[3dem] Postdoctoral position in the Cryo-EM laboratory of Dr. Christoper Akey

Tue Jan 25 06:58:55 PST 2022

Structural Cryo-EM community -

The Akey laboratory in the Department of Physiology and Biophysics at Boston University School of Medicine has an open position for a post-doctoral associate in cryo-electron microscopy funded by an NIH grant for 3 years. Research in the group is focused on the structure and function of protein translocation machines, including the yeast Nuclear Pore Complex (NPC) and bacterial ribosome-SecY channel complexes. In these studies, 3D single particle and tomographic, sub-volume averaging methods are used to elucidate the structures of  these remarkable translocation machines: For an overview please see:  (https://urldefense.proofpoint.com/v2/url?u=https-3A__www.bumc.bu.edu_phys-2Dbiophys_profile_christopher-2Dakey_&d=DwIGaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=yrIy-S1h3O-GXr739q8qKMz5FihFtYTWQXS4zSm5MDkNnjW3P-9q3yc57rmc-rla&s=GBFRt4bg76JtC7RP8RzXix-vxphdhZoZH7OjeLMNxtY&e= ).

Our research is highly collaborative and once suitable samples are prepared at our cryo-EM facility, data are collected remotely at National cryo-EM beamlines for analysis on our groups' in-house gpu-cpu cluster. The successful candidate will have a Ph.D. with expertise in Biophysics, Computational or Structural Biology; prior experience in cryo-EM, while a plus, is not required as extensive training will be provided.

To apply, please send a cover letter, CV and contact information for 3 references to: cakey at bu.edu.

Recent publications from the NPC consortium and a highlight:

Akey CW, Singh D, Ouch C, Echeverria I, Nudelman I, Varberg JM, Yu Z, Fang F, Shi Y, Wang J, Salzberg D, Song K, Xu C, Gumbart JC, Suslov S, Unruh J, Jaspersen SL, Chait BT, Sali A, Fernandez-Martinez J, Ludtke SJ, Villa E, Rout MP. (2022). Comprehensive structure and functional adaptations of the yeast nuclear pore complex. Cell 185:361-378.

Preview: Megan R. McCarthy and C. Patrick Lusk (2022). One ring doesn’t rule them all: Distinct nuclear pore complexes in a single cell. https://urldefense.proofpoint.com/v2/url?u=https-3A__www.sciencedirect.com_science_article_pii_S0092867421015646-3Fdgcid-3Dcoauthor&d=DwIGaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=yrIy-S1h3O-GXr739q8qKMz5FihFtYTWQXS4zSm5MDkNnjW3P-9q3yc57rmc-rla&s=g6I1Wfz6q5os12EcyJAfo98lNRm3qtXLZpxoJXAaRUE&e= 

Kim SJ, Fernandez-Martinez J, Nudelman I, Shi Y, Zhang W, Raveh B, Herricks T, Slaughter BD, Hogan JA, Upla P, Chemmama IE, Pellarin R, Echeverria I, Shivaraju M, Chaudhury AS, Wang J, Williams R, Unruh JR, Greenberg CH, Jacobs EY, Yu Z, de la Cruz MJ, Mironska R, Stokes DL, Aitchison JD, Jarrold MF, Gerton JL, Ludtke SJ, Akey CW, Chait BT, Sali A, Rout MP. (2018) Integrative structure and functional anatomy of a nuclear pore complex. Nature 555:475-482.

Akey research group:

Cheng, T.C., Akey, I.V., Yuan, S., Yu, Z., Ludtke, S.J. and Akey, C.W. (2017). A near atomic structure of the Dark apoptosome provides insight into assembly and activation. Structure 25:40-52. doi: 10.1016/j.str.2016.11.002. PMID: 27916517

Cheng, T.C., Hong, C., Akey, I.V., Yuan, S. and Akey, C.W. (2016). A near atomic structure of the active human apoptosome. Elife. Oct 4;5. pii: e17755. doi: 10.7554/eLife.17755. PMID: 27697150

Park, E., Ménétret, J. F., Gumbart, J.C., Ludtke, S.J., Li, W., Whynot, A., Rapoport, T. A., and Akey, C. W. (2014). Structure of the SecY channel during initiation of protein translocation. Nature 506, 102-106. PMID: 24153188

Pubmed:https://urldefense.proofpoint.com/v2/url?u=https-3A__pubmed.ncbi.nlm.nih.gov_-3Fterm-3Dakey-2Bcw-26sort-3Ddate-26size-3D100https-3A__pubmed.ncbi.nlm.nih.gov_-3Fterm-3Dakey-2Bcw-26sort-3Ddate-26size-3D100&d=DwIGaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=yrIy-S1h3O-GXr739q8qKMz5FihFtYTWQXS4zSm5MDkNnjW3P-9q3yc57rmc-rla&s=H7oDDx7t6_LV_QjCpGBHs_6OJ5XW9Fq0tDvkSB4iK1c&e= <https://urldefense.proofpoint.com/v2/url?u=https-3A__pubmed.ncbi.nlm.nih.gov_-3Fterm-3Dakey-2Bcw-26sort-3Ddate-26size-3D100&d=DwIGaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=yrIy-S1h3O-GXr739q8qKMz5FihFtYTWQXS4zSm5MDkNnjW3P-9q3yc57rmc-rla&s=EQPT0h64N2Gtz35cKQLI1c8295OCO5msJ_8OyNXLqZA&e= >

cheers,

C Akey
Prof.

----------------------------------------------------------------------

            Structural Electron Microscopy Group
----------------------------------------------------------------------
Christopher W. Akey, Professor
Dept. of Physiology and Biophysics
Boston Univ. School of Medicine
700 Albany St.
Boston, Mass. 02118-2526

office: 617 358-8451 lab: 8-8450 Computer room: 8-8458
 cakey at bu.edu
https://urldefense.proofpoint.com/v2/url?u=https-3A__www.bumc.bu.edu_phys-2Dbiophys_profile_christopher-2Dakey_&d=DwIGaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=yrIy-S1h3O-GXr739q8qKMz5FihFtYTWQXS4zSm5MDkNnjW3P-9q3yc57rmc-rla&s=GBFRt4bg76JtC7RP8RzXix-vxphdhZoZH7OjeLMNxtY&e= 

-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20220125/fbebb6ab/attachment.html>