[3dem] model building from EM map

Guillaume Gaullier guillaume.gaullier at icm.uu.se
Fri May 20 01:26:39 PDT 2022


This also looks relevant:
Chojnowski G, Simpkin AJ, Leonardo DA, Seifert-Davila W, Vivas-Ruiz DE, Keegan RM & Rigden DJ (2022) findMySequence: a neural-network-based approach for identification of unknown proteins in X-ray crystallography and cryo-EM. IUCrJ 9 https://urldefense.proofpoint.com/v2/url?u=https-3A__doi.org_10.1107_S2052252521011088&d=DwIGaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=RElJcpwn-CtF99vEtQYc5eHucdQnP8-tJ3klX_YMFPMweYmHzZLOjH3XLf1qTPDo&s=hxhiujvaEmUp-30ouw4-R7VL_kWEXB4O0xTW0YU8Z5k&e= 

https://urldefense.proofpoint.com/v2/url?u=https-3A__gitlab.com_gchojnowski_findmysequence&d=DwIGaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=RElJcpwn-CtF99vEtQYc5eHucdQnP8-tJ3klX_YMFPMweYmHzZLOjH3XLf1qTPDo&s=TH2fxyQ7iAc1T_RZNTCdUFuHPiKy7uERPsCqUWyRF4w&e= 

I hope this helps,

Guillaume


On 19 May 2022, at 18:29, G.-Q. Hu <hu.guiqing at gmail.com<mailto:hu.guiqing at gmail.com>> wrote:

Thanks, I'll check it out.
Guiqing


On Thu, May 19, 2022 at 12:11 PM Dong Si <dongsi at uw.edu<mailto:dongsi at uw.edu>> wrote:
DeepTracer<https://urldefense.proofpoint.com/v2/url?u=https-3A__deeptracer.uw.edu_&d=DwMFaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=sLyOHl-maDMQxqcJ7rNK4O5JgOYKg77jIB5QEcYfIyn0CR7fzVpWApVipFfAJ6nD&s=WBWeHp9VykkYkyvZXs2Cq-5lAxiaeoR30dDmV6zREz4&e=> could help.

—
Dong Si, Ph.D.
faculty.washington.edu/dongsi<https://urldefense.proofpoint.com/v2/url?u=http-3A__faculty.washington.edu_dongsi&d=DwMFaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=sLyOHl-maDMQxqcJ7rNK4O5JgOYKg77jIB5QEcYfIyn0CR7fzVpWApVipFfAJ6nD&s=AMZ3TuChciYiG6mPyntCInMovZr5e1MWC6LQ7IZQWSA&e=>


On May 19, 2022, at 8:39 AM, G.-Q. Hu <hu.guiqing at gmail.com<mailto:hu.guiqing at gmail.com>> wrote:

Dear all,

I am a novice in atomic model building. There is  a small part of our EM map with ~20 residues which we do not know what it is. The EM map has the resolution 2.7 angstrom with side chains clearly visible.  I am wondering is there any way/software to predict the sequence from an EM map?

Thank you for your help!
Guiqing


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