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<p style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:"Times New Roman""><span style="font-size:11.0pt;font-family:Calibri;mso-bidi-font-family:Arial">Structural Cryo-EM community -

<o:p> </o:p></span></p>

<p style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:"Times New Roman""><span style="font-size:11.0pt;font-family:Calibri;mso-bidi-font-family:Arial"><o:p> </o:p></span></p>

<p style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:"Times New Roman""><span style="font-size:11.0pt;font-family:Calibri;mso-bidi-font-family:Arial">The Akey laboratory in the Department of Physiology and Biophysics at Boston University School of Medicine

 has an open position for a post-doctoral associate in cryo-electron microscopy funded by an NIH grant for 3 years. Research in the group is focused on the structure and function of protein translocation machines, including the yeast Nuclear Pore Complex (NPC)

 and bacterial ribosome-SecY channel complexes. In these studies, 3D single particle and tomographic, sub-volume averaging methods are used to elucidate the structures of

<span style="mso-spacerun:yes"> </span>these remarkable translocation machines: For an overview please see:<span style="mso-spacerun:yes"> 

</span>(<span style="color:blue"><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__www.bumc.bu.edu_phys-2Dbiophys_profile_christopher-2Dakey_&d=DwMGaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=yrIy-S1h3O-GXr739q8qKMz5FihFtYTWQXS4zSm5MDkNnjW3P-9q3yc57rmc-rla&s=GBFRt4bg76JtC7RP8RzXix-vxphdhZoZH7OjeLMNxtY&e=">https://www.bumc.bu.edu/phys-biophys/profile/christopher-akey/</a></span>).<o:p> </o:p></span></p>

<p style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:"Times New Roman""><span style="font-size:7.0pt;font-family:Calibri;mso-bidi-font-family:Arial"><o:p> </o:p></span></p>

<p style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:"Times New Roman""><span style="font-size:11.0pt;font-family:Calibri;mso-bidi-font-family:Arial">Our research is highly collaborative and once suitable samples are prepared at our cryo-EM facility,

 data are collected remotely at National cryo-EM beamlines for analysis on our groups' in-house gpu-cpu cluster. The successful candidate will have a Ph.D. with expertise in Biophysics, Computational or Structural Biology; prior experience in cryo-EM, while

 a plus, is not required as extensive training will be provided.<o:p> </o:p></span></p>

<p style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:"Times New Roman""><span style="font-size:7.0pt;font-family:Calibri;mso-bidi-font-family:Arial"><o:p> </o:p></span></p>

<p style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:"Times New Roman""><span style="font-size:11.0pt;font-family:Calibri;mso-bidi-font-family:Arial">To apply, please send a cover letter, CV and contact information for 3 references to:

<span style="color:blue">cakey@bu.edu</span>.<o:p> </o:p></span></p>

<p style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:"Times New Roman""><span style="font-size:7.0pt;font-family:Calibri;mso-bidi-font-family:Arial"><o:p> </o:p></span></p>

<p style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:"Times New Roman""><span style="font-size:11.0pt;font-family:Calibri;mso-bidi-font-family:Arial">Recent publications from the

<i>NPC consortium and a highlight:</i> <o:p> </o:p></span></p>

<p style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:"Times New Roman";margin-top:0in;margin-right:4.5pt;margin-bottom:.0001pt;margin-left:4.5pt;tab-stops:-135.0pt -45.0pt 31.0pt 1.25in">

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<b><span style="font-size:11.0pt;font-family:Calibri;mso-bidi-font-family:Arial">Akey CW</span></b><span style="font-size:11.0pt;font-family:Calibri;mso-bidi-font-family:Arial">, Singh D, Ouch C, Echeverria I, Nudelman I, Varberg JM, Yu Z, Fang F, Shi Y, Wang

J, Salzberg D, Song K, Xu C, Gumbart JC, Suslov S, Unruh J, Jaspersen SL, Chait BT, Sali A, Fernandez-Martinez J, Ludtke SJ, Villa E, Rout MP. (2022). Comprehensive structure and functional adaptations of the yeast nuclear pore complex. Cell 185:361-378.

