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Dear all,</div>
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I was trying to freeze cryo EM grids for a protein ~ 0.73MDa under several conditions, but it didn't work well. I make a brief summary here. Does anyone have any suggestions? Thanks in advance!</div>
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Holly grids CF-1.2/1.3-4Cu-50: plasma cleaning for 10s using O2,Ar2 Protein can't go in to the hole</div>
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Lacey carbon 400mesh Cu: plasma cleaning for 15s using O2, Ar2 Contrast is quite low, hardly see the particles</div>
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GO on Quantifoil: <span style="background-color:rgb(255, 255, 255);display:inline !important" class="ContentPasted0">plasma cleaning for 15s using H2, O2 Protein can't go into hole</span></div>
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<span style="background-color:rgb(255, 255, 255);display:inline !important" class="ContentPasted0">Best regards,</span></div>
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<span style="background-color:rgb(255, 255, 255);display:inline !important" class="ContentPasted0">Shasha</span></div>
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