[3dem] Interpretation of strange objects in a cryo-EM sample

[Xinghong Dai]

Not many things can be "lighter" than water in electron density. I would
say they are just air bubbles. You can easily make bubbles by pipetting
when there is detergent.


Best regards,

Xinghong

_____________________________________________________

Xinghong Dai, Ph.D.

Assistant Professor

Department of Physiology and Biophysics

Case Western Reserve University, School of Medicine

Email: xinghong.dai at case.edu

Office: +1 216-368 5131            Cell: +1 216-232 0939

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On Thu, Apr 13, 2023 at 9:48 AM Kizziah, James <kizziah4 at uab.edu> wrote:

> Hi all,
>
>
>
> We have imaged a cryo-EM sample presumed to contain micelles and observed
> the large, white objects full of/covered with junk shown in the attached
> images. Our best guess is that these are (or were) the micelles and have
> been destroyed by the electron beam, contact with the air water interface,
> or both, after vitrification. Has anyone seen something like this before or
> does anyone have alternative interpretations?
>
>
>
> Glow-discharged lacey carbon grids plunge frozen with a Mark IV Vitrobot,
> clipped and imaged on a Glacios.
>
>
>
> Thanks,
>
> James
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