[3dem] 3dem Digest, Vol 181, Issue 19
Cornelia Cazey
cornelia.cazey at cssb-hamburg.de
Thu Sep 15 00:10:53 PDT 2022
Hi David,
I am pretty sure our foam insulation is something like this L'isolante K-Flex https://urldefense.com/v3/__https://insulationandlagging.co.uk/Pipe-Insulation-Lagging/K-Flex-Class-0-Rubber-Pipe-Insulation__;!!Mih3wA!A9FrJvmLv7I_3SooGE51aBXBAdjA3473HRHM-RyWIWys8acD66VNGpOnpIT8n_YPqX0HvQEly80fop6fjv23xwpOrOsfFjp_E64$
Worth a try?
Best wishes,
Cornelia Cazey
Cryo-EM Facility
Centre for Structural Systems Biology (CSSB)
c/o DESY, Building 15
Notkestr. 85
22607 Hamburg
Germany
phone: +49-(0)40-8998-87706
email: cornelia.cazey at cssb-hamburg.de
"If you don't know it's impossible, it's easier to do" -Neil Gaiman
Occasionally I may send emails outside of general core working hours. I neither expect you to read, or respond outside of your own working hours!
Message: 4
Date: Wed, 14 Sep 2022 16:44:52 +0000
From: "Morgan, David Gene" <dagmorga at indiana.edu>
To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
Subject: [3dem] insulation for liquid nitrogen pipes?
Message-ID:
<MWHPR08MB2640549FC39B20F6E55A1157D0469 at MWHPR08MB2640.namprd08.prod.outlook.com>
Content-Type: text/plain; charset="iso-8859-1"
Hi,
Does anyone have a good source for foam (or similar material) insulation to wrap around pipes and hoses that carry liquid nitrogen. We got some through the university group that handles building care, but it tends to become very brittle when it is cold. It definitely insulates reasonably well, but if you move the insulated line, the insulation cracks and is beginning to fall apart.
This is completely different behavior from, for example, the insulation covering the hose that feeds liquid nitrogen into our Arctica. I haven't been able to determine what is used on the Arctica, but maybe someone can enlighten me.
Thanks for any suggestions you may have.
--
politics is more difficult than physics.
A. Einstein
David Gene Morgan
Electron Microscopy Center
047E Simon Hall
IU Bloomington
812 856 1457 (office)
812 856 3221 (3200)
https://urldefense.com/v3/__http://iubemcenter.indiana.edu__;!!Mih3wA!ELWcwS6s5tn8GcpqoD0poj3eZzYjRQaDreps1A8rvRKuIGqZz9HmNnIM1ICxj2MsJ4431kDRUsmlvyG0pDX1vf4$
On 14 Sep 2022, 21:00, at 21:00, 3dem-request at ncmir.ucsd.edu wrote:
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>Today's Topics:
>
> 1. Postdoctoral position in cryo-ET available in MRC-LMB
> (matteo allegretti)
> 2. Vacancy for a Scientific Programmer at EMDB (EMBL-EBI,
> Cambridge, UK) (Gerard Kleywegt)
> 3. Reminder: postdoc position in CryoEM, 21 September deadline
> (Per A Bullough)
> 4. insulation for liquid nitrogen pipes? (Morgan, David Gene)
>
>
>----------------------------------------------------------------------
>
>Message: 1
>Date: Wed, 14 Sep 2022 08:52:56 +0100
>From: "matteo allegretti" <matteoall at mrc-lmb.cam.ac.uk>
>To: 3dem at ncmir.ucsd.edu
>Subject: [3dem] Postdoctoral position in cryo-ET available in MRC-LMB
>Message-ID:
> <5cf6530bb071340ae768ab7b453d8629.squirrel at mail.mrc-lmb.cam.ac.uk>
>Content-Type: text/plain;charset=utf-8
>
>Dear colleagues,
>
>we are looking for a motivated postdoctoral candidate to join my group
>at
>MRC-LMB, Structural Studies Division
>(https://urldefense.com/v3/__https://www2.mrc-lmb.cam.ac.uk/group-leaders/a-to-g/matteo-allegretti/__;!!Mih3wA!EqYeMZtY3R3_gJaUml3c14he6LJGfQ20xjvrLQB3p0Hc2v_7vukIBnZOqUbxec8LOBQwFC5pLfii2JHuW4S6_kC2rVS8Tw$
> ).
>The candidate should be interested in establishing methods to study
>nuclear architecture in situ using cryo-FIB milling and electron cryo
>tomography and in vitro using single particle cryo-EM.
>
>Applicants must have a Ph.D. in life science, computer science or
>engineering (or due to complete within 6 months) and demonstrate the
>ability to have worked independently and in a team, to have a
>significant
>track record with original contributions, and to be committed to
>interdisciplinary, integrative research.
>
>The position is funded by the MRC, but the candidate can get my support
>in
>applying for fellowships.