<o:p> </o:p></span></p>

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<span style="font-size:6.0pt;font-family:Calibri;mso-bidi-font-family:Arial"><o:p> </o:p></span></p>

<p style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:"Times New Roman";mso-layout-grid-align:none">

<span style="font-size:11.0pt;font-family:Calibri;mso-bidi-font-family:Arial">Preview: Megan R. McCarthy and C. Patrick Lusk (2022).

<i>One ring doesn’t rule them all: Distinct nuclear pore complexes in a single cell.</i><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__www.sciencedirect.com_science_article_pii_S0092867421015646-3Fdgcid-3Dcoauthor&d=DwMGaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=yrIy-S1h3O-GXr739q8qKMz5FihFtYTWQXS4zSm5MDkNnjW3P-9q3yc57rmc-rla&s=g6I1Wfz6q5os12EcyJAfo98lNRm3qtXLZpxoJXAaRUE&e=" target="_blank"> https://www.sciencedirect.com/science/article/pii/S0092867421015646?dgcid=coauthor</a><o:p> </o:p></span></p>

<p style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:"Times New Roman";margin-right:4.5pt;tab-stops:-135.0pt -45.0pt 31.0pt 1.25in">

<span style="font-family:Calibri;mso-bidi-font-family:Arial"><o:p> </o:p></span></p>

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<span style="font-size:11.0pt;font-family:Calibri;mso-fareast-font-family:ArialMT;mso-bidi-font-family:ArialMT;color:black">Kim SJ</span><span style="font-size:11.0pt;font-family:Calibri;mso-fareast-font-family:ArialMT;mso-bidi-font-family:Arial-BoldMT;color:black;mso-bidi-font-weight:bold">,

 Fernandez-Martinez J</span><span style="font-size:11.0pt;font-family:Calibri;mso-fareast-font-family:ArialMT;mso-bidi-font-family:ArialMT;color:black">, Nudelman I, Shi Y, Zhang W, Raveh B, Herricks T, Slaughter BD, Hogan JA,

</span><span style="font-size:11.0pt;font-family:Calibri;mso-fareast-font-family:ArialMT;mso-bidi-font-family:Arial-BoldMT;color:black;mso-bidi-font-weight:bold">Upla P</span><span style="font-size:11.0pt;font-family:Calibri;mso-fareast-font-family:ArialMT;mso-bidi-font-family:ArialMT;color:black">,

 Chemmama IE, Pellarin R</span><span style="font-size:11.0pt;font-family:Calibri;mso-fareast-font-family:ArialMT;mso-bidi-font-family:Arial-BoldMT;color:black;mso-bidi-font-weight:bold">, Echeverria I</span><span style="font-size:11.0pt;font-family:Calibri;mso-fareast-font-family:ArialMT;mso-bidi-font-family:ArialMT;color:black">,

 Shivaraju M, Chaudhury AS, Wang J, Williams R, Unruh JR, Greenberg</span><span style="font-size:11.0pt;font-family:Calibri;mso-fareast-font-family:ArialMT;mso-bidi-font-family:Arial-BoldMT;color:black;mso-bidi-font-weight:bold">

</span><span style="font-size:11.0pt;font-family:Calibri;mso-fareast-font-family:ArialMT;mso-bidi-font-family:ArialMT;color:black">CH, Jacobs EY, Yu Z, de la Cruz MJ, Mironska R, Stokes DL, Aitchison JD, Jarrold MF, Gerton JL,

</span><span style="font-size:11.0pt;font-family:Calibri;mso-fareast-font-family:ArialMT;mso-bidi-font-family:Arial-BoldMT;color:black;mso-bidi-font-weight:bold">Ludtke SJ</span><span style="font-size:11.0pt;font-family:Calibri;mso-fareast-font-family:ArialMT;mso-bidi-font-family:ArialMT;color:black">,

