https:/www.google.com/maps/d/viewer?mid=1eQ1r8BiDYfaK7D1S9EeFJEgkLggMyoaT&hl=en_US&ll=46.94318447040963*2C16.6782303119493&z=4__;JQ!!Mih3wA!Ayx3Z0fmKqEIseneNlQp1-d0amDUwwetSr1e32v3z0USQQh1j-pR90apBYSBQ63yTc9V28exKiI6e7VaTBA$"><span style="font-family:"Times New Roman",serif;color:black">https://www.google.com/maps/d/viewer?mid=1eQ1r8BiDYfaK7D1S9EeFJEgkLggMyoaT&hl=en_US&ll=46.94318447040963%2C16.6782303119493&z=4</span></a>
</span><span style="font-size:11.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Verdana Pro",sans-serif"> </span><span style="font-size:11.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Verdana Pro",sans-serif">Thank you in advance!</span><span style="font-size:11.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Verdana Pro",sans-serif"> </span><span style="font-size:11.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Verdana Pro",sans-serif">Best wishes,</span><span style="font-size:11.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Verdana Pro",sans-serif">Petr</span><span style="font-size:11.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Verdana Pro",sans-serif"> </span><span style="font-size:11.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Verdana Pro",sans-serif"> </span><span style="font-size:11.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><i><span style="font-size:8.0pt;font-family:"Arial",sans-serif">Petr Chlanda</span></i><span style="font-size:11.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><i><span style="font-size:8.0pt;font-family:"Arial",sans-serif">Schaller Group Leader<br>
</span></i><span style="font-size:11.0pt"><a href="https://urldefense.com/v3/__http:/www.bioquant.uni-heidelberg.de/research/junior-research-groups/chs-research-group-membrane-biology-of-viral-infection.html__;!!Mih3wA!Ayx3Z0fmKqEIseneNlQp1-d0amDUwwetSr1e32v3z0USQQh1j-pR90apBYSBQ63yTc9V28exKiI67nAPZKo$"><i><span style="font-size:8.0pt;font-family:"Arial",sans-serif;color:black">Membrane
Biology of Viral Infection</span></i></a><o:p></o:p></span></p>
<p class="MsoNormal"><i><span style="font-size:8.0pt;font-family:"Arial",sans-serif;color:black">Department of Infectious Diseases -Virology</span></i><span style="font-size:11.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><i><span style="font-size:8.0pt;font-family:"Arial",sans-serif;color:black">University Hospital Heidelberg</span></i><span style="font-size:11.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><i><span style="font-size:8.0pt;font-family:"Arial",sans-serif"> </span></i><span style="font-size:11.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><i><span lang="DE" style="font-size:8.0pt;font-family:"Arial",sans-serif;color:black">BioQuant BQ-0050</span></i><span style="font-size:11.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><i><span lang="DE" style="font-size:8.0pt;font-family:"Arial",sans-serif;color:black">Im Neuenheimer Feld 267, Rm 102a<br>
D-69120 Heidelberg</span></i><span style="font-size:11.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><i><span lang="DE" style="font-size:8.0pt;font-family:"Arial",sans-serif"> </span></i><span style="font-size:11.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><i><span lang="DE" style="font-size:8.0pt;font-family:"Arial",sans-serif;color:black">Tel: +49 (0)6221 54-51231</span></i><span style="font-size:11.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><i><span lang="DE" style="font-size:8.0pt;font-family:"Arial",sans-serif">Fax:+49 (0)6221 54-51480<br>
<u><span style="color:blue"><a href="mailto:petr.chlanda@bioquant.uni-heidelberg.de"><span style="font-family:"Times New Roman",serif;color:black">petr.chlanda@bioquant.uni-heidelberg.de</span></a></span></u></span></i><span style="font-size:11.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><i><span lang="DE" style="font-size:8.0pt;font-family:"Arial",sans-serif"> </span></i><span style="font-size:11.0pt"><o:p></o:p></span></p>
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