[3dem] Refining polished particles

[Sjors Scheres - MRC LMB]

Dear Jay,

The half-sets are still separated in polishing, so iterative overfitting
will be avoided. Just use the particles (data.star) from Refine3D and
the postprocessing STAR file from your best refinement. The higher
resolution, the better, but 4A would also do.

HTH,

Sjors


On 1/9/22 2:29 AM, Jay Rai wrote:
> Dear all,
> Can we use 4 angstroms reference map during refinement of polish
> particles? Also can we also do only local alignment/refinement only if
> the particles before polishing yield 2.5 angstroms ( with each
> unfiltered half maps are 3.5 angstroms).
> Do you think it will be a model bias?  
> Any suggestions or thought are appreciated.
>
> With kind regards,
> Jay
>
>
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-- 
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
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