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<p class="MsoNormal">All,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Please join us on Tuesday, April 5, 2022 at 9:00am (PDT), 12:00pm (EDT), 6:00pm (CEST) for a webinar by Dr. Selma Dahmane (Umea University) and Dr. Kristian Wadel (Thermo Fisher). Kristian will introduce our cryo-EM 60C heat decontamination
solution for higher BSL level labs (e.g. BSL3), and Selma will present her research on “<em><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#333333">Enterovirus membrane-assisted assembly and release revealed by cryo-electron tomography”</span></em><em><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#333333;font-style:normal">.
<o:p></o:p></span></em></p>
<p class="MsoNormal"><em><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#333333;font-style:normal"><o:p> </o:p></span></em></p>
<p class="MsoNormal"><em><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#333333;font-style:normal">Webinar will be able to watch on-demand, but registration is required. <a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__www.labroots.com_webinar_enterovirus-2Dmembrane-2Dassisted-2Dassembly-2Drelease-2Drevealed-2Dcryo-2Delectron-2Dtomography-3Fcampaign-3DLS-5FXMarket-5FGL-5FLabroots-2Demail-2DOFT-5FEmail-5F3rd-2DParty-5F2022-5F03&d=DwMFAg&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=NQ-c8CUnR_H63GXS04vkXj78uY817XCqxE-ZYk_BeLcJrnbNGHgrUcKnh2SO6p1A&s=kv1XCTr4m6Y3a3xcq5ALYwAbPcXBoxujdOwqohZy390&e="></a></span></em><em><span style="font-size:10.0pt;font-family:"Arial",sans-serif;font-style:normal"><o:p></o:p></span></em></p>
<p class="MsoNormal"><em><span style="font-size:10.0pt;font-family:"Arial",sans-serif;font-style:normal">Register on Labroots.com:
<a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__www.labroots.com_webinar_enterovirus-2Dmembrane-2Dassisted-2Dassembly-2Drelease-2Drevealed-2Dcryo-2Delectron-2Dtomography&d=DwMFAg&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=NQ-c8CUnR_H63GXS04vkXj78uY817XCqxE-ZYk_BeLcJrnbNGHgrUcKnh2SO6p1A&s=zGVajJ_lhyZyUZzqq-LCRS3Vns6putoZqN8VTgMIqeo&e=">
https://www.labroots.com/webinar/enterovirus-membrane-assisted-assembly-release-revealed-cryo-electron-tomography</a><o:p></o:p></span></em></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Abstract<o:p></o:p></p>
<p class="MsoNormal" style="text-indent:.5in">Enteroviruses are non-enveloped positive-sense RNA viruses that cause diverse diseases in humans. Their rapid multiplication depends on remodeling of cytoplasmic membranes for viral genome replication. It is unknown
how virions assemble around these newly synthesized genomes and how they are then loaded into autophagic membranes for release through secretory autophagy. Here, we use cryo-electron tomography of infected cells to show that poliovirus assembles directly on
replication membranes. Pharmacological untethering of capsids from membranes abrogates RNA encapsidation. Our data directly visualize a membrane-bound half-capsid as a prominent virion assembly intermediate. Assembly progression past this intermediate depends
on the class III phosphatidylinositol 3-kinase VPS34, a key host-cell autophagy factor. On the other hand, the canonical autophagy initiator ULK1 is shown to restrict virion production since its inhibition leads to increased accumulation of virions in vast
intracellular arrays, followed by an increased vesicular release at later time points. Finally, we identify multiple layers of selectivity in virus-induced autophagy, with a strong selection for RNA-loaded virions over empty capsids and the segregation of
virions from other types of autophagosome contents. These findings provide an integrated structural framework for multiple stages of the poliovirus life cycle.<o:p></o:p></p>
<p class="MsoNormal"><o:p></o:p></p>
<p class="MsoNormal">Learning Objectives<o:p></o:p></p>
<ul style="margin-top:0in" type="disc">
<li class="MsoListParagraph" style="margin-left:0in;mso-list:l1 level1 lfo2">Learn about 60C heat decontamination solution for higher BSL level labs (e.g. BSL3)<o:p></o:p></li><li class="MsoListParagraph" style="margin-left:0in;mso-list:l1 level1 lfo2">Discuss how the poliovirus reshapes the membranes of infected cells during replication<o:p></o:p></li><li class="MsoListParagraph" style="margin-left:0in;mso-list:l1 level1 lfo2">Visualize how virions assemble directly on replication membranes<o:p></o:p></li><li class="MsoListParagraph" style="margin-left:0in;mso-list:l1 level1 lfo2">Reveal with in situ cryo-electron tomography the interplay between poliovirus and cellular autophagy<o:p></o:p></li></ul>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal" style="background:white"><b><span style="font-size:9.0pt;font-family:"Arial",sans-serif;color:#555759;background:white">Leah Lavery, PhD</span></b><span lang="EN-GB"><o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:9.0pt;font-family:"Arial",sans-serif;color:#555759;background:white">Sr. Marketing Development Manager, Thermo Fisher Scientific</span><span lang="EN-GB"><o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><u><span style="font-size:9.0pt;font-family:"Arial",sans-serif;color:#0563C1;background:white"><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__thermofisher.com_cryo-2Dem-2Dbiosafety&d=DwQFAg&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=NQ-c8CUnR_H63GXS04vkXj78uY817XCqxE-ZYk_BeLcJrnbNGHgrUcKnh2SO6p1A&s=VbNsgbm7UcCvUyOy5-ir3JlvU5eEuHUAnXt18GRQ3RQ&e=">https://thermofisher.com/cryo-em-biosafety</a></span></u><span lang="EN-GB"><o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="color:black;background:white"> </span><span lang="EN-GB"><o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
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