>
>Applicants should provide a CV, a motivation letter including a brief
>description of current research activities and potential starting date,
>and contact information of at least 2 referees here:
>https://urldefense.com/v3/__https://mrc.tal.net/vx/mobile-0/appcentre-int/brand-3/candidate/so/pm/1/pl/3/opp/1942-Postdoctoral-Scientist-Structural-Studies-Dr-Matteo-Allegretti-LMB-1942/en-GB__;!!Mih3wA!EqYeMZtY3R3_gJaUml3c14he6LJGfQ20xjvrLQB3p0Hc2v_7vukIBnZOqUbxec8LOBQwFC5pLfii2JHuW4S6_kC1iXOPyQ$
>
>
>Application deadline is on the 9th of October. Please spread the voice
>and
>feel free to contact me (matteoall at mrc-lmb.cam.ac.uk) for more
>information
>regarding the positions.
>
>Cheers,
>Matteo
>
>
>
>--
>Matteo Allegretti
>Group leader MRC Laboratory of Molecular Biology
>Francis Crick Avenue, Cambridge Biomedical Campus
>Cambridge CB2 0QH, UK. Tel: +44 (0) 1223 26 7076
>https://urldefense.com/v3/__https://www2.mrc-lmb.cam.ac.uk/group-leaders/a-to-g/matteo-allegretti/__;!!Mih3wA!EqYeMZtY3R3_gJaUml3c14he6LJGfQ20xjvrLQB3p0Hc2v_7vukIBnZOqUbxec8LOBQwFC5pLfii2JHuW4S6_kC2rVS8Tw$
>
>
>
>
>------------------------------
>
>Message: 2
>Date: Wed, 14 Sep 2022 12:38:09 +0200 (CEST)
>From: Gerard Kleywegt <gerard at xray.bmc.uu.se>
>To: 3DEM Mailing List <3dem at ncmir.ucsd.edu>
>Cc: "Gerard Kleywegt \(EBI account\)" <gerard at ebi.ac.uk>
>Subject: [3dem] Vacancy for a Scientific Programmer at EMDB (EMBL-EBI,
> Cambridge, UK)
>Message-ID: <alpine.LRH.2.21.2209141237220.6783 at xray-mail.bmc.uu.se>
>Content-Type: text/plain; charset=US-ASCII; format=flowed
>
>
>Dear colleagues,
>
>EMBL-EBI is looking to recruit a Scientific Programmer to work on the
>Electron
>Microscopy Data Bank (EMDB; emdb-empiar.org), one of the three core
>archives of
>the Worldwide Protein Data Bank organisation (wwPDB; wwpdb.org).
>
>The Scientific Programmer will work in a small but tight international
>team
>focusing on managing the flow of data into the archive (data-in;
>deposition,
>validation and curation) and the public release of the data
>(production). In
>the past two years, EMDB has doubled in size to over 20,000 entries and
>the
>archive is set to continue to grow very rapidly in the years to come,
>so this
>is a very exciting time to join the effort.
>
>More information can be found in the vacancy notice at
>https://urldefense.com/v3/__https://www.embl.org/jobs/position/EBI02066__;!!Mih3wA!B7cYYQo2wEWJkbxyoZvcxSz8Ex0t8dqo9DsZK-lacg4wZvPD7eRsRLthy7mvVTbDbDxoMYet41m0p1h4YbNnHHaY$
>
>
>A detailed description of the role and its requirements is available
>from
>https://urldefense.com/v3/__https://oc.ebi.ac.uk/s/j1g7grHEJoeO9BJ__;!!Mih3wA!B7cYYQo2wEWJkbxyoZvcxSz8Ex0t8dqo9DsZK-lacg4wZvPD7eRsRLthy7mvVTbDbDxoMYet41m0p1h4YSUxeMX7$
>
>
>The application period for this post will close on 23 October 2022 and
>(virtual) interviews will take place on 17 or 18 November.
>
>Please feel free to bring this position to the attention of anyone with
>the
>right skillset who might be interested.
>
>Best wishes,
>
>Gerard Kleywegt
>
>---
>Gerard J. Kleywegt, EMBL-EBI, Hinxton, UK
>Head of Molecular and Cellular Structure
>gerard at ebi.ac.uk pdbe.org emdb-empiar.org
>PA: Roisin Dunlop pdbe_admin at ebi.ac.uk
>
>
>------------------------------
>
>Message: 3
>Date: Wed, 14 Sep 2022 13:28:23 +0100
>From: Per A Bullough <p.bullough at sheffield.ac.uk>
>To: 3DEM <3dem at ncmir.ucsd.edu>
>Subject: [3dem] Reminder: postdoc position in CryoEM, 21 September
> deadline
>Message-ID:
> <CA+hXxVV7Pp-ovn8V+N3a5=Kq3jdy=obtUkK9z7cu87Cvx6XAaQ at mail.gmail.com>
>Content-Type: text/plain; charset="utf-8"
>
>Applications are invited for a 4-year fixed term post-doctoral research
>position based in the laboratories of Prof Per Bullough (Biosciences),
>Dr
>Robert Fagan (Biosciences) and Prof. Jamie Hobbs (Physics and
>Astronomy) at
>The University of Sheffield. The project is funded by the BBSRC.
