[3dem] [EXTERNAL] Re: 3D printing for Clipping station

Daniel Asarnow asarnow at msg.ucsf.edu
Tue Apr 18 09:34:18 PDT 2023


Hi Esther,
That tray looks cool! Is the .stl file available anywhere?

Best,
-da

On Tue, Apr 18, 2023 at 8:03 AM Bullitt, Esther <bullitt at bu.edu> wrote:

> Hi Bob, A collaborator 3D printed this grid transfer tray (see photo
> attached), from PLA plastic, and it has worked fine in LN2 for the past 9
> years. It is a little less than 3” across. Good luck with it, Esther On Apr
> 17, 2023, at 6: 10 PM,
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> Hi Bob,
>
> A collaborator 3D printed this grid transfer tray (see photo attached),
> from PLA plastic, and it has worked fine in LN2 for the past 9 years.
> It is a little less than 3” across.
>
> Good luck with it,
> Esther
>
> On Apr 17, 2023, at 6:10 PM, Grassucci, Robert <rg2502 at cumc.columbia.edu>
> wrote:
>
> Thanks Farzad,
> Machining seems to be the longest lasting option but as you say the
> fastest solution is just putting the metal part into a styrofoam box.
> Bob
> ------------------------------
> *From:* Farzad <farzaad at gmail.com>
> *Sent:* Monday, April 17, 2023 6:06 PM
> *To:* Grassucci, Robert <rg2502 at cumc.columbia.edu>
> *Cc:* 3DEM List (3dem at ncmir.ucsd.edu) <3dem at ncmir.ucsd.edu>; cryoem <
> cryoem at cumc.columbia.edu>
> *Subject:* [EXTERNAL] Re: [3dem] 3D printing for Clipping station
>
> Dear Robert,
>
> First of all, I am not sure if 3D printing works fine and if the part
> survives for a long time in harsh thermal cycling between LN2 and room
> temperatures, especially with normal filaments injection (FDM) 3D printers.
>
> Anyways, as a materials scientist (formerly an expert in materials
> selection and now working as a cryoEM lab manager), I would definitely
> suggest using polyamide (Nylon) filaments for printing cryogenic parts in
> FDM printers. Normal PLA and ABS filaments won't work.
>
>
> If you like to have the base in its original shape, I would suggest
> ordering it to a machining shop instead of printing it. The recommend
> materials are POM, PA or PC.
>
> On the other hand, why do you want to make it entirely? If the metal parts
> are still fine, you need just a base to hold it. You can fit the metal
> mechanism and the fabricated base in any foam compartment like cheap
> styrofoam boxes of an appropriate size. We use a primitive white foam box
> for loading our JEOL for ages... In this case, you could mill that part out
> of polyacetal (aka POM or polyoxymethylene or under the commercial name of
> Delrin) which is the cheapest option for a polymer at LN2 service. I guess
> the original box is also made of PIM.  I would recommend polyamide, though.
>
>
> Best regards,
>
> Dr. Farzad Hamdi,
> Scientist Fellow at AG Kastritis
> MLU, Halle
>
> On Mon, Apr 17, 2023, 22:39 Grassucci, Robert <rg2502 at cumc.columbia.edu>
> wrote:
>
> Hi All,
> Does anyone have a file for 3D printing the plastic liner for the TFS
> clipping station?  Ours is cracked so I am looking to replace it.  Also
> which material is recommended to use?  Thank you.
> Bob
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