[3dem] Cancer therapeutics webinar utilizing Glacios 2 with Dr. Basil Greber : Tuesday, October 18 | 11 a.m. EDT | 8 a.m. PDT | 5 p.m. CEST

[Lengyel, Jeffrey]

H Everyone,

Please join us the upcoming Cancer therapeutics webinar with Dr. Basil Greber on Oct 18:


Dr. Basil Greber from the Institute of Cancer Research in London, UK, will demonstrate how the Glacios 2 Cryo-TEM facilitated the discovery of next-generation therapeutics.

Dr. Greber and his team used the Glacios 2 Cryo-TEM in their efforts to make CDK-activating kinase (CAK) amenable to structure-based drug design. First, they determined the structures of the human CAK bound to nucleotide analogues and two inhibitors. They then used the Glacios 2 Cryo-TEM equipped with a Thermo Scientific Selectris X Imaging Filter and a Thermo Scientific Falcon 4i Direct Electron Detector to uncover the structural basis of inhibitor selectivity, which required rapid acquisition of high-quality datasets for large numbers of different inhibitors.

This workflow enabled 3D reconstruction of the CAK at 3.5 to 4.5 Å resolution within one hour from the start of data collection and at approximately 3 Å resolution within four hours. Finally, they obtained more than a dozen high-resolution structures of the 85 kDa CDK-cyclin-module of the human CAK bound to inhibitors and nucleotides at up to 1.8 Å resolution using a Thermo Scientific Krios G4 Cryo-TEM with a cold-FEG. These structures provided detailed insight into inhibitor binding and suggested a mechanism for target selectivity that they are currently testing.
Register here!<https://urldefense.com/v3/__https://www.thermofisher.com/us/en/home/about-us/events/industrial/ls-cryo-electron-microscopy.html__;!!Mih3wA!EEE6zGi-C4b-te5dE-NPI0ZHWT-PeIotti1KYGGWnoK2FpqL_Q9-59VmA3_JMCSvpTW1vpIMg4nXKT3M27-rf7oU56KEbwtQBQ$  >

Best regards,

Jeff

____________________________________________________________________
Jeffrey Lengyel, Ph.D.
Director, Product Marketing, Cryo-EM, Electron Microscopy Life Sciences
Materials & Structural Analysis

Thermo Fisher Scientific
5350 NE Dawson Creek Drive | Hillsboro, OR 97124
Phone 503 560 9184
Jeffrey.Lengyel at thermofisher.com<mailto:Jeffrey.Lengyel at thermofisher.com>

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