</span><b><span style="font-size:11.0pt;font-family:Calibri;mso-fareast-font-family:ArialMT;mso-bidi-font-family:Arial-BoldMT;color:black">Akey CW</span></b><span style="font-size:11.0pt;font-family:Calibri;mso-fareast-font-family:ArialMT;mso-bidi-font-family:Arial-BoldMT;color:black;mso-bidi-font-weight:bold">,

 Chait BT</span><span style="font-size:11.0pt;font-family:Calibri;mso-fareast-font-family:ArialMT;mso-bidi-font-family:ArialMT;color:black">,

</span><span style="font-size:11.0pt;font-family:Calibri;mso-fareast-font-family:ArialMT;mso-bidi-font-family:Arial-BoldMT;color:black;mso-bidi-font-weight:bold">Sali A</span><span style="font-size:11.0pt;font-family:Calibri;mso-fareast-font-family:ArialMT;mso-bidi-font-family:ArialMT;color:black">,

<span style="font-size:11.0pt;font-family:Calibri;mso-fareast-font-family:ArialMT;mso-bidi-font-family:Arial-BoldMT;color:black;mso-bidi-font-weight:bold">Rout MP<span style="font-size:11.0pt;font-family:Calibri;mso-fareast-font-family:ArialMT;mso-bidi-font-family:ArialMT;color:black">.

(2018) Integrative structure and functional anatomy of a nuclear pore complex. Nature 555:475-482.

<o:p> </o:p></span></p>

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<i><span style="font-size:6.0pt;font-family:Calibri;mso-bidi-font-family:Arial"><o:p> </o:p></span></i></p>

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<i><span style="font-size:11.0pt;font-family:Calibri;mso-bidi-font-family:Arial">Akey research group:</span></i><span style="font-size:11.0pt;font-family:Calibri;mso-fareast-font-family:ArialMT;mso-bidi-font-family:Arial-BoldMT;color:black;mso-bidi-font-weight:bold"><o:p>

<br>

</o:p></span></p>

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<span style="font-size:11.0pt;font-family:Calibri;mso-fareast-font-family:ArialMT;mso-bidi-font-family:Arial-BoldMT;color:black;mso-bidi-font-weight:bold"><o:p><br>

</o:p></span></p>

<p style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:"Times New Roman";mso-margin-top-alt:auto;margin-right:4.5pt;mso-margin-bottom-alt:auto">

<span style="font-size:11.0pt;font-family:Calibri">Cheng, T.C., Akey, I.V., Yuan, S., Yu, Z., Ludtke, S.J. and

Akey, C.W. (2017). A near atomic structure of the Dark apoptosome provides insight into assembly and activation. Structure 25:40-52. doi: 10.1016/j.str.2016.11.002.

<st1:stockticker>PMID</st1:stockticker>: 27916517</span><i><span style="font-size:11.0pt;font-family:Calibri;mso-bidi-font-family:Arial"><o:p>

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</o:p></span></i></p>

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<i><span style="font-size:11.0pt;font-family:Calibri;mso-bidi-font-family:Arial"><o:p><br>

</o:p></span></i></p>

<p style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:"Times New Roman";mso-margin-top-alt:auto;margin-right:4.5pt;mso-margin-bottom-alt:auto;tab-stops:4.5pt">

<span style="font-size:11.0pt;font-family:Calibri;mso-bidi-font-family:Arial">Cheng, T.C., Hong, C., Akey, I.V., Yuan, S. and

Akey, C.W. (2016). A near atomic structure of the active human apoptosome.

</i>Elife. Oct 4;5. pii: e17755. doi: 10.7554/eLife.17755. <st1:stockticker>PMID</st1:stockticker>: 27697150<o:p>

<br>

</o:p></span></p>

<p style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:"Times New Roman";mso-margin-top-alt:auto;margin-right:4.5pt;mso-margin-bottom-alt:auto;tab-stops:4.5pt">

<span style="font-size:11.0pt;font-family:Calibri;mso-bidi-font-family:Arial"><o:p><br>

</o:p></span></p>

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<span style="font-size:11.0pt;font-family:Calibri;mso-bidi-font-family:Arial">Park, E., Ménétret, J. F., Gumbart, J.C., Ludtke, S.J., Li, W., Whynot, A., Rapoport, T. A., and

Akey, C. W. (2014). Structure of the SecY channel during initiation of protein translocation. Nature 506, 102-106.

