[3dem] tilted transfer functions again

Michael Elbaum michael.elbaum at weizmann.ac.il
Sat Mar 5 22:15:59 PST 2022


Hi David,    I remembered this paper from 2007 in Ultramicroscopy, "The contrast-imaging function for tilted specimens" (doi:10.1016/j.ultramic.2006.07.010). seems relevant to your question!

best wishes,
Michael

________________________________
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Morgan, David Gene <dagmorga at indiana.edu>
Sent: Friday, March 4, 2022 7:23:40 PM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] tilted transfer functions again

Hi,

Thanks to all for their input.  Prodded by several of you, I realized that I was trying to remember a program called ctftilt that was release in parallel with ctffind3 many years ago.

>From the responses I received, it appears that goCTF and the version of ctffind in the development branch of cisTEM are more recent versions that do what ctftilt did.

It's also clear that some packages have built-in ways to process single particle data acquired from a tilted grid.  I was looking for something to evaluate the images themselves, before doing any further processing.  I did not try any of these packages, and it is entirely possible that they generate diagnostic data similar to the results from ctftilt (and the others listed above).

Again, thanks for all your input, and I hope this summary isn't a waste of your time.

--
    politics is more difficult than physics.
                                             A. Einstein

            David Gene Morgan
        Electron Microscopy Center
             047E Simon Hall
             IU Bloomington
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          812 856 3221 (3200)
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