[3dem] Apr 5 Webinar - Poliovirus and cryo-EM in biosafety labs

[Lavery, Leah]

All,

Please join us on Tuesday, April 5, 2022 at 9:00am (PDT),  12:00pm (EDT), 6:00pm (CEST) for a webinar by Dr. Selma Dahmane (Umea University) and Dr. Kristian Wadel (Thermo Fisher).  Kristian will introduce our cryo-EM 60C heat decontamination solution for higher BSL level labs (e.g. BSL3), and Selma will present her research on "Enterovirus membrane-assisted assembly and release revealed by cryo-electron tomography".

Webinar will be able to watch on-demand, but registration is required.  <https://urldefense.proofpoint.com/v2/url?u=https-3A__www.labroots.com_webinar_enterovirus-2Dmembrane-2Dassisted-2Dassembly-2Drelease-2Drevealed-2Dcryo-2Delectron-2Dtomography-3Fcampaign-3DLS-5FXMarket-5FGL-5FLabroots-2Demail-2DOFT-5FEmail-5F3rd-2DParty-5F2022-5F03&d=DwIFAg&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=NQ-c8CUnR_H63GXS04vkXj78uY817XCqxE-ZYk_BeLcJrnbNGHgrUcKnh2SO6p1A&s=kv1XCTr4m6Y3a3xcq5ALYwAbPcXBoxujdOwqohZy390&e= >
Register on Labroots.com: https://urldefense.proofpoint.com/v2/url?u=https-3A__www.labroots.com_webinar_enterovirus-2Dmembrane-2Dassisted-2Dassembly-2Drelease-2Drevealed-2Dcryo-2Delectron-2Dtomography&d=DwIFAg&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=NQ-c8CUnR_H63GXS04vkXj78uY817XCqxE-ZYk_BeLcJrnbNGHgrUcKnh2SO6p1A&s=zGVajJ_lhyZyUZzqq-LCRS3Vns6putoZqN8VTgMIqeo&e= 

Abstract
Enteroviruses are non-enveloped positive-sense RNA viruses that cause diverse diseases in humans. Their rapid multiplication depends on remodeling of cytoplasmic membranes for viral genome replication. It is unknown how virions assemble around these newly synthesized genomes and how they are then loaded into autophagic membranes for release through secretory autophagy. Here, we use cryo-electron tomography of infected cells to show that poliovirus assembles directly on replication membranes. Pharmacological untethering of capsids from membranes abrogates RNA encapsidation. Our data directly visualize a membrane-bound half-capsid as a prominent virion assembly intermediate. Assembly progression past this intermediate depends on the class III phosphatidylinositol 3-kinase VPS34, a key host-cell autophagy factor. On the other hand, the canonical autophagy initiator ULK1 is shown to restrict virion production since its inhibition leads to increased accumulation of virions in vast intracellular arrays, followed by an increased vesicular release at later time points. Finally, we identify multiple layers of selectivity in virus-induced autophagy, with a strong selection for RNA-loaded virions over empty capsids and the segregation of virions from other types of autophagosome contents. These findings provide an integrated structural framework for multiple stages of the poliovirus life cycle.
Learning Objectives

  *   Learn about 60C heat decontamination solution for higher BSL level labs (e.g. BSL3)
  *   Discuss how the poliovirus reshapes the membranes of infected cells during replication
  *   Visualize how virions assemble directly on replication membranes
  *   Reveal with in situ cryo-electron tomography the interplay between poliovirus and cellular autophagy

Leah Lavery, PhD
Sr. Marketing Development Manager, Thermo Fisher Scientific
https://urldefense.proofpoint.com/v2/url?u=https-3A__thermofisher.com_cryo-2Dem-2Dbiosafety&d=DwIFAg&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=NQ-c8CUnR_H63GXS04vkXj78uY817XCqxE-ZYk_BeLcJrnbNGHgrUcKnh2SO6p1A&s=VbNsgbm7UcCvUyOy5-ir3JlvU5eEuHUAnXt18GRQ3RQ&e= 



-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20220330/ec40d284/attachment.html>