>
>In this multidisciplinary project you will join a team applying
>state-of-the-art techniques of structural and molecular biology to
>investigate the way spores of pathogenic bacteria germinate and emerge
>as
>metabolically active cells. For this first appointment we seek an
>expert in
>electron cryomicroscopy (CryoEM) and/or advanced protein X-ray
>crystallography. Experience in molecular biology techniques would be an
>advantage. In later stages of the project, we will also appoint an
>atomic
>force microscopist to join the project.
>
>We will study germination in two selected organisms- *Clostridium
>sporogenes* and *Clostridioides difficile*. *C. sporogenes *is
>non-pathogenic but very closely related to C. botulinum which causes
>botulism, a potentially lethal paralysis. *C. difficile* is the leading
>cause of antibiotic-associated diarrhoea, especially in hospital
>patients
>and the elderly.
>
>We will use CryoEM (cryotomography, single particle analysis and
>electron
>crystallography), AFM, fluorescence microscopy & molecular biology to
>understand how this remarkable, but little understood, metamorphosis
>takes
>place. We are superbly equipped for all this work, and we are part of a
>thriving community of enthusiastic and collaborative molecular
>microbiologists, structural biologists and biophysicists.
>
>Applicants are expected to have excellent interpersonal and
>communication
>skills, be highly independent and committed to research in a
>fast-moving
>and competitive field.
>
>The University of Sheffield is an internationally recognised centre of
>excellence in biology, with world-class research facilities. Research
>at
>Sheffield was ranked 4th in the UK for biological sciences and 1st in
>physics in the 2021 Research Excellence Framework. Sheffield is a
>vibrant
>and friendly city and incorporates part of the Peak District National
>Park
>within the city boundaries.
>
>For further details about the post, see
>https://urldefense.com/v3/__https://www.sheffield.ac.uk/jobs__;!!Mih3wA!C5dAcKAlSYLarrDt5vloTVuD5FSNz4Fu4CFn9XOrmEHuXUnfhVdaTG__bHp-oEP3sg91ubwy7qu5G4IprnoE51HawWTBwg$
> .
>The job reference is UOS034290.
>
>The deadline for applications is 21 September 2022.
>
>For an informal discussion about the position, please email me.
>
>Prof. Per A. Bullough
>Harrison Chair of Structural Biology
>Lead, Molecular Microbiology; Biochemistry to Disease
>School of Biosciences
>University of Sheffield
>Firth Court
>Western Bank
>Sheffield S10 2TN
>United Kingdom
>
>Tel: +44 (0)114 222 4245
>Fax: +44 (0)114 222 2800
>e-mail: p.bullough at sheffield.ac.uk <p.bullough at sheffield.ac.uk>
>https://urldefense.com/v3/__http://www.sheffield.ac.uk/mbb/staff/perbullough/perbullough__;!!Mih3wA!C5dAcKAlSYLarrDt5vloTVuD5FSNz4Fu4CFn9XOrmEHuXUnfhVdaTG__bHp-oEP3sg91ubwy7qu5G4IprnoE51Fdk2iszg$
>
>https://urldefense.com/v3/__http://www.imagine-imaginglife.com__;!!Mih3wA!C5dAcKAlSYLarrDt5vloTVuD5FSNz4Fu4CFn9XOrmEHuXUnfhVdaTG__bHp-oEP3sg91ubwy7qu5G4IprnoE51EfSplzLQ$
>
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>------------------------------
>
>Message: 4
>Date: Wed, 14 Sep 2022 16:44:52 +0000
>From: "Morgan, David Gene" <dagmorga at indiana.edu>
>To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
>Subject: [3dem] insulation for liquid nitrogen pipes?
>Message-ID:
> <MWHPR08MB2640549FC39B20F6E55A1157D0469 at MWHPR08MB2640.namprd08.prod.outlook.com>
>
>Content-Type: text/plain; charset="iso-8859-1"
>
>Hi,
>
>Does anyone have a good source for foam (or similar material)
>insulation to wrap around pipes and hoses that carry liquid nitrogen.
>We got some through the university group that handles building care,
>but it tends to become very brittle when it is cold. It definitely
>insulates reasonably well, but if you move the insulated line, the
>insulation cracks and is beginning to fall apart.
>
>This is completely different behavior from, for example, the insulation
>covering the hose that feeds liquid nitrogen into our Arctica. I
>haven't been able to determine what is used on the Arctica, but maybe
>someone can enlighten me.
>
>Thanks for any suggestions you may have.
>
>--
> politics is more difficult than physics.
> A. Einstein
>
> David Gene Morgan
> Electron Microscopy Center
> 047E Simon Hall
> IU Bloomington
> 812 856 1457 (office)
> 812 856 3221 (3200)
>https://urldefense.com/v3/__http://iubemcenter.indiana.edu__;!!Mih3wA!ELWcwS6s5tn8GcpqoD0poj3eZzYjRQaDreps1A8rvRKuIGqZz9HmNnIM1ICxj2MsJ4431kDRUsmlvyG0pDX1vf4$
>
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>
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>
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