[3dem] EER home-made?

Takanori Nakane tnakane.protein at osaka-u.ac.jp
Thu Oct 27 06:37:41 PDT 2022


Hi,

Why do you want to do this?

EER is not an efficient format in terms of the compression ratio.

Also note that RELION's EER parser is hard coded for 4096 pixels and does not accept
other sizes.

Best regards,

Takanori Nakane

On 2022/10/27 22:25, Stahlberg Henning wrote:
> Hi,
> 
> Does anybody know if there is a public domain software available that can translate an EM image into a list of electron event recordings (EER file), 
> assuming the image is mostly black and has only a hand-full of electron impacts on it?
> 
> Obviously, that should at least run on a GPU, if not more specialized hardware. Dark-field, flat-field and PSF should be refined on the fly.
> 
> Henning.
> 
> *Henning Stahlberg*
> Laboratory of Biological Electron Microscopy
> Institute of Physics, School of Basic Sciences, EPFL, and
> Dep. of Fund. Microbiology, Faculty of Biology and Medicine, UNIL,
> Cubotron, BSP421, 1015 Lausanne, Switzerland
> lbem.epfl.ch 
> <https://urldefense.com/v3/__http://lbem.epfl.ch__;!!Mih3wA!EgSsjtPxILdQGBLYCe6YvOsUEdERiq0QhPq_T9khPOdej8-aPP_32UVqQGh1YUX8Nm__I_Zq2QW4B9Tll2iUskEiAQTVUQc$>
> 
> 
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