[3dem] Nano Webinar: Advanced Cryo Preparation Techniques | 11th October

Fri Sep 30 01:14:42 PDT 2022

Dear 3DEM community please be aware that there will be another great
webinar lined up, this time on Cryo-Fib and Cryo-EM, check the link below!!

Dr. Andreia Lucia Pinto
Electron Microscopist
Advanced Workflow Application Specialist @ Leica Microsystems
Honorary Research Associate of the Imperial College London
+49 (0) 1743132456    LinkedIn: Andreia Lucia do Nascimento Pinto
Twitter:@cellfie3

EM Sample Preparation Webinar, October 11th

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How to Prepare Biological Samples for cryo-FIB Milling and cryo-EM Tuesday
11th October: 10:00 London | 11:00 Berlin | 13:00 Dubai

Cryo-electron microscopy is becoming an important tool in biomedical
research, allowing researchers to explore the cellular landscape in its
native state and at high spatial resolution. A prerequisite to obtaining
that goal is the preparation of electron transparent samples through
cryo-FIB milling. This webinar highlights all steps in the workflow, from
capturing the live sample to the perfect cryo-EM image.

In this webinar, experts from Human Technopole and Leica Microsystems will
cover the different tools used to prepare cellular and biological samples,
from initial vitrification and high pressure freezing, towards cryo-planing
of bulk samples and confocal microscopy under cryo conditions.

A panel discussion will follow the presentations, during which our experts
will take questions from the audience.

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*Also available on demand - register your interest above.*
Key Learnings:

   - How to effectively vitrify samples for on-grid lamella and lift-out
   procedures
   - Cryo-planing methodology for bulk samples
   - Cryo-CLEM acquisition routines and best practices
   - Illustrations of samples prepared via FIB milling

*Speakers / Panelists: *

*Philipp Erdmann*
*Research Group Leader, Erdmann Group, Human Technopole*
Philipp is a chemical biologist and microscopist. After five years as a
project group leader at the MPI for Biochemistry, Martinsried, he joined HT
in February 2021. Philipp’s lab focuses on the analysis of liquid-liquid
phase separation (LLPS) effects using in situ cryo-electron tomography.
LLPS is often involved in crucial processes of both transmittable and
non-transmittable human diseases, including virus infections and
neurodegenerative disorders.

*Malan Silva*
*Electron Microscopy Specialist, Cryo-EM Facility, Human Technopole*
Malan is a structural neuroscientist specializing in cryo- and
plastic-section-based EM methods. Malan trained in ultramicrotomy and
electron microscopy, and in his postdoctoral studies he developed cryo
sectioning-based sample preparation pipelines to study the ultrastructure
of neurotransmission. Currently, at Human Technopole, Malan is developing
FIB-SEM-based sample preparation methods. Malan plans to unify the cryo and
room-temperature sample preparation methods to maximize the scientific
potential of existing technologies.

*Frédéric Leroux*
*Advanced Workflow Manager, Leica Microsystems*
Frédéric completed his Master's degree in Biology at the University of
Ghent where he gained experience in biological EM sample preparation.
During his PhD at the EMAT research group University of Antwerp, he
specialized in advanced electron microscopy of composite materials, going
on to become EM sample preparation specialist at EMAT. In 2016, he joined
Leica Microsystems as Application Specialist Nanotechnology, where he uses
his multidisciplinary background and broad microscopy experience to improve
EM sample preparation for a variety of materials (polymers, composites,
biological and industrial materials).

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------------------------------

Best regards

Your Leica Microsystems Nanotechnology Team

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Dr. Andreia Lucia Pinto
Electron Microscopist
Advances Workflow Application Specialist @ Leica Microsystems
Honorary Research Associate of the Imperial College London
+49 (0) 1743132456    LinkedIn: Andreia Lucia do Nascimento Pinto
Twitter:@cellfie3
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