<st1:stockticker>PMID</st1:stockticker>: 24153188<o:p> </o:p></span></p>

<p style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:"Times New Roman""><span style="font-size:6.0pt;font-family:Calibri;mso-bidi-font-family:Arial"><o:p> </o:p></span></p>

<p style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:"Times New Roman""><span style="font-size:11.0pt;font-family:Calibri;mso-bidi-font-family:Arial">Pubmed:<span style="color:blue"><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__pubmed.ncbi.nlm.nih.gov_-3Fterm-3Dakey-2Bcw-26sort-3Ddate-26size-3D100&d=DwMGaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=yrIy-S1h3O-GXr739q8qKMz5FihFtYTWQXS4zSm5MDkNnjW3P-9q3yc57rmc-rla&s=EQPT0h64N2Gtz35cKQLI1c8295OCO5msJ_8OyNXLqZA&e=">https://pubmed.ncbi.nlm.nih.gov/?term=akey+cw&sort=date&size=100https://pubmed.ncbi.nlm.nih.gov/?term=akey+cw&sort=date&size=100</a></span><o:p> </o:p></span></p>

<p style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:"Times New Roman""><span style="font-size:11.0pt;font-family:Calibri;mso-bidi-font-family:Arial"><o:p></o:p></span></p>

<p style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:"Times New Roman""><span style="font-size:11.0pt;font-family:Calibri;mso-bidi-font-family:Arial"><o:p></o:p></span></p>

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cheers,</div>

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C Akey</div>

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Prof.</div>

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<span style="font-size:11.0pt">      <span style="">    </span>  <span style="font-size:12pt; font-family:Calibri,Helvetica,sans-serif">

Structural Electron Microscopy</span></span><span style="font-size:12pt; font-family:Calibri,Helvetica,sans-serif"> Group</span><span style="font-size:11.0pt"><br>

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</span><span style="font-size:11pt; font-family:Calibri,Helvetica,sans-serif">Christopher W. Akey, Professor</span><span style="font-size:11.0pt"><br>

</span><span style="font-size:11pt; font-family:Calibri,Helvetica,sans-serif">Dept. of Physiology and Biophysics</span><span style="font-size:11.0pt"><br>

</span><span style="font-size:11pt; font-family:Calibri,Helvetica,sans-serif">Boston Univ. School of Medicine</span><span style="font-size:11.0pt"><br>

</span><span style="font-size:11pt; font-family:Calibri,Helvetica,sans-serif">700 Albany St.</span><span style="font-size:11.0pt"><br>

</span><span style="font-size:11pt; font-family:Calibri,Helvetica,sans-serif">Boston, Mass. 02118-2526</span><span style="font-size:11.0pt"><br>

</span><span style="font-size:9pt"></span><span style="font-size:11.0pt"><br>

</span><span style="font-size:11pt; font-family:Calibri,Helvetica,sans-serif">office: 617 358-8451 lab: 8-8450 Computer room: 8-8458</span><span style="font-size:11.0pt"><br>

<span style="font-family:Calibri,Helvetica,sans-serif"> </span></span><span style="font-size:11pt; font-family:Calibri,Helvetica,sans-serif">cakey@bu.edu</span><span style="font-size:11.0pt"><br>

<span style="color:blue; font-family:Calibri,Helvetica,sans-serif"><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__www.bumc.bu.edu_phys-2Dbiophys_profile_christopher-2Dakey_&d=DwQGaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=yrIy-S1h3O-GXr739q8qKMz5FihFtYTWQXS4zSm5MDkNnjW3P-9q3yc57rmc-rla&s=GBFRt4bg76JtC7RP8RzXix-vxphdhZoZH7OjeLMNxtY&e=">https://www.bumc.bu.edu/phys-biophys/profile/christopher-akey/</a></span><span style="color:blue"><span style="font-family:Calibri,Helvetica,sans-serif"> </span></span></span></p>

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