<div dir="ltr"><div><div dir="ltr" class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr"><div style="font-family:Helvetica;font-size:14px;color:rgb(0,0,0)"><span style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small">Applications are invited for a 4-year fixed term post-doctoral research position based in the laboratories of Prof Per Bullough (Biosciences), Dr Robert Fagan (Biosciences) and Prof. Jamie Hobbs (Physics and Astronomy) at The University of Sheffield. The project is funded by the BBSRC.</span><br style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small"><br style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small"><span style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small">In this multidisciplinary project you will join a team applying state-of-the-art techniques of structural and molecular biology to investigate the way spores of pathogenic bacteria germinate and emerge as metabolically active cells. For this first appointment we seek an expert in electron cryomicroscopy (CryoEM) and/or advanced protein X-ray crystallography. Experience in molecular biology techniques would be an advantage. In later stages of the project, we will also appoint an atomic force microscopist to join the project.</span><br style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small"><span style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small"> </span><br style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small"><span style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small">We will study germination in two selected organisms- </span><i style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small">Clostridium sporogenes</i><span style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small"> and </span><i style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small">Clostridioides difficile</i><span style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small">. </span><i style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small">C. sporogenes </i><span style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small">is non-pathogenic but very closely related to C. botulinum which causes botulism, a potentially lethal paralysis. </span><i style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small">C. difficile</i><span style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small"> is the leading cause of antibiotic-associated diarrhoea, especially in hospital patients and the elderly.</span><br style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small"><span style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small"> </span><br style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small"><span style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small">We will use CryoEM (cryotomography, single particle analysis and electron crystallography), AFM, fluorescence microscopy & molecular biology to understand how this remarkable, but little understood, metamorphosis takes place. We are superbly equipped for all this work, and we are part of a thriving community of enthusiastic and collaborative molecular microbiologists, structural biologists and biophysicists.</span><br style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small"><span style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small"> </span><br style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small"><span style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small">Applicants are expected to have excellent interpersonal and communication skills, be highly independent and committed to research in a fast-moving and competitive field.</span><br style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small"><br style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small"><span style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small">The University of Sheffield is an internationally recognised centre of excellence in biology, with world-class research facilities. Research at Sheffield was ranked 4th in the UK for biological sciences and 1st in physics in the 2021 Research Excellence Framework. Sheffield is a vibrant and friendly city and incorporates part of the Peak District National Park within the city boundaries.</span><br style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small"><span style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small"> </span><br style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small"><span style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small">For further details about the post, see </span><a href="https://urldefense.com/v3/__https://www.sheffield.ac.uk/jobs__;!!Mih3wA!C5dAcKAlSYLarrDt5vloTVuD5FSNz4Fu4CFn9XOrmEHuXUnfhVdaTG__bHp-oEP3sg91ubwy7qu5G4IprnoE51HawWTBwg$" target="_blank" style="font-family:Arial,Helvetica,sans-serif;font-size:small">https://www.sheffield.ac.uk/jobs</a><span style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small">. The job reference is UOS034290.</span><br style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small"><br style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small"><span style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small">The deadline for applications is 21 September 2022.</span><br style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small"><br style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small"><span style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small">For an informal discussion about the position, please email me.</span><br></div><div style="font-family:Helvetica;font-size:14px;color:rgb(0,0,0)"><span style="color:rgb(34,34,34);font-family:Arial,Helvetica,sans-serif;font-size:small"><br></span></div><div style="font-family:Helvetica;font-size:14px;color:rgb(0,0,0)">Prof. Per A. Bullough</div><div style="font-family:Helvetica;font-size:14px;color:rgb(0,0,0)">Harrison Chair of Structural Biology</div><div style="font-family:Helvetica;font-size:14px;color:rgb(0,0,0)">Lead, Molecular Microbiology; Biochemistry to Disease<br>School of Biosciences<br>University of Sheffield<br>Firth Court<br>Western Bank<br>Sheffield S10 2TN<br>United Kingdom<br><br>Tel:      +44 (0)114 222 4245<br>Fax:      +44 (0)114 222 2800<br><a href="mailto:p.bullough@sheffield.ac.uk" target="_blank">e-mail:   p.bullough@sheffield.ac.uk</a><br><a href="https://urldefense.com/v3/__http://www.sheffield.ac.uk/mbb/staff/perbullough/perbullough__;!!Mih3wA!C5dAcKAlSYLarrDt5vloTVuD5FSNz4Fu4CFn9XOrmEHuXUnfhVdaTG__bHp-oEP3sg91ubwy7qu5G4IprnoE51Fdk2iszg$" target="_blank">http://www.sheffield.ac.uk/mbb/staff/perbullough/perbullough</a></div><div style="color:rgb(255,255,255);font-family:Helvetica;font-size:14px"><a href="https://urldefense.com/v3/__http://www.imagine-imaginglife.com/__;!!Mih3wA!C5dAcKAlSYLarrDt5vloTVuD5FSNz4Fu4CFn9XOrmEHuXUnfhVdaTG__bHp-oEP3sg91ubwy7qu5G4IprnoE51E6ZfVQzw$" target="_blank">http://www.imagine-imaginglife.com</a></div></div></div></div></div>