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Daniel,</div>
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The problem is that the tilt causes the higher resolution Thon rings to become out-of-phase, and so the standard programs can't give a decent estimate of the goodness of-fit. I vaguely remember that someone had a program that took the tilt into account a number
of years ago, but a quick hunt for it didn't turn up anything.<br>
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David Gene Morgan<br>
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<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> Daniel Asarnow <asarnow@msg.ucsf.edu><br>
<b>Sent:</b> Monday, February 28, 2022 5:45 PM<br>
<b>To:</b> Morgan, David Gene <dagmorga@indiana.edu><br>
<b>Cc:</b> 3dem@ncmir.ucsd.edu <3dem@ncmir.ucsd.edu><br>
<b>Subject:</b> [External] Re: [3dem] tilted transfer functions?</font>
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<div>I think any patch-based CTF program, like the one in cryosparc or gCTF's local mode (with evenly spaced coordinates instead of real particle locations) would work well. With cryoSPARC there's an easy method to plot the tilt axis; you can also choose a
specific number X and Y divisions for the patches.</div>
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<div>Best,</div>
<div>-da<br>
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<div dir="ltr" class="x_gmail_attr">On Mon, Feb 28, 2022 at 2:41 PM Morgan, David Gene <<a href="mailto:dagmorga@indiana.edu">dagmorga@indiana.edu</a>> wrote:<br>
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Hi,</div>
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What's the best way to evaluate the CTF of an image that has a significant amount of tilt?<br>
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<div name="x_divtagdefaultwrapper"> politics is more difficult than physics.</div>
<div name="x_divtagdefaultwrapper"> A. Einstein</div>
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<div name="x_divtagdefaultwrapper"> David Gene Morgan<br>
Electron Microscopy Center<br>
047E Simon Hall<br>
IU Bloomington<br>
812 856 1457 (office)<br>
812 856 3221 (3200)<br>
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