[3dem] [External] Re: tilted transfer functions?

[Morgan, David Gene]

Daniel,

The problem is that the tilt causes the higher resolution Thon rings to become out-of-phase, and so the standard programs can't give a decent estimate of the goodness of-fit.  I vaguely remember that someone had a program that took the tilt into account a number of years ago, but a quick hunt for it didn't turn up anything.

--
    politics is more difficult than physics.
                                             A. Einstein

            David Gene Morgan
        Electron Microscopy Center
             047E Simon Hall
             IU Bloomington
          812 856 1457 (office)
          812 856 3221 (3200)
      https://urldefense.proofpoint.com/v2/url?u=http-3A__iubemcenter.indiana.edu&d=DwIFAg&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=XfGX1GAOnfj3mLhTnyN8uolpwG3BMtMXZYm0H1sLXZy6LYulpNRf8T8jKuxWTOju&s=qBVmiqzIFHPhsmMv2GVKS3tE0Y3k35YCkiZM5CmZ2cg&e= 
________________________________
From: Daniel Asarnow <asarnow at msg.ucsf.edu>
Sent: Monday, February 28, 2022 5:45 PM
To: Morgan, David Gene <dagmorga at indiana.edu>
Cc: 3dem at ncmir.ucsd.edu <3dem at ncmir.ucsd.edu>
Subject: [External] Re: [3dem] tilted transfer functions?

This message was sent from a non-IU address. Please exercise caution when clicking links or opening attachments from external sources.

I think any patch-based CTF program, like the one in cryosparc or gCTF's local mode (with evenly spaced coordinates instead of real particle locations) would work well. With cryoSPARC there's an easy method to plot the tilt axis; you can also choose a specific number X and Y divisions for the patches.

Best,
-da

On Mon, Feb 28, 2022 at 2:41 PM Morgan, David Gene <dagmorga at indiana.edu<mailto:dagmorga at indiana.edu>> wrote:
Hi,

What's the best way to evaluate the CTF of an image that has a significant amount of tilt?

--
    politics is more difficult than physics.
                                             A. Einstein

            David Gene Morgan
        Electron Microscopy Center
             047E Simon Hall
             IU Bloomington
          812 856 1457 (office)
          812 856 3221 (3200)
      https://urldefense.proofpoint.com/v2/url?u=http-3A__iubemcenter.indiana.edu&d=DwIFAg&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=XfGX1GAOnfj3mLhTnyN8uolpwG3BMtMXZYm0H1sLXZy6LYulpNRf8T8jKuxWTOju&s=qBVmiqzIFHPhsmMv2GVKS3tE0Y3k35YCkiZM5CmZ2cg&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__iubemcenter.indiana.edu&d=DwQFAw&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=o5hWD3mEM1CnBQcA1CoCbNa2ZHuekOPBxJopTggUSnV5bTDS_itRzHiJgm-MqSkZ&s=fCFZWGmlhcc6nzBlB6MWW5ml9YVPIK3FHs7zbhuCqIs&e=>
_______________________________________________
3dem mailing list
3dem at ncmir.ucsd.edu<mailto:3dem at ncmir.ucsd.edu>
https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20220228/198c3f0a/attachment.html>