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<br><div style="text-align:left">Dear 3DEM community please be aware that there will be another great webinar lined up, this time on Cryo-Fib and Cryo-EM, check the link below!!</div><div style="text-align:left"><br></div><div style="text-align:left"><font face="arial black, sans-serif">Dr. Andreia Lucia Pinto</font><div>Electron Microscopist</div><div>Advanced Workflow Application Specialist @ Leica Microsystems</div><div><font size="1">Honorary Research Associate of the Imperial College London</font></div><div><font size="1">+49 (0) 1743132456 LinkedIn: Andreia Lucia do Nascimento Pinto</font><span style="font-size:x-small"> Twitter:@cellfie3</span></div></div><br><br><table class="gmail-MsoNormalTable" border="0" cellspacing="0" cellpadding="0" width="100%" style="width:100%;background:rgb(238,238,238);border-collapse:collapse">
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<p class="MsoNormal" style="line-height:9.4pt;margin:0in;font-size:11pt;font-family:Calibri,sans-serif"><span style="font-size:7.5pt;font-family:Helvetica,sans-serif;color:gray">EM Sample Preparation Webinar, October 11th</span></p>
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<a href="https://urldefense.com/v3/__https://www2.leica-microsystems.com/webmail/149501/1106960008/ac89d18dd9840be9c8099bce06f37af83f07dc6dd38900c0e3db5edc30d2d54d__;!!Mih3wA!C6OKt9ppOgxUyXU_a7L_OyIzkMNiKHXdWTB7WZ_XJFMJo4py3zisEdFLteW_v0sBSHroF8xALoRl_sQ_nZ0MVotdD3JifA$" target="_blank" style="color:rgb(51,51,51)"><span style="color:rgb(96,96,96)">View in web browser</span></a>.</span></p>
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<p class="MsoNormal" style="margin:0in;font-size:11pt;font-family:Calibri,sans-serif"><span style="font-family:Arial,sans-serif;color:black"><a href="https://urldefense.com/v3/__https://www2.leica-microsystems.com/e/149501/2022-09-29/6j888k/1106960008?h=CmOza26exJ1UMTd7SOkd41Bvna2DealKEmuN5Ns8OuU__;!!Mih3wA!C6OKt9ppOgxUyXU_a7L_OyIzkMNiKHXdWTB7WZ_XJFMJo4py3zisEdFLteW_v0sBSHroF8xALoRl_sQ_nZ0MVovK3F21Ew$" style="color:rgb(51,51,51)"><span style="text-decoration-line:none"><img border="0" width="124" height="76" id="gmail-_x0000_i1025" src="http://www2.leica-microsystems.com/logo-lms"></span></a></span><span style="font-family:Arial,sans-serif"></span></p>
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<p class="MsoNormal" style="line-height:15pt;margin:0in;font-size:11pt;font-family:Calibri,sans-serif"><b><span style="font-size:15pt;font-family:Helvetica,sans-serif;color:rgb(80,80,80)"><img border="0" width="600" height="178" id="gmail-_x0000_i1026" src="https://www2.leica-microsystems.com/l/149501/2022-09-16/6h912n/149501/1663342487mK9mqcu1/Advanced_Cryo_Preparation_Techniques_Email_Banner_v2.png" style="border-style: solid; border-width: 0px; height: 178px; width: 600px;"></span></b></p>
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<h2 style="margin-right:0in;margin-left:0in;font-size:18pt;font-family:Calibri,sans-serif"><span style="font-family:Helvetica,sans-serif;color:rgb(80,80,80)">How to Prepare
Biological Samples for cryo-FIB Milling and cryo-EM</span></h2>
<h4 style="line-height:15pt;margin-right:0in;margin-left:0in;font-size:12pt;font-family:Calibri,sans-serif"><span style="font-family:Helvetica,sans-serif;color:rgb(80,80,80)">Tuesday 11th
October: 10:00 London | 11:00 Berlin | 13:00 Dubai</span></h4>
<p class="MsoNormal" style="line-height:15pt;margin:0in;font-size:11pt;font-family:Calibri,sans-serif"><span style="font-size:10.5pt;font-family:Helvetica,sans-serif;color:rgb(80,80,80)">Cryo-electron microscopy is becoming an important tool in
biomedical research, allowing researchers to explore the cellular landscape
in its native state and at high spatial resolution. A prerequisite to
obtaining that goal is the preparation of electron transparent samples
through cryo-FIB milling. This webinar highlights all steps in the
workflow, from capturing the live sample to the perfect cryo-EM
image. <br>
<br>
In this webinar, experts from Human Technopole and Leica Microsystems
will cover the different tools used to prepare cellular and biological
samples, from initial vitrification and high pressure freezing, towards
