[3dem] protein can't go to holes of cryo EM grids

[SSS Sasha]

Dear all,

I was trying to freeze cryo EM grids for a protein ~ 0.73MDa under several conditions, but it didn't work well. I make a brief summary here. Does anyone have any suggestions? Thanks in advance!

Holly grids CF-1.2/1.3-4Cu-50: plasma cleaning for 10s using O2,Ar2            Protein can't go in to the hole

Lacey carbon 400mesh Cu:  plasma cleaning for 15s using O2, Ar2        Contrast is quite low, hardly see the particles

GO on Quantifoil: plasma cleaning for 15s using H2, O2              Protein can't go into hole

Best regards,
Shasha
[cid:a307c715-3d34-42ee-a6a4-68e5b1269c9b]
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