cryo-planing of bulk samples and confocal microscopy under cryo
conditions.<br>
<br>
A panel discussion will follow the presentations, during which our
experts will take questions from the audience.<br>
</span></p>
<p class="MsoNormal" align="center" style="text-align:center;line-height:15pt;margin:0in;font-size:11pt;font-family:Calibri,sans-serif"><span style="font-size:10.5pt;font-family:Arial,sans-serif;color:rgb(80,80,80)"><a href="https://urldefense.com/v3/__https://www2.leica-microsystems.com/e/149501/aration-techniques--ref-lms-e1/6j888n/1106960008?h=CmOza26exJ1UMTd7SOkd41Bvna2DealKEmuN5Ns8OuU__;!!Mih3wA!C6OKt9ppOgxUyXU_a7L_OyIzkMNiKHXdWTB7WZ_XJFMJo4py3zisEdFLteW_v0sBSHroF8xALoRl_sQ_nZ0MVouLdcPIjA$" style="color:rgb(51,51,51)"><span style="text-decoration-line:none"><img border="0" width="180" height="60" id="gmail-_x0000_i1027" src="https://www2.leica-microsystems.com/l/149501/2022-06-28/69x3lk/149501/1656430305wzZvuvAK/Register_now_button_1.png" style="border-style: solid; border-width: 0px; height: 60px; width: 180px;"></span></a></span></p>
<p class="MsoNormal" style="line-height:15pt;margin:0in;font-size:11pt;font-family:Calibri,sans-serif"><span style="font-size:10.5pt;font-family:Arial,sans-serif;color:rgb(80,80,80)"><br>
<strong>Also available on
demand - register your interest above.</strong></span></p>
<h4 style="line-height:15pt;margin-right:0in;margin-left:0in;font-size:12pt;font-family:Calibri,sans-serif"><span style="font-family:Helvetica,sans-serif;color:rgb(80,80,80)">Key Learnings:</span></h4>
<ul type="disc" style="margin-bottom:0in">
<li class="MsoNormal" style="color:rgb(80,80,80);line-height:15pt;margin:0in;font-size:11pt;font-family:Calibri,sans-serif"><span style="font-size:10.5pt;font-family:Helvetica,sans-serif">How to effectively
vitrify samples for on-grid lamella and lift-out procedures </span></li>
<li class="MsoNormal" style="color:rgb(80,80,80);line-height:15pt;margin:0in;font-size:11pt;font-family:Calibri,sans-serif"><span style="font-size:10.5pt;font-family:Helvetica,sans-serif">Cryo-planing methodology
for bulk samples</span></li>
<li class="MsoNormal" style="color:rgb(80,80,80);line-height:15pt;margin:0in;font-size:11pt;font-family:Calibri,sans-serif"><span style="font-size:10.5pt;font-family:Helvetica,sans-serif">Cryo-CLEM acquisition
routines and best practices</span></li>
<li class="MsoNormal" style="color:rgb(80,80,80);line-height:15pt;margin:0in;font-size:11pt;font-family:Calibri,sans-serif"><span style="font-size:10.5pt;font-family:Helvetica,sans-serif">Illustrations
of samples prepared via FIB milling</span></li>
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/ Panelists: </span></strong><span style="font-size:10.5pt;font-family:Helvetica,sans-serif;color:rgb(80,80,80)"></span></p>
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<p class="MsoNormal" style="margin:0in;font-size:11pt;font-family:Calibri,sans-serif"><span style="font-family:Arial,sans-serif"><img border="0" width="150" height="150" id="gmail-_x0000_i1028" src="https://www2.leica-microsystems.com/l/149501/2022-09-15/6h8k75/149501/1663258818ieEuvqQc/Philipp.jpg" style="border-style: solid; border-width: 0px; height: 150px; width: 150px;"></span></p>
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<p style="margin:12pt 0in;font-size:11pt;font-family:Calibri,sans-serif"><strong><span style="font-size:10.5pt;font-family:Arial,sans-serif;color:rgb(80,80,80)">Philipp Erdmann</span></strong><span style="font-size:10.5pt;font-family:Arial,sans-serif;color:rgb(80,80,80)"><br>
<em>Research Group Leader,
Erdmann Group, Human Technopole</em><br>
Philipp is a chemical biologist and microscopist. After five years as a
project group leader at the MPI for Biochemistry, Martinsried, he
joined HT in February 2021. Philipp’s lab focuses on the analysis of
liquid-liquid phase separation (LLPS) effects using in situ
cryo-electron tomography. LLPS is often involved in crucial processes
of both transmittable and non-transmittable human diseases, including
virus infections and neurodegenerative disorders. </span><span style="font-family:Arial,sans-serif"><br>
</span></p>
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<p class="MsoNormal" style="margin:0in;font-size:11pt;font-family:Calibri,sans-serif"><span style="font-family:Arial,sans-serif"><img border="0" width="151" height="151" id="gmail-_x0000_i1029" src="https://www2.leica-microsystems.com/l/149501/2022-09-15/6h8k78/149501/1663258837827IJva2/Malan.jpg" style="border-style: solid; border-width: 0px; height: 150px; width: 150px;"></span></p>
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<p style="margin:12pt 0in;font-size:11pt;font-family:Calibri,sans-serif"><strong><span style="font-size:10.5pt;font-family:Arial,sans-serif;color:rgb(80,80,80)">Malan Silva</span></strong><span style="font-size:10.5pt;font-family:Arial,sans-serif;color:rgb(80,80,80)"><br>
<em>Electron Microscopy
Specialist, Cryo-EM Facility, Human Technopole</em><br>
Malan is a structural neuroscientist specializing in cryo- and
plastic-section-based EM methods. Malan trained in ultramicrotomy and
electron microscopy, and in his postdoctoral studies he developed cryo
sectioning-based sample preparation pipelines to study the
ultrastructure of neurotransmission. Currently, at Human Technopole,
Malan is developing FIB-SEM-based sample preparation methods. Malan
plans to unify the cryo and room-temperature sample preparation methods
to maximize the scientific potential of existing technologies.</span><span style="font-family:Arial,sans-serif"></span></p>
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<p class="MsoNormal" style="margin:0in;font-size:11pt;font-family:Calibri,sans-serif"><span style="font-family:Arial,sans-serif"><img border="0" width="150" height="150" id="gmail-_x0000_i1030" src="https://www2.leica-microsystems.com/l/149501/2022-09-16/6h912v/149501/1663343070ngYFvoiy/fred.png" style="border-style: solid; border-width: 0px; height: 150px; width: 150px;"></span></p>
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<p class="MsoNormal" style="margin:0in;font-size:11pt;font-family:Calibri,sans-serif"><strong><span style="font-size:10.5pt;font-family:Arial,sans-serif;color:rgb(80,80,80)">Frédéric Leroux</span></strong><span style="font-size:10.5pt;font-family:Arial,sans-serif;color:rgb(80,80,80)"><br>
<em>Advanced Workflow
Manager, Leica Microsystems</em><br>
Frédéric completed his Master's degree in Biology at the University of
Ghent where he gained experience in biological EM sample preparation.
During his PhD at the EMAT research group University of Antwerp,
he specialized in advanced electron microscopy of composite materials,
going on to become EM sample preparation specialist at EMAT. In 2016,
he joined Leica Microsystems as Application Specialist Nanotechnology,
where he uses his multidisciplinary background and broad microscopy
experience to improve EM sample preparation for a variety of materials
(polymers, composites, biological and industrial materials).</span><span style="font-family:Arial,sans-serif"></span></p>
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<hr size="2" width="100%" align="center">
</span></div>
<p style="margin:9.75pt 0in 12pt;line-height:15pt;font-size:11pt;font-family:Calibri,sans-serif"><span style="font-size:10.5pt;font-family:Helvetica,sans-serif;color:rgb(80,80,80)">Best regards<br>
<br>
Your Leica Microsystems Nanotechnology Team</span></p>
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<p class="MsoNormal" style="margin:0in;font-size:11pt;font-family:Calibri,sans-serif"><img border="0" id="gmail-_x0000_i1039" src="https://www2.leica-microsystems.com/r/149501/1/1106960008/open/1"></p>
<p class="MsoNormal" style="margin:0in;font-size:11pt;font-family:Calibri,sans-serif"><img border="0" width="1" height="1" id="gmail-_x0000_i1040" src="https://pozkukj2.emltrk.com/v2/pozkukj2?d=%5bUNIQUE%5d"></p><br clear="all"><div><div dir="ltr" class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr"><font face="arial black, sans-serif">Dr. Andreia Lucia Pinto</font><div>Electron Microscopist</div><div>Advances Workflow Application Specialist @ Leica Microsystems</div><div><font size="1">Honorary Research Associate of the Imperial College London</font></div><div><font size="1">+49 (0) 1743132456 LinkedIn: Andreia Lucia do Nascimento Pinto</font><span style="font-size:x-small"> Twitter:@cellfie3</span></div><div><br></div></div></div></div></div>